Hostname: page-component-cd9895bd7-jn8rn Total loading time: 0 Render date: 2024-12-27T09:14:41.089Z Has data issue: false hasContentIssue false
  • English
  • Français

Artificial activation of bovine and equine oocytes with cycloheximide, roscovitine, strontium, or 6-dimethylaminopurine in low or high calcium concentrations

Published online by Cambridge University Press:  23 January 2013

Claudia Barbosa Fernandes ,
Liani Gasparini Devito ,
Lilian Rigatto Martins ,
Ieda Dala Pria Blanco ,
João Ferreria de Lima Neto ,
Patricia Myakawa Tsuribe ,
Camila Gabriela Pereria Gonçalves  and
Fernanda da Cruz Landim-Alvarenga
Claudia Barbosa Fernandes*
Affiliation:
Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo (USP), Prof. Dr Orlando Marques de Paiva, 87 Postal code: 05508 270–São Paulo/SP–Brazil.
Liani Gasparini Devito
Affiliation:
Department of Animal Reproduction and Veterinary Radiology, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Rubião Júnior District s/n.–Postal code: 18618–970–Botucatu–SP, Brazil.
Lilian Rigatto Martins
Affiliation:
Department of Animal Reproduction and Veterinary Radiology, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Rubião Júnior District s/n.–Postal code: 18618–970–Botucatu–SP, Brazil.
Ieda Dala Pria Blanco
Affiliation:
Department of Animal Reproduction and Veterinary Radiology, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Rubião Júnior District s/n.–Postal code: 18618–970–Botucatu–SP, Brazil.
João Ferreria de Lima Neto
Affiliation:
Department of Animal Reproduction and Veterinary Radiology, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Rubião Júnior District s/n.–Postal code: 18618–970–Botucatu–SP, Brazil.
Patricia Myakawa Tsuribe
Affiliation:
Endogin, Comandante João Ribeiro de Barros, Km 226, Bauru–SP, Brazil.
Camila Gabriela Pereria Gonçalves
Affiliation:
Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo (USP), Prof. Dr Orlando Marques de Paiva, 87 Postal code: 05508 270–São Paulo/SP–Brazil.
Fernanda da Cruz Landim-Alvarenga
Affiliation:
Department of Animal Reproduction and Veterinary Radiology, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Rubião Júnior District s/n.–Postal code: 18618–970–Botucatu–SP, Brazil.
*
All correspondence to: Claudia Barbosa Fernandes. Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo (USP), Prof. Dr Orlando Marques de Paiva, 87 Postal code: 05508 270–São Paulo/SP–Brazil. Tel: +55 113091 1296. Fax: +55 113091 7412. e-mail: fernandescb@usp.br
Get access Rights & Permissions [Opens in a new window]

Summary

Knowledge on parthenogenetic activation of oocytes is important to improve the efficiency of nuclear transfer (NT) and intracytoplasmic sperm injection (ICSI) because artificial activation of oocyte (AOA) is an essential step to achieve embryo production. Although different procedures for AOA have been established, the efficiency of in vitro production of embryos remains low, especially in equines and Bos taurus bovines. In an attempt to improve the techniques of NT and ICSI in bovine and equine species, we tested different combinations of drugs that had different mechanisms of action for the parthenogenetic activation of oocytes in these animals. The oocytes were collected, in vitro matured for 24 to 30 h and activated artificially, in the presence of low or high concentrations of calcium, with combinations of calcium ionophore (ionomycin) with cycloheximide, roscovitine, strontium, or 6-dimethylaminopurine (6-DMAP). For assessment of activation rates, oocytes were stained with Hoechst 33342 and observed under an inverted microscope. We showed that all combinations of drugs were equally efficient in activating bovine oocytes, with the best results obtained when high concentrations of calcium were adopted. For equine oocytes, high concentrations of calcium were not beneficial for the parthenogenetic activation and the combination of ionomycin with either 6-DMAP or roscovitine was effective in inducing artificial activation of oocytes. We believe that our preliminary findings provide some clues for the development of a better AOA protocol to be used with these species.

Keywords

CloningICSINuclear transferParthenogenetic activation
Type
Research Article
Information
Zygote , Volume 22 , Issue 3 , August 2014 , pp. 387 - 394
Copyright
Copyright © Cambridge University Press 2013 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Aoki, F., Hara, K.T. & Schultz, R.M. (2003). Acquisition of transcriptional competence in the 1-cell mouse embryo: requirement for recruitment of maternal mRNAs. Mol. Reprod. Dev. 64, 270–4.CrossRefGoogle ScholarPubMed
Bedford, S.J., Kurokawa, M., Hinrichs, K. & Fissore, R.A. (2003). Intracellular calcium oscillations and activation in horse oocytes inject with stallion sperm extracts or spermatozoa. Reproduction 126, 489–99.CrossRefGoogle ScholarPubMed
Bos-Mikich, A., Woods, M.J., Candy, C.J. & Whittinghan, D.G. (1995). Cytogenetical analyses and developmental potential of vitrified mouse oocytes. Biol. Reprod. 53, 780–5.CrossRefGoogle Scholar
Bos-Mikich, A., Whittinghan, D.G. & Jones, K.T. (1997). Meiotic and mitotic Ca2+ oscillations affect cell composition in resulting blastocysts. Dev. Biol. 182, 172–9.CrossRefGoogle ScholarPubMed
Carneiro, G.F., Lorenzo, P.L., Pimentel, C., Pegorato, L., Bertolini, M., Ball, B., Anderson, G. & Liu, I. (2001). Influence of insulin-like growth factor-I and its interaction with maturation and parthenogenic development in equine oocytes. Biol. Reprod. 65, 899–905.CrossRefGoogle ScholarPubMed
Choi, Y.H., Love, L.B., Varner, D.D. & Hinrichs, K. (2006). Blastocysts development in equine oocytes with low meiotic competence after suppression of meiosis with roscovitine prior in vitro maturation. Zygote 14, 1–8.CrossRefGoogle ScholarPubMed
Cuthbertson, K.S.R., Whittingham, D.G. & Cobbold, P.H. (1981). Free Ca2+ increases in exponential phases during mouse oocytes activation. Nature 294, 754–7.CrossRefGoogle Scholar
Dolmetsch, R.E., Lewis, R.S., Goodnow, C.C. & Healy, J.I. (1997). Differential activation of transcription factors induced by Ca response amplitude and duration. Nature 386, 855–8.CrossRefGoogle Scholar
Flament, S., Bodart, J.F., Bertout, M., Browayes, E., Rousseau, A. & Vilain, J.P. (2000). Differential effects of 6DMAP, olomoucine and roscovitine on Xenopus oocytes and eggs. Zygote 8, 3–14.CrossRefGoogle ScholarPubMed
Franz, L., Choi, Y.H., Squires, E.L., Seidel, G.E. Jr & Hinrichs, K. (2003). Effect of roscovitine on maintenance of germinal vesicle in horse oocytes, subsequent nuclear maturation, and cleavage rates after intracytoplasmic sperm injection. Reproduction 125, 693–700.CrossRefGoogle ScholarPubMed
Galli, C., Colleoni, S., Duchi, R., Lagutina, I. & Lazzri, G. (2007). Developmental competence of equine oocytes and embryos obtained by in vitro procedures ranging from in vitro maturation and ICSI to embryo culture cryopreservation and somatic cell nuclear transfer. Anim. Reprod. Sci. 98, 39–55.CrossRefGoogle ScholarPubMed
Hinrichs, K., Schmidt, A.L. & Selgrath, J.P. (1995). Activation of horse oocytes. Biol. Reprod. Mono. 1, 319–24.CrossRefGoogle Scholar
Hinrichs, K., Choi, Y.H., Love, C.C., Chung, Y.G. & Varner, D.D. (2006). Production of horse foals via direct injection or roscovitine-treated donor cells and activation by injection of sperm extract. Reproduction 131, 1063–72.CrossRefGoogle ScholarPubMed
Hinrichs, K., Choi, Y.H., Walckenaer, D.D., Varner, D.D. & Hartman, D.L. (2007). In vitro produced equine embryos: production of foals after transfer, assessment by differential staining and effect of medium calcium concentration during culture. Theriogenology 68, 521–9.CrossRefGoogle ScholarPubMed
Homa, S.T. (1995). Calcium and meiotic maturation of the mammalian oocyte. Mol. Reprod. Dev. 40, 122–34.CrossRefGoogle ScholarPubMed
Jellerette, T., He, C.L., Wu, H., Parys, J.B. & Fissore, R.A. (2000). Downregulation of the inositol 1,4,5-triphosphate receptor in mouse eggs following fertilization or parthenogenetic activation. Dev. Biol. 223, 238–50.CrossRefGoogle ScholarPubMed
Kim, N.H., Simerly, C., Funahashi, H., Schatten, G. & Day, B.N. (1996). Microtubule organization in porcine oocytes during fertilization and parthenogenesis. Biol. Reprod. 54, 1397–404.CrossRefGoogle ScholarPubMed
Kline, D. & Kline, J.T. (1992). Repetitive calcium transients and the role of calcium in exocytosis and cell cycle activation in the mouse egg. Dev Biol. 149, 80–9.CrossRefGoogle ScholarPubMed
Li, W., Lopis, J., Whitney, M., Zlokarnik, G. & Tsien, R.Y. (1998). Cell permanent caged InsP3 ester shows that Ca spike frequency can optimize gene expression. Nature 392, 936–41.CrossRefGoogle Scholar
Li, X., Morris, L.H.A. & Allen, W.R. (2000). Chromatin reprogramming in enucleated horse oocytes injected with cumulus cell nuclei. J. Reprod. Fertil. Abstract Series 25, Abstract 77.Google Scholar
Liu, L. & Yang, X. (1999). Interplay of maturation promoting factor and mitogen activated protein kinase inactivation during metaphase to interphase transition of activated bovine oocytes. Biol. Reprod. 61, 1–7.CrossRefGoogle ScholarPubMed
Loi, P., Ledda, S., Fulka, J., Cappai, P. & Moor, R.M. (1998). Development of parthenogenetic and cloned ovine embryos: effect of activation protocols. Biol. Reprod. 58, 1177–87.CrossRefGoogle ScholarPubMed
Marchal, R., Tomanek, M., Terqui, M,. & Mermillod, P. (2001). Effects of cell cycle dependent kinases inhibitor on nuclear and cytoplasmic maturation of porcine oocytes. Mol. Reprod. Dev. 60, 65–73.CrossRefGoogle ScholarPubMed
Meo, S.C., Leal, C.L. & Garcia, J.M. (2004). Activation and early parthenogenesis of bovine oocytes treated with ethanol and strontium. Anim. Reprod. Sci. 81, 35–46.CrossRefGoogle ScholarPubMed
Meo, S.C., Yamazaki, W., Leal, C.L.V., Oliveira, J.A. & Garcia, J.M. (2005). Use of strontium for bovine oocyte activation. Theriogenology 63, 2089–102.CrossRefGoogle ScholarPubMed
Mermillod, P., Tomanek, M., Marchal, R. & Meijer, L. (2000). High developmental competence of cattle oocytes maintained at the germinal vesicle stage for 24 h in culture by specific inhibition of MPF kinase activity. Mol. Reprod. Dev. 55, 89–95.3.0.CO;2-M>CrossRefGoogle Scholar
Mitalipov, S.M., Nuisser, K.D. & Wolf, D.P. (2001). Cycloheximide induced activation of mouse eggs: effects on cdc2/cyclin B and MAP Kinase activities. J. Cell Sci. 109, 739–48.Google Scholar
Nakada, K. & Mizuno, J. (1998). Intracellular calcium responses in bovine oocytes induced by spermatozoa and by reagents. Theriogenology 50, 269–82.CrossRefGoogle ScholarPubMed
Oikawa, T., Takada, N., Kikuchi, T, Numabe, T., Takenaka, M. & Horiuchi, T. (2005). Evaluation of activation treatments for blastocyst production and birth of viable claves following bovine intracytoplasmic sperm injection. Anim. Reprod. Sci. 86, 187–94.CrossRefGoogle Scholar
Pimentel, AM., Bordignon, V. & Smith, LC. (2002). Effect of meiotic resumption delay on in vitro maturation and parthenogenic development of equine oocytes. Theriogenology 57, 735.Google Scholar
Prather, R.S., Hawley, R.J., Carter, D.B., Lai, L. & Greenstein, J.L. (2003). Transgenic swine for biomedicine and agriculture. Theriogenology 59, 115–23.CrossRefGoogle ScholarPubMed
Presicce, G.A. & Yang, X. (1994). Nuclear dynamics of parthenogenesis of bovine oocytes matured in vitro for 20 and 40 hours and activated with combined ethanol and cycloheximide treatment. Mol. Reprod. Dev. 37, 61–8.CrossRefGoogle ScholarPubMed
Saunders, M., Larman, G., Parrington, J., Cox, J., Royse, J. & Blayney, M. (2002). PLCzeta: a sperm specific trigger of Ca2+ oscillations in egg and embryo development. Development 129, 3533–44.CrossRefGoogle Scholar
Saunders, C.M., Swann, K. & Lai, F.A. (2007). PLCzeta a sperm specific PLC and its potential role in fertilization. Biochem. Soc. Symp. 74, 23–6.Google Scholar
Vajta, G., Lewis, IM., Trounson, A.O., Purup, S., Maddox-Hyttel, P., Schmidt, M., Petersen, H.G., Greve, T. & Callesen, H. (2003). Handmade somatic cell cloning in cattle: analysis of factors contributing to high efficiency in vitro . Biol Reprod. 68, 571–8.CrossRefGoogle ScholarPubMed
White, K.L. & Yue, C. (1996). Intracellular receptors and agents that induce activation in bovine oocytes. Theriogenology 45, 91–100.CrossRefGoogle Scholar
Woods, G.L., White, K.L., Vanderwall, D.K., Aston, K.I., Bunch, T.D. & Campbell, K.D. (2002). Cloned mule pregnancies produced using nuclear transfer. Theriogenology 58, 779–82.Google Scholar
Yanagimachi, R. (1994). Mammalian fertilization. In The Physiology of Reproduction, 2nd edn (ed. Knobil, E. & Neil, J.), pp. 189–317. New York: Raven Press.Google Scholar