Hostname: page-component-cd9895bd7-hc48f Total loading time: 0 Render date: 2024-12-27T06:45:21.430Z Has data issue: false hasContentIssue false
  • English
  • Français

Effect of adding heterologous versus homologous bovine seminal plasma prior to cryopreservation on bull sperm quality after thawing

Published online by Cambridge University Press:  05 October 2018

Thanapol Nongbua ,
Essraa M Al-Essawe ,
Anders Edman ,
Anders Johannisson  and
Jane M Morrell
Thanapol Nongbua*
Affiliation:
Division of Reproduction, Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden Faculty of Veterinary Sciences, Mahasarakham University, Maha Sarakham, Thailand
Essraa M Al-Essawe
Affiliation:
Division of Reproduction, Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden Department of Clinical Reproductive Physiology, High Institute of Infertility Diagnosis and Assisted Reproductive Technologies, Al-Nahrain University, Baghdad, Iraq
Anders Edman
Affiliation:
Viking Genetics, Skara, Sweden
Anders Johannisson
Affiliation:
Division of Reproduction, Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden
Jane M Morrell
Affiliation:
Division of Reproduction, Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden
*
Author for correspondence: Jane Morrell. Division of Reproduction, Department of Clinical Sciences, Swedish University of Agricultural Sciences (SLU), Box 7054, Uppsala 75007, Sweden. E-mail: jane.morrell@slu.se
Get access Rights & Permissions [Opens in a new window]

Summary

The aim of this study was to investigate the effect of adding homologous or heterologous bovine seminal plasma (SP) to SP-free sperm samples before freezing on sperm quality after thawing. Ejaculates from bulls of known fertility were used as a source of SP. The SP was removed from further aliquots of the same ejaculates by colloid centrifugation to create SP-free sperm samples; the resuspended sperm pellets were treated with homologous or heterologous SP from high or low fertility bulls at 0%, 1% or 5% before freezing. After thawing, sperm quality was evaluated by computer-assisted sperm analysis and flow cytometry for membrane integrity, reactive oxygen species, chromatin structure, mitochondrial membrane potential and protein tyrosine phosphorylation. Data were analysed using Proc MIXED, SAS®. Post-hoc comparisons were adjusted for multiplicity using Tukey’s method. The addition of SP resulted in significant differences in sperm quality, namely velocity class A, Velocity Straight Line (VSL), Velocity Average Path (VAP), Velocity Curved Line (VCL), Amplitude of Lateral Head Displacement (ALH), Hyperactive (HYP), reactive oxygen species (ROS) production and % DNA fragmentation index (DFI) (P<0.05 for each). Although adding 5% homologous SP from high fertility bulls was beneficial to sperm kinematics, 5% heterologous SP from high fertility bulls had a deleterious effect on chromatin integrity and on sperm velocity. In conclusion, adding SP may have either a beneficial effect or a deleterious effect depending on the individuals involved. It might be feasible to use this method to improve sperm quality in some circumstances.

Keywords

Bovine SPFrozen semenHeterologous seminal plasmaHomologous seminal plasmaSperm quality
Type
Research Article
Information
Zygote , Volume 26 , Issue 5 , October 2018 , pp. 388 - 394
Copyright
© Cambridge University Press 2018 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bromfield, JJ (2016) A role for seminal plasma in modulating pregnancy outcomes in domestic species. Reproduction 152, R223–R232.Google Scholar
Campanholi, SP, Monteiro, FM, Ribeiro Dias, EA, Mercadante, ME, de Paz, CC, Dell’Aqua Junior, JA, Papa, FO, Dell’Aqua, CP, Vantini, R Garcia, JM (2017) Effect of seminal plasma removal before cryopreservation of bovine semen obtained by electroejaculation on semen quality and in vitro fertility. Theriogenology 89, 114–121.Google Scholar
De Graaf, SP, Leahy, T, Marti, J, Evans, G Maxwell, WM (2008) Application of seminal plasma in sex-sorting and sperm cryopreservation Theriogenology 70, 1360–1363.Google Scholar
Evenson, DP, Larson, KL Jost, LK (2002) Sperm chromatin structure assay: its clinical use for detecting sperm DNA fragmentation in male infertility and comparisons with other techniques. J Androl 23, 25–43.Google Scholar
Goodla, L, Morrell, JM, Yusnizar, Y, Stålhammar, H Johannisson, A (2014) Quality of bull spermatozoa after preparation by single-layer centrifugation. J Dairy Sci 97, 2204–2212.Google Scholar
Guthrie, HD Welch, GR (2006) Determination of intracellular reactive oxygen species and high mitochondrial membrane potential in Percoll-treated viable boar sperm using fluorescence-activated flow cytometry. J Anim Sci 84, 2089–2100.Google Scholar
Henault, MA Killian, GJ (1996) Effect of homologous and heterologous seminal plasma on the fertilizing ability zona-free bovine oocytes. J Reprod Fertil 108, 199–204.Google Scholar
Hering, DM, Oleński, K, Ruść, A Kaminski, S (2014) Genome-wide association study for semen volume and total number of sperm in Holstein-Friesian bulls. Anim Reprod Sci 151(3–4), 126–130.Google Scholar
Holden, SA, Fernandez-Fuertes, B, Murphy, EM, Lonergan, P Fair, S (2017) Effect of seminal plasma from high- and low-fertility bulls on cauda epididymal sperm function. Reprod Fertil Dev 29, 2457–2465.Google Scholar
Humblot, P, Decoux, G Dhorne, T (1991) Effects of the sire and district of AI on cow fertility. Reprod Domest Anim 26, 225–234.Google Scholar
Johannisson, A, Morrell, JM, Thorén, J, Jönsson, M, Dalin, AM Rodriguez-Martinez, H. (2009) Colloidal centrifugation with Androcoll-E™ prolongs stallion sperm motility, viability and chromatin integrity. Anim Reprod Sci 116, 119–128.Google Scholar
Kruse, R, Dutta, PC Morrell, JM (2011) Colloid centrifugation removes seminal plasma and cholesterol from boar spermatozoa. Reprod Fertil Dev 23, 858–865.Google Scholar
Kumar, P, Kumar, D, Singh, I Yadev, PS (2012) Seminal plasma proteome: promising biomarkers for bull fertility. Agric Res 1, 78–86.Google Scholar
Littell, CR, Stroup, WW, Milliken, AG, Wolfinger, DR Schabenberger, O (2006) SAS® for Mixed Models 2nd edition.Google Scholar
Marco-Jiménez, F, Vicente, JS Viudes-de-Castro, MP (2008) Seminal plasma composition from ejaculates collected by artificial vagina and electroejaculation in Guirra ram. Reprod Domest Anim 43, 403–408.Google Scholar
Morrell, JM Johannisson, A (2014) Comparison of the effect of heterologous and homologous seminal plasma on motility and chromatin integrity of stallion spermatozoa selected by single layer centrifugation. J Vet Med 2014, 325451.Google Scholar
Morrell, JM, Johannisson, A, Dalin, A-M Rodriguez-Martinez, H (2009) Single layer centrifugation with Androcoll™-E can be scaled-up to allow large volumes of stallion ejaculate to be processed easily. Theriogenology 72, 879–884.Google Scholar
Morrell, JM, Lagerquist, A, Humblot, P Johannisson, A (2017) Effect of single layer centrifugation on reactive oxygen species and sperm mitochondrial membrane potential in cooled stallion semen. Reprod Fertil Dev 29, 1039–1045.Google Scholar
Moura, AA, Koc, H, Chapman, DA Killian, GJ (2006) Identification of proteins in the accessory sex gland fluid associated with fertility indexes of dairy bulls: a proteomic approach. J Androl 27, 201–211.Google Scholar
Nongbua, T, Johannisson, A, Edman, A Morrell, JM (2016) Effect of bovine seminal plasma on sperm motility and chromatin integrity. Anim Reprod Sci 169, 113–114.Google Scholar
O’Flaherty, C, Beconi, M Beorlegui, N (1997) Effect of natural antioxidants, superoxide dismutase and hydrogen peroxide on capacitation of frozen–thawed bull spermatozoa. Andrologia 29, 269–275.Google Scholar
Olsson, U (2011) Statistics for Life Science 2. Lund. Studentlitteratur.Google Scholar
Piehler, E, Petrunkina, AM, Ekhlasi-Hundrieser, M Töpfer-Petersen, E (2006) Dynamic quantification of the tyrosine phosphorylation of the sperm surface proteins during capacitation. Cytometry Part A 69A, 1062–1070.Google Scholar
Rodriguez-Martinez, H (2003) Laboratory semen assessment and prediction of fertility still Utopia? Reprod Domest Anim 38, 312–318.Google Scholar
Rodriguez-Martinez, H, Kvist, U, Ernerudh, J, Sanz, L Calvete, JJ (2011) Seminal plasma proteins: what role do they play? Am J Reprod Immunol 66(Suppl 1), 11–22.Google Scholar
Sidhu, KS, Mate, KE, Gunasekera, T, Veal, D, Hetherington, L Baker, MA et al. (2004) A flow cytometric assay for global estimation of tyrosine phosphorylation associated with capacitation of spermatozoa from two marsupial species, the tammar wallaby (Macropus eugenii) and the brushtail possum (Trichosurus vulpecula). Reproduction 127, 95–103.Google Scholar