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Chilling curves for Piaractus mesopotamicus (Holmberg, 1887) embryos stored at −8°C

Published online by Cambridge University Press:  08 March 2012

Taís da S. Lopes ,
Danilo Pedro Streit Jr ,
Darci Carlos Fornari ,
Diego de Oliveira ,
Ricardo Pereira Ribeiro  and
Elizabeth Romagosa
Taís da S. Lopes*
Affiliation:
Aquaculture Centre, São Paulo State University (UNESP), Via de Acesso Prof. Paulo Donato Castellane, s/n, CEP 14884-900, Jaboticabal, SP, Brazil.
Danilo Pedro Streit Jr
Affiliation:
Fishery Institute – APTA, SAA, São Paulo, SP, Brazil.
Darci Carlos Fornari
Affiliation:
Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
Diego de Oliveira
Affiliation:
Fishery Institute – APTA, SAA, São Paulo, SP, Brazil.
Ricardo Pereira Ribeiro
Affiliation:
Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
Elizabeth Romagosa
Affiliation:
State University of Maringá, Maringá, PR, Brazil.
*
All correspondence to: Taís da S. Lopes. Aquaculture Centre, São Paulo State University (UNESP), Via de Acesso Prof. Paulo Donato Castellane, s/n, CEP 14884-900, Jaboticabal, SP, Brazil. e-mail: tais.peixegen@gmail.com
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Summary

The present study investigates the effect of different slow chilling curves on the storage of pacu (Piaractus mesopotamicus) embryos submitted to chilling at −8°C. Embryos at the blastopore closure stage were divided into two groups: G1 – embryos exposed to cryoprotectant solution containing methanol (10%) and sucrose (0.5 M), treated as follows: (T1) taken directly from room temperature to the refrigerator without being submitted to the curve; (T2) chilling curve of 0.5°C/min; and (T3) chilling curve of 1°C/min; and G2 – the cryoprotectant solution alone was submitted to these same temperatures, receiving the embryos only after temperature had decreased, corresponding to treatments T4, T5 and T6, respectively. Treatments were kept at −8°C for a period of 6 h. Embryo development was evaluated for each treatment, with six replicates in an entirely randomized design. Survival among embryos not submitted to refrigeration was 94.3 ± 8.05%. Percentage of total larvae (TL) and addled eggs (AE) did not differ statistically between the groups, although percentage of swimming larvae (SL) exhibited higher values in G1 for the 1°C/min curve. Furthermore, when comparing the three chilling curves, a decrease of 1°C/min resulted in the highest TL percentage (90.85%), followed by the 0.5°C/min curve (78.52%). Thus, the use of 1°C/min chilling curves is recommended for P. mesopotamicus embryos stored for 6 h at −8°C.

Keywords

CryopreservationEmbryo chillingLarvae survivalPacu
Type
Research Article
Information
Zygote , Volume 21 , Issue 4 , November 2013 , pp. 345 - 350
Copyright
Copyright © Cambridge University Press 2012 

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