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The effects of brain-derived neurotrophic factor and metformin on in vitro developmental competence of bovine oocytes

Published online by Cambridge University Press:  01 August 2009

So Gun Hong ,
Goo Jang ,
Hyun Ju Oh ,
Ok Jae Koo ,
Jung Eun Park ,
Hee Jung Park ,
Sung Keun Kang  and
Byeong Chun Lee
So Gun Hong
Affiliation:
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul 151–742, Korea.
Goo Jang
Affiliation:
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul 151–742, Korea.
Hyun Ju Oh
Affiliation:
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul 151–742, Korea.
Ok Jae Koo
Affiliation:
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul 151–742, Korea.
Jung Eun Park
Affiliation:
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul 151–742, Korea.
Hee Jung Park
Affiliation:
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul 151–742, Korea.
Sung Keun Kang
Affiliation:
RNL Bio Co., Ltd, Seoul 151–835, Korea.
Byeong Chun Lee*
Affiliation:
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul 151–742, Korea.
*
All correspondence to: Byeong Chun Lee. Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul 151–742, Korea. Tel: +822 880 1269. Fax: +822 873 1269. e-mail: bclee@snu.ac.kr
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Summary

Brain-derived neurotrophic factor (BDNF) signalling via tyrosine kinase B receptors may play an important role in ovarian development and function. It has been reported that metformin elevates the activity of Tyrosine kinase receptors and may amplify BDNF signalling. The objective of this study was to investigate the effect of BDNF during in vitro maturation (IVM) and/or in vitro culture (IVC) (Experiment 1), and to evaluate the collaborative effect of BDNF and metformin treatment on the developmental competence of bovine in vitro fertilized (IVF) embryos (Experiment 2). In Experiment 1, BDNF, which was added to our previously established IVM systems, significantly increased the proportions of MII oocytes at both 10 ng/ml (86.7%) and 100 ng/ml (85.4%) compared with the control (64.0%). However, there was no statistically significant difference in blastocyst development between the control or BDNF-supplemented groups. In Experiment 2, in order to investigate the effect of BDNF (10 ng/ml) and/or metformin (10−5 M) per se, TCM-199 without serum and hormones was used as the control IVM medium. The BDNF (48.3%) and BDNF plus metformin (56.5%) significantly enhanced the proportions of MII oocytes compared with the control (34.4%). Although, BDNF or metformin alone had no effect in embryo development, BDNF plus metformin significantly improved early embryo development to the 8–16-cell stage compared with the control (16.5 vs. 5.5%). In conclusion, the combination of BDNF and metformin may have a collaborative effect during the IVM period. These results could further contribute to the establishment of a more efficient bovine in vitro embryo production system.

Keywords

Bovine oocyteBrain-derived neurotrophic factorIn vitro fertilizationIn vitro maturationMetformin
Type
Research Article
Information
Zygote , Volume 17 , Issue 3 , August 2009 , pp. 187 - 193
Copyright
Copyright © Cambridge University Press 2009

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