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Activin-A promotes the development of goat isolated secondary follicles in vitro

Published online by Cambridge University Press:  13 August 2013

Cleidson Manoel Gomes da Silva ,
Simone Vieira Castro ,
Luciana Rocha Faustino ,
Giovanna Quintino Rodrigues ,
Ivina Rocha Brito ,
Rafael Rossetto ,
Márcia Viviane Alves Saraiva ,
Claudio Cabral Campello ,
Carlos Henrique Lobo  and
Carlos Eduardo Azevedo Souza
...Show all authors
Cleidson Manoel Gomes da Silva*
Affiliation:
Programa de Pós-Graduação em Ciências Veterinárias (PPGCV), Laboratório de Manipulação de Oócitos e Folículos Pré-Antrais (LAMOFOPA), Universidade Estadual do Ceará (UECE), Av. Paranjana, 1700, Campus do Itaperi, CEP: 60740–000, Fortaleza–CE–Brasil.
Simone Vieira Castro
Affiliation:
Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, CE, Brazil.
Luciana Rocha Faustino
Affiliation:
Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, CE, Brazil.
Giovanna Quintino Rodrigues
Affiliation:
Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, CE, Brazil.
Ivina Rocha Brito
Affiliation:
Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, CE, Brazil.
Rafael Rossetto
Affiliation:
Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, CE, Brazil.
Márcia Viviane Alves Saraiva
Affiliation:
Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, CE, Brazil.
Claudio Cabral Campello
Affiliation:
Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, CE, Brazil.
Carlos Henrique Lobo
Affiliation:
Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, CE, Brazil.
Carlos Eduardo Azevedo Souza
Affiliation:
Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, CE, Brazil.
Arlindo de Alencar Araripe Moura
Affiliation:
Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, CE, Brazil.
Mariana Aragão Matos Donato
Affiliation:
Laboratory of Ultrastructure, CPqAM/Fiocruz, Federal University of Pernambuco, Recife, PE, Brazil.
Christina Alves Peixoto
Affiliation:
Laboratory of Ultrastructure, CPqAM/Fiocruz, Federal University of Pernambuco, Recife, PE, Brazil.
José Ricardo de Figueiredo
Affiliation:
Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, CE, Brazil.
*
All correspondence to: Cleidson Manoel Gomes da Silva. Programa de Pós-Graduação em Ciências Veterinárias (PPGCV), Laboratório de Manipulação de Oócitos e Folículos Pré-Antrais (LAMOFOPA), Universidade Estadual do Ceará (UECE), Av. Paranjana, 1700, Campus do Itaperi, CEP: 60740–000, Fortaleza–CE–Brasil. Tel: +55 85 3101 9852. Fax: +55 85 3101 9840. e-mail: gomesvet@hotmail.com
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Summary

The role of activin-A in follicular development and on the mRNA expression levels of different genes in goat secondary follicles was evaluated. Goat secondary follicles (≥150 μm) were cultured for 18 days under control conditions or with the addition of either 50 or 100 ng/ml activin-A (Experiment 1). The mRNA levels for the genes that code for activin-A, ActR-IA, ActR-IB, ActR-IIA, ActR-IIB, follicle stimulating hormone receptor (FSH-R) and P450 aromatase were measured in each condition (Experiment 2). We observed that after 6 days of culture, the antrum formation rate was higher in cultures with added activin-A than in the cultured control (P < 0.05). The addition of 50 ng/ml activin-A increased the follicular growth rate in the final third of the culture (days 12–18), resulting in a higher percentage of meiosis resumption (P < 0.05). On day 6, the addition of activin-A (50 ng/ml) increased the levels of ActR-IA mRNA compared with the cultured control (P < 0.05). After 18 days, the addition of 50 ng/ml activin-A significantly increased the levels of its own mRNA compared with the non-cultured control. Moreover, this treatment reduced the mRNA levels of P450 aromatase in comparison with the cultured control (P < 0.05). Higher levels of P450 aromatase mRNA were found for both activin-A treatments compared with the non-cultured control (P < 0.05). No difference in estradiol levels was detected among any of the tested treatments. In conclusion, the addition of activin-A to culture medium stimulated early antrum formation as well as an increase in the daily follicular growth rate and the percentage of meiosis resumption.

Keywords

Activin-AGoatIn vitro cultureP450 aromataseSecondary follicle
Type
Research Article
Information
Zygote , Volume 23 , Issue 1 , February 2015 , pp. 41 - 52
Copyright
Copyright © Cambridge University Press 2013 

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