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Analysis of Bufo arenarum oviductal secretion during the sexual cycle

Published online by Cambridge University Press:  08 June 2009

Claudia A. Crespo ,
Inés Ramos ,
Marcela F. Medina  and
Silvia N. Fernández
Claudia A. Crespo
Affiliation:
Department of Developmental Biology, National Council for Scientific and Technical Research, National University of Tucumán, Chacabuco 461, Tucumán 4000, Argentina.
Inés Ramos
Affiliation:
Department of Developmental Biology, National Council for Scientific and Technical Research, National University of Tucumán, Chacabuco 461, Tucumán 4000, Argentina.
Marcela F. Medina
Affiliation:
Department of Developmental Biology, National Council for Scientific and Technical Research, National University of Tucumán, Chacabuco 461, Tucumán 4000, Argentina.
Silvia N. Fernández*
Affiliation:
Department of Developmental Biology, National University of Tucumán, Chacabuco 461, Tucumán 4000, Argentina. Department of Developmental Biology, National Council for Scientific and Technical Research, National University of Tucumán, Chacabuco 461, Tucumán 4000, Argentina.
*
All correspondence to: Silvia N. Fernández. Department of Developmental Biology, National University of Tucumán, Chacabuco 461, Tucumán 4000, Argentina. Tel: +54 0381 4247752 (7005). Fax: +54 0381 4107214. e-mail: sfernandez@fbqf.unt.edu.ar
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Summary

Bufo arenarum oocytes are oviposited surrounded by jelly coats, one component of the extracellular matrix required for fertilization. The secretion, released to the oviductal lumen, was analysed by SDS-PAGE. The coomassie blue staining evidenced an electrophoretic pattern with molecules ranging between 300 and 19 kDa that showed variations in their secretion profiles during the sexual cycle. In the preovulatory period the densitometric analysis showed the presence of nine peaks with marked predominance of the 74 kDa molecule. Once ovulation has occurred, the jelly coats become arranged around the oocytes during their transit throughout the oviductal pars convoluta (PC), revealing the addition of three proteins only observed during this period, which suggests a differential secretion. Some of these proteins could not diffuse under any extraction treatment, indicating for them a structural or in situ function. Proteins of low molecular mass diffused totally while others showed a partial diffusing capacity. After ovulation a marked decrease in the relative amount of all the proteins released to the lumen, especially the 74 kDa protein, could be detected. During this period, unlike the other stages of the sexual cycle, a differential secretion pattern was observed along the PC. The histochemical analysis performed during the ovulatory period showed the presence of glycoconjugates including both acidic and neutral groups. The present results are in agreement with previous ultrastructural and histochemical studies that describe the role of Bufo arenarum jelly coats in fertilization.

Keywords

AmphibianExtracellular matrixJelly coatsOviductal secretionSexual cycle
Type
Research Article
Information
Zygote , Volume 17 , Issue 4 , November 2009 , pp. 329 - 340
Copyright
Copyright © Cambridge University Press 2009

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