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Absence of beneficial effects on rabbit sperm cell cryopreservation by several antioxidant agents

Published online by Cambridge University Press:  22 August 2013

M.J. Maya-Soriano ,
E. Taberner ,
M. Sabés-Alsina ,
M. Piles  and
M. Lopez-Bejar
M.J. Maya-Soriano
Affiliation:
Department of Animal Health and Anatomy, Veterinary Faculty, Universitat Autònoma de Barcelona, Barcelona, Spain.
E. Taberner
Affiliation:
Department of Animal Health and Anatomy, Veterinary Faculty, Universitat Autònoma de Barcelona, Barcelona, Spain.
M. Sabés-Alsina
Affiliation:
Department of Animal Health and Anatomy, Veterinary Faculty, Universitat Autònoma de Barcelona, Barcelona, Spain.
M. Piles
Affiliation:
Unitat de Cunicultura, Institut de Recerca i Tecnologia Agroalimentàries, Torre Marimón s/n, 08182’ Caldes de Montbui, Barcelona, Spain.
M. Lopez-Bejar*
Affiliation:
Department of Animal Health and Anatomy Faculty of Veterinary, Universitat Autònoma de Barcelona, Edifici V, Campus UAB, 08193 Barcelona, Spain.
*
All correspondence to: M. Lopez-Bejar. Department of Animal Health and Anatomy Faculty of Veterinary, Universitat Autònoma de Barcelona, Edifici V, Campus UAB, 08193 Barcelona, Spain. Tel: +34 93 5814615. Fax: +34 93 5812006. e-mail: manel.lopez.bejar@uab.cat or mj.maya.soriano@gmail.com
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Summary

The generation of reactive oxygen species associated with cryopreservation could be responsible for mammalian sperm damage and the limitable value of stored semen in artificial insemination. The aim of this study was to assess several antioxidant agents supplemented in a commercial freezing extender (Gent B®) in order to improve post-thaw rabbit sperm quality. Ejaculates of 26 New Zealand White rabbit bucks were collected, evaluated and frozen using a conventional protocol. Antioxidant agents were tested at different concentrations: bovine serum albumin (BSA; 5, 30 or 60 mg/ml), retinol (RO; 50, 100 or 200 μM) and retinyl (RI; 0.282 or 2.82 μg/ml). Per cent viability, morphological abnormalities and intact acrosomes were determined using eosin–nigrosin staining. Motility and progressivity were analyzed by computer-assisted sperm analysis (CASA). In general, all sperm quality parameters were negatively affected by the cryopreservation process, the largest effect seen was for total motility. The addition of antioxidant agents did not improve thaw sperm quality. Furthermore, for RI groups a significant decrease in sperm quality parameters was recorded. In conclusion, rabbit sperm quality is negatively affected by the cryopreservation process. To our knowledge this report is the first using these antioxidants to supplement rabbit freezing extender. BSA and RO at concentrations used in the study did not improve sperm quality parameters after thawing, whereas RI supplementation appeared to be toxic. More studies are required to find the appropriate antioxidants necessary and their most effective concentrations to improve rabbit post-thaw sperm quality.

Keywords

Bovine serum albuminRabbitRetinolRetinylSpermatozoa
Type
Research Article
Information
Zygote , Volume 23 , Issue 1 , February 2015 , pp. 1 - 10
Copyright
Copyright © Cambridge University Press 2013 

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