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Traditional intracytoplasmic sperm injection provides equivalent outcomes compared with human zona pellucida-bound selected sperm injection

Published online by Cambridge University Press:  09 May 2013

Valentina Casciani ,
Maria Giulia Minasi ,
Gemma Fabozzi ,
Filomena Scarselli ,
Alessandro Colasante ,
Anna Maria Lobascio  and
Ermanno Greco
Valentina Casciani
Affiliation:
Center for Reproductive Medicine, European Hospital, Via Portuense 700 – 00149 Rome, Italy.
Maria Giulia Minasi
Affiliation:
Center for Reproductive Medicine, European Hospital, Via Portuense 700 – 00149 Rome, Italy.
Gemma Fabozzi*
Affiliation:
Center for Reproductive Medicine, European Hospital, Via Portuense 700 – 00149 Rome, Italy.
Filomena Scarselli
Affiliation:
Center for Reproductive Medicine, European Hospital, Via Portuense 700 – 00149 Rome, Italy.
Alessandro Colasante
Affiliation:
Center for Reproductive Medicine, European Hospital, Via Portuense 700 – 00149 Rome, Italy.
Anna Maria Lobascio
Affiliation:
Center for Reproductive Medicine, European Hospital, Via Portuense 700 – 00149 Rome, Italy.
Ermanno Greco
Affiliation:
Center for Reproductive Medicine, European Hospital, Via Portuense 700 – 00149 Rome, Italy.
*
All correspondence to: Gemma Fabozzi. Center for Reproductive Medicine, European Hospital, Via Portuense 700 – 00149 Rome, Italy. Tel: +39 06 65975659. fax: +39 06 6534897. e-mail: gemmafabozzi@hotmail.it
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Summary

The capability of human zona pellucida (ZP) to bind selectively to normal functional sperm with normal chromatin has been reported widely in the literature. The aim of this study was to evaluate whether ZP–binding sperm selection may represent a method to retrieve superior spermatozoa for intracytoplasmic sperm injection (ICSI). Patients were divided into two groups: a ZP-ICSI and a conventional ICSI group. In the ZP-ICSI group, spermatozoa for injection were selected after ZP–sperm incubation and spermatozoa that were tightly bound to the ZP were used for ICSI (ZP-ICSI). Clinical outcomes of ZP-ICSI were compared with the outcomes of traditional scientist-selected sperm injection (conventional ICSI). Results did not show any significant difference in fertilization, pregnancy, implantation and take-home-baby rates between conventional ICSI and ZP-ICSI. However, when data relative to patients who received ZP-ICSI were analyzed, an interesting result was observed: higher sperm concentration and morphology correlated with higher ZP–sperm binding. Additionally, patients with higher ZP–sperm binding seem to have improved pregnancy and take-home-baby rates. In conclusion, this study shows that ZP-ICSI is not a superior method compared with conventional ICSI. However, clinical ICSI outcomes were apparently improved in the presence of good ZP–sperm binding. We therefore speculate that sperm competence to ICSI could be reduced when the sperm's ability to bind the ZP is impaired.

Keywords

Sperm selectionZona pellucidaZP-ICSIZP–sperm binding
Type
Research Article
Information
Zygote , Volume 22 , Issue 4 , November 2014 , pp. 565 - 570
Copyright
Copyright © Cambridge University Press 2013 

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