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Differential expression of microRNAs in 2-cell and 4-cell mouse embryos

Published online by Cambridge University Press:  26 April 2013

Pei Wang ,
Ji Cui ,
Chun Zhao ,
Lin Zhou ,
Xirong Guo ,
Rong Shen ,
Junqiang Zhang  and
Xiufeng Ling
Pei Wang
Affiliation:
State Key Laboratory of Reproductive Medicine, Department of Reproduction, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing 210004, China.
Ji Cui
Affiliation:
State Key Laboratory of Reproductive Medicine, Department of Reproduction, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing 210004, China.
Chun Zhao
Affiliation:
State Key Laboratory of Reproductive Medicine, Department of Reproduction, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing 210004, China.
Lin Zhou
Affiliation:
State Key Laboratory of Reproductive Medicine, Department of Reproduction, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing 210004, China.
Xirong Guo
Affiliation:
State Key Laboratory of Reproductive Medicine, Department of Reproduction, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing 210004, China.
Rong Shen
Affiliation:
State Key Laboratory of Reproductive Medicine, Department of Reproduction, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing 210004, China.
Junqiang Zhang*
Affiliation:
State Key Laboratory of Reproductive Medicine, Department of Reproduction, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing Medical University, Nanjing, Jiangsu Province, China.
Xiufeng Ling*
Affiliation:
State Key Laboratory of Reproductive Medicine, Department of Reproduction, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing Medical University, Nanjing, Jiangsu Province, China.
*
All correspondence to: Xiufeng Ling or to Junqiang Zhang. State Key Laboratory of Reproductive Medicine, Department of Reproduction, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing Medical University, Nanjing, Jiangsu Province, China. Tel:/Fax: +86 25 52226248. e-mail: xiufengling@hotmail.com or junqianz@yahoo.com.cn.
All correspondence to: Xiufeng Ling or to Junqiang Zhang. State Key Laboratory of Reproductive Medicine, Department of Reproduction, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing Medical University, Nanjing, Jiangsu Province, China. Tel:/Fax: +86 25 52226248. e-mail: xiufengling@hotmail.com or junqianz@yahoo.com.cn.
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Summary

In vitro fertilized (IVF) human embryos have a high incidence of developmental arrest before the blastocyst stage, therefore characterization of the molecular mechanisms that regulate embryo development is urgently required. Post-transcriptional control by microRNAs (miRNAs) is one of the most investigated RNA control mechanisms, and is hypothesized to be involved actively in developmental arrest in preimplantation embryos. In this study, we extracted total RNA from mouse 2-cell and 4-cell embryos. Using a miRNA microarray, 192 miRNAs were found to be differentially expressed in 4-cell embryos and 2-cell embryos; 122 miRNAs were upregulated and 70 were downregulated in 4-cell embryos. The microarray results were confirmed by real-time quantitative RT-PCR for six miRNAs (mmu-miR-467h, mmu-miR-466d-3p, mmu-miR-292–5p, mmu-miR-154, mmu-miR-2145, and mmu-miR-706). Cdca4 and Tcf12 were identified as miR-154 target genes by target prediction analysis. This study provides a developmental map for a large number of miRNAs in 2-cell and 4-cell embryos. The function of these miRNAs and the mechanisms by which they modulate embryonic developmental arrest require further study. The results of this study have potential applications in the field of reproductive medicine.

Keywords

2-cell embryo4-cell embryoDevelopmental arrestmiRNA
Type
Research Article
Information
Zygote , Volume 22 , Issue 4 , November 2014 , pp. 455 - 461
Copyright
Copyright © Cambridge University Press 2013 

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