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Beneficial effect of medium with high concentration serum for direct sperm injection into mouse oocytes using a conventional pipette

Published online by Cambridge University Press:  26 September 2008

Kazuo Suzuki  and
R. Yanagimachi
Kazuo Suzuki
Affiliation:
Department of Anatomy and Reproductive Biology, University of Hawaii School of Medicine, Honolulu, Hawaii 96822, USA
R. Yanagimachi*
Affiliation:
Department of Anatomy and Reproductive Biology, University of Hawaii School of Medicine, Honolulu, Hawaii 96822, USA
*
R. Yanagimachi, Department of Anatomy and Reproductive Biology, University of Hawaii School of Medicine, 1951 East-West Road, Honolulu, HI 96822USA. Fax: +1(808)956-5474. e-mail: yana@hawaii.edu.
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Summary

Although the piezo-electrically driven pipette is much more effective than a conventional pipette for direct sperm injection into mouse oocytes, the piezo-pipette driving unit is expensive and may not be readily accessible to most basic laboratories. We reprot here that the inefficiency of the conventional pipette can be improved by performing the operation in a medium with high serum concentration.

Keywords

Intracytoplasmic sperm injectionOocyteSerumSperm
Type
Article
Information
Zygote , Volume 5 , Issue 2 , May 1997 , pp. 111 - 116
Copyright
Copyright © Cambridge University Press 1997

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