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The dynamic pattern of PLIN3 in pig oocytes and cumulus cells during in vitro maturation

Published online by Cambridge University Press:  13 December 2017

Mingzhu Xu ,
Yaqiong Zeng ,
Daming Chi ,
Linan Si ,
Xiao Qu  and
Juan Li
Mingzhu Xu
Affiliation:
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing Weigang No. 1, Jiangsu Province 210095, People's Republic of China
Yaqiong Zeng
Affiliation:
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing Weigang No. 1, Jiangsu Province 210095, People's Republic of China
Daming Chi
Affiliation:
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing Weigang No. 1, Jiangsu Province 210095, People's Republic of China
Linan Si
Affiliation:
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing Weigang No. 1, Jiangsu Province 210095, People's Republic of China
Xiao Qu
Affiliation:
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing Weigang No. 1, Jiangsu Province 210095, People's Republic of China
Juan Li*
Affiliation:
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing Weigang No. 1, Jiangsu Province 210095, People's Republic of China
*
All correspondence to: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing Weigang No. 1, Jiangsu Province 210095, People's Republic of China. Tel: +86 18994049308. Fax: +86 025 84395314. E-mail: juanli@njau.edu.cn
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Summary

Lipid droplets (LDs) are the main energy resource for porcine preimplantation embryonic development. PLIN3 has been implicated in LD formation and regulation. Therefore, this study aimed to detect the dynamic pattern of PLIN3 in pig oocytes and cumulus cells (CC) during in vitro maturation (IVM), and to determine the relationship between PLIN3 and LD content. IVM with cumulus-enclosed oocytes (CEO), cumulus-denuded oocytes (DO) and the CCs denuded from the corresponding oocytes (DCC) was performed in porcine follicular fluid (PFF) or PFF-free optimized medium. DO and the DCC were cultured together under the same conditions as described above, while the DO was named DTO and the DCC was named DTCC in this group. Firstly, our results revealed LDs distributed widely in oocytes and CC, while the PLIN3 protein coated these LDs and spread out ubiquitously in the cytoplasm. Secondly, not only the mRNA level but also at protein level of PLIN3 in immature naked oocytes (IO) was higher than that in matured CEO, DO and DTO. Although PLIN3 was expressed at lower levels in CC from immature oocytes (ICC), the protein level of PLIN3 was comparably higher in the ECC and DCC groups. The triglyceride (TG) content in CEO and DO was significantly less abundant compared with that in IO. Therefore, our results indicated that co-culturing of oocytes and CC might affect PLIN3 expression levels in CC but not in oocytes. Lipid accumulation in pig oocytes during maturation might be affected by PLIN3 cross-talk between oocytes and CC.

Keywords

Cumulus cellsLipid dropletOocytesPigPLIN3
Type
Research Article
Information
Zygote , Volume 26 , Issue 1 , February 2018 , pp. 40 - 49
Copyright
Copyright © Cambridge University Press 2017 

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