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Differential expression of FSHR and LHR genes and proteins during development of rabbit ovarian follicles

Published online by Cambridge University Press:  02 May 2022

Eman Khalil ,
Mohamed Metwally ,
Hatem Bahgaat ,
Ahmed Kassab Open the ORCID record for Ahmed Kassab [Opens in a new window]  and
Anwar El-Shafey
Eman Khalil
Affiliation:
Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Benha University, Egypt
Mohamed Metwally
Affiliation:
Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Benha University, Egypt
Hatem Bahgaat
Affiliation:
Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Benha University, Egypt
Ahmed Kassab*
Affiliation:
Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Benha University, Egypt
Anwar El-Shafey
Affiliation:
Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Benha University, Egypt
*
Author for correspondence: Ahmed A. Kassab, Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Benha University, Egypt. Tel: +20 1117313734. E-mail: kassab_aa@yahoo.com
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Summary

The development of an ovarian follicle is a complex process at the cellular and molecular level that is mainly regulated by follicle-stimulating hormone receptor (FSHR) and luteinizing hormone receptor (LHR). To elucidate the contribution of these receptors to ovarian follicle development, it is necessary to determine their expression profiles during this biological process. Therefore, this study aimed to investigate the relationship between ovarian development pattern and the differential ovarian expression pattern of FSHR and LHR genes as well as proteins at different developmental stages. Ovaries were collected from 30 New Zealand rabbits at day 0 (birth), week 2 (neonate), week 4 (cub), week 16 (maturity), and day 18 pregnancy. Ovarian histology, and gene as well as protein expression were determined using light microscopy, real-time PCR and western blotting, respectively. The results showed that the expression levels of FSHR mRNA and protein increased coincidently with age and the growth of ovarian follicles. The levels of LHR mRNA and protein remained low from the day of birth until week 4 and became significantly higher by week 16 coinciding with appearance of growing and antral follicles, which have a defined thecal layer. FSHR gene and protein expression decreased with pregnancy, whereas LHR increased, reaching a peak level during pregnancy. It can be concluded that changes in FSHR and LHR gene and protein expression could be related to the growth and development of follicles, indicating the regulatory role for these receptors in rabbit folliculogenesis.

Keywords

FolliculogenesisFSHRLHROvaryRabbit
Type
Research Article
Information
Zygote , Volume 30 , Issue 4 , August 2022 , pp. 577 - 583
Copyright
© The Author(s), 2022. Published by Cambridge University Press

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