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Decondensation of the mouse sperm nucleus within the interphase nucleus

Published online by Cambridge University Press:  15 January 2010

Yasutaka Maeda ,
Hiroko Yanagimachi ,
Hiroyuki Tateno ,
Noriko Usui  and
R. Yanagimachi
Yasutaka Maeda
Affiliation:
University of Hawaii Medical School, USA; Kagoshima City Hosptial, Asahikawe Medical College and Teikyo University School of Medicine, Japan
Hiroko Yanagimachi
Affiliation:
University of Hawaii Medical School, USA; Kagoshima City Hosptial, Asahikawe Medical College and Teikyo University School of Medicine, Japan
Hiroyuki Tateno
Affiliation:
University of Hawaii Medical School, USA; Kagoshima City Hosptial, Asahikawe Medical College and Teikyo University School of Medicine, Japan
Noriko Usui
Affiliation:
University of Hawaii Medical School, USA; Kagoshima City Hosptial, Asahikawe Medical College and Teikyo University School of Medicine, Japan
R. Yanagimachi*
Affiliation:
University of Hawaii Medical School, USA; Kagoshima City Hosptial, Asahikawe Medical College and Teikyo University School of Medicine, Japan
*
R. Yanagimachi, Department of Anatomy and Reproductive Biology, University of Hawaii Medical School, 1951 East-West Road, Honolulu, HI 96822, USA.
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Summary

Sperm nuclei incorporated into the cytoplasm (ooplasm) of fertilised mouse eggs at the pronuclear stage remain condensed, whereas those injected into male or female pronuclei decondense. Similarly, sperm nuclei injected into germinal vesicles of immature oocytes or the nuclei of 2-cell embryos decondense, while those entering the cytoplasm of these oocytes / embryos do not. These facts seem to suggest that factors necessary for the decondensation of sperm nucleus are present in interphase nuclei and are released into the ooplasm during nuclear envelope breakdown. Nucleoplasmin, which is synthesised in the cytoplasm and accumulated within the nucleus, is likely a major candidate for these factors.

Keywords

EggGlutathioneNuclear decondensationNucleoplasminOocyteSperm
Type
Research Article
Information
Zygote , Volume 6 , Issue 1 , February 1998 , pp. 39 - 45
Copyright
Copyright © Cambridge University Press 1998

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