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Chilling curves for Piaractus mesopotamicus (Holmberg, 1887) embryos stored at −8°C

Published online by Cambridge University Press:  08 March 2012

Taís da S. Lopes ,
Danilo Pedro Streit Jr ,
Darci Carlos Fornari ,
Diego de Oliveira ,
Ricardo Pereira Ribeiro  and
Elizabeth Romagosa
Taís da S. Lopes*
Affiliation:
Aquaculture Centre, São Paulo State University (UNESP), Via de Acesso Prof. Paulo Donato Castellane, s/n, CEP 14884-900, Jaboticabal, SP, Brazil.
Danilo Pedro Streit Jr
Affiliation:
Fishery Institute – APTA, SAA, São Paulo, SP, Brazil.
Darci Carlos Fornari
Affiliation:
Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
Diego de Oliveira
Affiliation:
Fishery Institute – APTA, SAA, São Paulo, SP, Brazil.
Ricardo Pereira Ribeiro
Affiliation:
Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
Elizabeth Romagosa
Affiliation:
State University of Maringá, Maringá, PR, Brazil.
*
All correspondence to: Taís da S. Lopes. Aquaculture Centre, São Paulo State University (UNESP), Via de Acesso Prof. Paulo Donato Castellane, s/n, CEP 14884-900, Jaboticabal, SP, Brazil. e-mail: tais.peixegen@gmail.com
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Summary

The present study investigates the effect of different slow chilling curves on the storage of pacu (Piaractus mesopotamicus) embryos submitted to chilling at −8°C. Embryos at the blastopore closure stage were divided into two groups: G1 – embryos exposed to cryoprotectant solution containing methanol (10%) and sucrose (0.5 M), treated as follows: (T1) taken directly from room temperature to the refrigerator without being submitted to the curve; (T2) chilling curve of 0.5°C/min; and (T3) chilling curve of 1°C/min; and G2 – the cryoprotectant solution alone was submitted to these same temperatures, receiving the embryos only after temperature had decreased, corresponding to treatments T4, T5 and T6, respectively. Treatments were kept at −8°C for a period of 6 h. Embryo development was evaluated for each treatment, with six replicates in an entirely randomized design. Survival among embryos not submitted to refrigeration was 94.3 ± 8.05%. Percentage of total larvae (TL) and addled eggs (AE) did not differ statistically between the groups, although percentage of swimming larvae (SL) exhibited higher values in G1 for the 1°C/min curve. Furthermore, when comparing the three chilling curves, a decrease of 1°C/min resulted in the highest TL percentage (90.85%), followed by the 0.5°C/min curve (78.52%). Thus, the use of 1°C/min chilling curves is recommended for P. mesopotamicus embryos stored for 6 h at −8°C.

Keywords

CryopreservationEmbryo chillingLarvae survivalPacu
Type
Research Article
Information
Zygote , Volume 21 , Issue 4 , November 2013 , pp. 345 - 350
Copyright
Copyright © Cambridge University Press 2012 

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References

Ahammad, M.M., Bhattacharyya, D. & Jana, B.B. (2003). Stage-dependent hatching responses of rohu (Labeo rohita) embryos to different concentrations of cryoprotectants and temperatures. Cryobiology 46, 1–16.CrossRefGoogle ScholarPubMed
Ahammad, M.M., Bhattachayya, D. & Jana, B.B. (1998). Effect of different concentrations of cryoprotectant and extender on the hatching of Indian major carp embryos (Labeo rohita, Catla catla, and Cirrhinus mrigala) stored at low temperature. Cryobiology 37, 318–24.CrossRefGoogle ScholarPubMed
Chao, N.H. & Liao, I.C. (2001). Cryopreservation of finfish and shellfish gametes and embryos. Aquaculture 197, 161–89.CrossRefGoogle Scholar
Fahy, G.M., Mac Farlane, D.R., Angell, C.A. & Meryman, H.T. (1984). Vitrification as an approach to cryopreservation. Cryobiology 21, 407–26.CrossRefGoogle ScholarPubMed
Fornari, D.C. (2009). Crioprotetores no resfriamento e congelação de embriões de pacu (Piaractus mesopotamicus) embryos. Master's Dissertation, State University of Maringá, Maringá, 64 pp. [in Portuguese].Google Scholar
Hagedorn, M., Hsu, E., Kleinhans, F.W. & Wildt, D.E. (1997). New approaches for studying the permeability of fish embryos: toward successful cryopreservation. Cryobiology 34, 335–47.CrossRefGoogle ScholarPubMed
Hagedorn, M., Kleinhans, F.W., Artemov, D. & Pilatus, U. (1998). Characterization of a major permeability barrier in the zebrafish embryo. Biol. Reprod. 59, 1240–50.CrossRefGoogle Scholar
Hagedorn, M., Peterson, A., Mazur, P. & Kleinhans, F.W. (2004). High ice nucleation temperature of zebrafish embryos: slow-freezing is not an option. Cryobiology 49, 181–9.CrossRefGoogle Scholar
Lahnsteiner, F. (2009). Factors affecting storage of zebrafish (Danio rerio) embryos; Theriogenology 72, 333–40.CrossRefGoogle ScholarPubMed
Leibo, S.P. (2000). Sources of variation in cryopreservation; In: Cryopreservation in Aquatic Species (eds Tiersch, T.R. & Mazik, P.M.) Baton Rouge World Aquaculture Society, pp. 75–83.Google Scholar
Liu, X.H., Zhang, T. & Rawson, D.M. (2001). Effect of cooling rate and partial removal of yolk on the chilling injury in zebrafish (Danio rerio) embryos. Theriogenology 55, 1719–31.CrossRefGoogle ScholarPubMed
Mazur, P. (1977). The role of intracellular freezing in the death of cells cooled at supraoptimal rates. Cryobiology 14, 251–72.CrossRefGoogle ScholarPubMed
Mazur, P. (1984). Freezing of living cells: mechanisms and implications. Cell. Physiol. 16, C125–42.CrossRefGoogle Scholar
Morris, G.J. & Watson, P.F. (1984). Cold shock injury – a comprehensive bibliography. Cryo-Lett. 5, 352–72.Google Scholar
Neves, P.R. (2008). Use of intracellular and extracellular cryoprotectants in pacu (Piaractus mesopotamicus) embryos. Doctoral Thesis. State University of Maringá, Maringá, 71 pp. [in Portuguese].Google Scholar
Ninhaus-Silveira, A., Foresti, F. & Azevedo, A. (2006). Structural and ultrastructural analysis of embryonic development of Prochilodus lineatus (Valenciennes, 1836) (Characiformes, Prochilodontidae). Zygote 14, 217–29.CrossRefGoogle ScholarPubMed
Polge, C., Smith, A.U. & Parkes, A.S. (1949). Revival of spermatozoa after vitrification and dehydration at low temperature. Nature 164, 666.CrossRefGoogle Scholar
Streit, D.P. Jr (2005). Cryoprotectants and chilling of pacu (P. mesopotamicus) embryos. Doctoral Thesis. State University of Maringá, Maringá, 86 pp. [in Portuguese].Google Scholar
Streit, D.P., Digmayer, M., Ribeiro, R.P., Sirol, R.N., Moraes, G.V.E. & Galo, J.M. (2007). Pacu embryos submitted to different chilling protocols. Pesquisa Agropecuária Brasileira 42, 1119–202 [in Portuguese].Google Scholar
Teodoro, A.J. & Ferraz De Lima, J.A. (1986). Practical observations on the induced reproduction of pacu, Colossoma mitrei (spontaneous egg release). In: Brazilian Symposium on Aquaculture pp. 99–112 [in Portuguese].Google Scholar
Zhang, T., Liu, X. & Rawson, D.M. (2003). Effects of methanol and developmental arrest on chilling injury in zebrafish (Danio rerio) embryos. Theriogenology 59, 1545–56.CrossRefGoogle ScholarPubMed