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Synergetic effects of epidermal growth factor and gonadotropins on the cytoplasmic maturation of pig oocytes in a serum–free medium

Published online by Cambridge University Press:  26 September 2008

Weihua Wanga  and
Koji Niwa
Weihua Wanga*
Affiliation:
Okayam University, Okayama 700, Japan.
Koji Niwa
Affiliation:
Okayam University, Okayama 700, Japan.
*
Weihua Wang, Laboratory of Embryology, Department of Reproduction, National Institute of Animal Industry, Tsukuba Norindanchi PO Box 5, Ibaraki, 305 Japan. Telephone: 0298-38-8636. Fax: 0298-38-8606.
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Summary

Cumulus-enclosed or cumulus-free pig oocytes were cultured for 48 h in a serum-free maturation medium, TCM-199B containing 1 mg/ml polyvinylalcohol, supplemented with gonadotropins (PMSG + hCG) and/or epidermal growth factor (EGF), and inseminated with frozen-thawed ejaculated spermatozoa. Fourteen hours after insemination, the ability of oocytes to form male pronucleus (MPN) was examined. EGF significantly increased the ability of cumulus-enclosed oocytes to form MPN irrespective of the concentrations (1–100 ng/ml) tested. Gonadotropins had a greater effect than EGF on MPN formation in cumulus-enclosed oocytes, and a synergetic effect was observed when EGF and gonadotropins were added together. However, neither EGF nor gonadotropins exhibited a stimulatory effect on MPN formation in cumulus-free oocytes. These results indicate that the role(s) of both EGF and gonadotropins in stimulating cytoplasmic maturation of pig oocytes is mediated by cumulus.

Keywords

EGFGonadotropinOocyte cytoplasmic maturationPigSerum-free medium
Type
Article
Information
Zygote , Volume 3 , Issue 4 , November 1995 , pp. 345 - 350
Copyright
Copyright © Cambridge University Press 1995

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