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Effect of androstenedione on the growth and meiotic competence of bovine oocytes from early antral follicles

Published online by Cambridge University Press:  09 November 2011

Hiroaki Taketsuru ,
Yuji Hirao ,
Naoki Takenouchi ,
Kosuke Iga  and
Takashi Miyano
Hiroaki Taketsuru*
Affiliation:
Laboratory of Reproductive Biology, Graduate School of Agricultural Science, Kobe University, Nada-ku, Kobe 657–8501, Japan.
Yuji Hirao
Affiliation:
National Agricultural Research Center for Tohoku Region, Morioka, Iwate 020–0198, Japan.
Naoki Takenouchi
Affiliation:
National Agricultural Research Center for Tohoku Region, Morioka, Iwate 020–0198, Japan.
Kosuke Iga
Affiliation:
National Agricultural Research Center for Tohoku Region, Morioka, Iwate 020–0198, Japan.
Takashi Miyano
Affiliation:
Graduate School of Agricultural Science, Kobe University, Kobe 657–8501, Japan.
*
All correspondence to: Hiroaki Taketsuru. Laboratory of Reproductive Biology, Graduate School of Agricultural Science, Kobe University, Nada-ku, Kobe 657–8501, Japan. Tel: +81 78 803 5806. Fax: +81 78 803 5807. e-mail: htaketsuru@anim.med.kyoto-u.ac.jp
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Summary

Medium that contains 17β-estradiol has been reported to support in vitro growth of bovine oocytes, isolated from early antral follicles, until the final stage. The aim of this study was to determine the effects of androstenedione in medium on such growing bovine oocytes. Oocyte–granulosa cell complexes were collected from early antral follicles and cultured for 14 days in medium supplemented with 17β-estradiol (0, 10 and 100 ng/ml) or androstenedione (0, 10 and 100 ng/ml). The mean diameter of oocytes measured after seeding on the culture substrate was 96.9 μm (n = 191). Either steroid was necessary for maintainance of the organization of oocyte–granulosa cell complexes over the 14-day culture period. In the 17β-estradiol- or the androstenedione-supplemented medium about 80% or 65%, respectively, of viable oocytes were recovered. In both groups the increase in oocyte size was significant after 14 days. The in vitro grown oocytes were cultured for a further 22–24 h for oocyte maturation; 13% and 30% of oocytes grown in the 10 and 100 ng/ml 17β-estradiol-supplemented medium reached metaphase II, respectively; more than 64% of oocytes grown in the androstenedione-supplemented medium matured to metaphase II. These results show that androstenedione, as 17β-estradiol, can maintain the viability of bovine oocyte–granulosa cell complexes and support the growth of oocytes, and that androstenedione promotes the acquisition of oocyte meiotic competence efficiently at a low dose.

Keywords

AndrostenedioneBovineEarly antral follicle17β-EstradiolOocyte growth
Type
Research Article
Information
Zygote , Volume 20 , Issue 4 , November 2012 , pp. 407 - 415
Copyright
Copyright © Cambridge University Press 2011

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