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Crucial surviving aspects for vitrified in vitro-produced bovine embryos

Published online by Cambridge University Press:  11 July 2012

Mateus José Sudano ,
Daniela Martins Paschoal ,
Tatiana da Silva Rascado ,
Letícia Ferrari Crocomo ,
Luis Carlos Oña Magalhães ,
Alício Martins Junior ,
Rui Machado  and
Fernanda da Cruz Landim-Alvarenga
Mateus José Sudano*
Affiliation:
São Paulo State University – UNESP, School of Veterinary Medicine and Animal Science–FMVZ, Department of Animal Reproduction and Veterinary Radiology, Rubião Jr. s/n°, Botucatu–SP, Brazil.
Daniela Martins Paschoal
Affiliation:
São Paulo State University - UNESP, School of Veterinary Medicine and Animal Science–FMVZ, Department of Animal Reproduction and Veterinary Radiology Rubião Jr. s/n°, Botucatu–SP, Brazil, 18618-970.
Tatiana da Silva Rascado
Affiliation:
São Paulo State University - UNESP, School of Veterinary Medicine and Animal Science–FMVZ, Department of Animal Reproduction and Veterinary Radiology Rubião Jr. s/n°, Botucatu–SP, Brazil, 18618-970.
Letícia Ferrari Crocomo
Affiliation:
São Paulo State University - UNESP, School of Veterinary Medicine and Animal Science–FMVZ, Department of Animal Reproduction and Veterinary Radiology Rubião Jr. s/n°, Botucatu–SP, Brazil, 18618-970.
Luis Carlos Oña Magalhães
Affiliation:
São Paulo State University - UNESP, School of Veterinary Medicine and Animal Science–FMVZ, Department of Animal Reproduction and Veterinary Radiology Rubião Jr. s/n°, Botucatu–SP, Brazil, 18618-970.
Alício Martins Junior
Affiliation:
São Paulo State University - UNESP, School of Veterinary Medicine, Department of Surgical Clinic and Animal Reproduction, Araçatuba-SP, Brazil, 16050-680.
Rui Machado
Affiliation:
Embrapa Southeast Cattle, PO Box 339, 13560-970, São Carlos-SP, Brazil.
Fernanda da Cruz Landim-Alvarenga*
Affiliation:
São Paulo State University – UNESP, School of Veterinary Medicine and Animal Science–FMVZ, Department of Animal Reproduction and Veterinary Radiology, Rubião Jr. s/n°, Botucatu–SP, Brazil.
*
All correspondence to: Fernanda da Cruz Landim-Alvarenga or Mateus José Sudano São Paulo State University – UNESP, School of Veterinary Medicine and Animal Science–FMVZ, Department of Animal Reproduction and Veterinary Radiology, Rubião Jr. s/n°, Botucatu–SP, Brazil, 18618-970. Tel:/Fax: +55 14 38116249. e-mail: mjsudano@gmail.com or fernanda@fmvz.unesp.br
All correspondence to: Fernanda da Cruz Landim-Alvarenga or Mateus José Sudano São Paulo State University – UNESP, School of Veterinary Medicine and Animal Science–FMVZ, Department of Animal Reproduction and Veterinary Radiology, Rubião Jr. s/n°, Botucatu–SP, Brazil, 18618-970. Tel:/Fax: +55 14 38116249. e-mail: mjsudano@gmail.com or fernanda@fmvz.unesp.br
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Summary

The objective of the present study was to correlate some parameters (cleavage, blastocyst production, quality degree score, total cell number, fresh apoptosis and lipid content) with embryo survival after cryopreservation. A total of 1727 in vitro-produced bovine blastocysts were used to establish the parameters (mean ± standard error of the mean (SEM)) for cleavage (85.6 ± 0.8), blastocyst production (39.9 ± 1.4), quality degree score (1.6 ± 0.1), total cell number (140.1 ± 2.9), fresh apoptosis (20.8 ± 1.1) and lipid content (21.3 ± 0.8 droplets). On the same way 1316 blastocysts were vitrified for the determination of post-cryopreservation embryo survival (49.4 ± 1.9). Fresh apoptosis rate and total lipid droplets value were correlated (P < 0.05) with embryo survival after cryopreservation (r = 0.91 and r = 0.59; respectively). However, cleavage, blastocyst production, quality degree score and total cell number were not correlated (P > 0.05) with embryo cryotolerance (r = 0.23, r = 0.38, r = 0.22 and r = 0.28; respectively). Therefore, the increased lipid content was moderately correlated with apoptosis in vitrified blastocysts. On the other hand, increased apoptosis in fresh blastocysts was strongly correlated with apoptosis in vitrified blastocysts, which indicated that the apoptosis rate in fresh embryos was a better parameter than the lipid content to predict post-vitrification embryo survival.

Keywords

ApoptosisCryotoleranceEmbryo qualityLipid contentVitrification
Type
Research Article
Information
Zygote , Volume 22 , Issue 2 , May 2014 , pp. 124 - 131
Copyright
Copyright © Cambridge University Press 2012 

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