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An additional medium renewal of D4 embryo culture improves the concordance of noninvasive chromosome screening with trophectoderm biopsy

Published online by Cambridge University Press:  22 September 2022

Hong-Xing Li Open the ORCID record for Hong-Xing Li [Opens in a new window] ,
Yan Pang ,
Xue-Hong Zhang ,
Di Cao  and
Xiao-Ling Ma
Hong-Xing Li
Affiliation:
The First School of Clinical Medicine, Lanzhou University, Lanzhou730000, China Reproductive Medical Center, the First Hospital of Lanzhou University, Lanzhou730000, China Key Laboratory for Reproductive Medicine and Embryo of Gansu Province, Lanzhou730000, China
Yan Pang
Affiliation:
PET-CT Center of Gansu Provincial Hospital, Lanzhou730000, China
Xue-Hong Zhang
Affiliation:
The First School of Clinical Medicine, Lanzhou University, Lanzhou730000, China
Di Cao
Affiliation:
The First School of Clinical Medicine, Lanzhou University, Lanzhou730000, China
Xiao-Ling Ma*
Affiliation:
The First School of Clinical Medicine, Lanzhou University, Lanzhou730000, China Reproductive Medical Center, the First Hospital of Lanzhou University, Lanzhou730000, China Key Laboratory for Reproductive Medicine and Embryo of Gansu Province, Lanzhou730000, China
*
Author for correspondence: Xiao-Ling Ma. Reproductive Medicine Center, The First Hospital of Lanzhou University, Lanzhou, 730000, China. E-mail: ldyyszzx@163.com
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Summary

Our research question was to evaluate the chromosome concordance of trophectoderm (TE) biopsy with noninvasive chromosome screening (NICS) using embryo culture medium renewed twice on Day 3 (D3) and Day 4 (D4). In this study, we evaluated 64 cycles with 223 biopsied blastocysts. These were categorized into two groups based on replacing embryo culture medium on D3 (control group) or on D3 and D4 (experimental group). The fundamental characteristics and main outcomes were compared. The concordance rates of NICS results with TE biopsy were determined according to next generation sequencing results. In total, 103 experimental and 120 control embryo cultures were collected, and the euploid status was analyzed using NICS technology. The overall concordance rates with TE biopsy of the experimental and control groups were 0.86 and 0.75, respectively. Statistically significant difference was found between the two groups. An additional medium renewal of the D4 embryo culture can improve the concordance of NICS with TE biopsy.

Keywords

BlastocystCulture mediumEuploidyMorphologyNoninvasive chromosome screening
Type
Research Article
Information
Zygote , Volume 30 , Issue 6 , December 2022 , pp. 849 - 853
Copyright
© The Author(s), 2022. Published by Cambridge University Press

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