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Effects of different cryopreservation methods on post-thaw culture conditions of in vitro produced bovine embryos

Published online by Cambridge University Press:  16 March 2011

Alessandra Corallo Nicacio ,
Renata Simões ,
Fabiola Freitas de Paula-Lopes ,
Flavia Regina Oliveira de Barros ,
Maria Angelica Peres ,
Mayra Elena Ortiz D'Avila Assumpção  and
Jose Antonio Visintin
Alessandra Corallo Nicacio
Affiliation:
Department of Animal Reproduction, College of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil. College of Veterinary Medicine, State University of Maranhão, São Luis, Brazil.
Renata Simões
Affiliation:
Department of Animal Reproduction, College of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil.
Fabiola Freitas de Paula-Lopes
Affiliation:
Department of Animal Reproduction, College of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil.
Flavia Regina Oliveira de Barros
Affiliation:
Department of Animal Reproduction, College of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil.
Maria Angelica Peres
Affiliation:
Department of Animal Reproduction, College of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil.
Mayra Elena Ortiz D'Avila Assumpção
Affiliation:
Department of Animal Reproduction, College of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil.
Jose Antonio Visintin*
Affiliation:
Department of Animal Reproduction, College of Veterinary Medicine and Animal Science, University of São Paulo, Avenida Prof. Dr. Orlando Marques de Paiva, 87–Cidade Universitária–05508–270–São Paulo, SP, Brazil.
*
All correspondence to: José Antonio Visintin. Department of Animal Reproduction, College of Veterinary Medicine and Animal Science, University of São Paulo, Avenida Prof. Dr. Orlando Marques de Paiva, 87–Cidade Universitária–05508–270–São Paulo, SP, Brazil. Tel: +55 11 3091 7915. Fax: +55 11 3091 7412. e-mail: visintin@usp.br
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Summary

The aim of this work was to evaluate the effect of cryopreservation protocols on subsequent development of in vitro produced bovine embryos under different culture conditions. Expanded in vitro produced blastocysts (n = 600) harvested on days 7–9 were submitted to controlled freezing [slow freezing group: 10% ethylene glycol (EG) for 10 min and 1.2°C/min cryopreservation]; quick-freezing [rapid freezing group: 10% EG for 10 min, 20% EG + 20% glycerol (Gly) for 30 s]; or vitrification [vitrification group: 10% EG for 10 min, 25% EG + 25% Gly for 30 s] protocols. Control group embryos were not exposed to cryoprotectant or cryopreservation protocols and the hatching rate was evaluated on day 12 post-insemination. In order to evaluate development, frozen–thawed embryos were subjected to granulosa cell co-culture in TCM199 or SOFaa for 4 days. Data were analyzed by PROC MIXED model using SAS Systems for Windows®. Values were significant at p < 0.05. The hatching rate of the control group was 46.09%. In embryos cultured in TCM199, slow freezing and vitrification group hatching rates were 44.65 ± 5.94% and 9.43 ± 6.77%, respectively. In embryos cultured in SOFaa, slow freezing and vitrification groups showed hatching rates of 11.65 ± 3.37 and 8.67 ± 4.47%, respectively. In contrast, the rapid freezing group embryos did not hatch, regardless of culture medium. The slow freezing group showed higher hatching rates than other cryopreservation groups. Under such conditions, controlled freezing (1.2°C/min) can be an alternative to cryopreservation of in vitro produced bovine embryos.

Keywords

Bovine embryosCryopreservationEmbryo cultureIn vitro productionSlow freezing
Type
Research Article
Information
Zygote , Volume 20 , Issue 2 , May 2012 , pp. 117 - 122
Copyright
Copyright © Cambridge University Press 2011

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References

Assumpção, M.E.A.O., Milazzotto, M.P., Simões, R., Nicacio, A.C., Mello, M.R.B. & Visintin, J.A. (2008). In vitro survival of in vitro-produced bovine embryos cryopreserved by slow freezing, fast freezing and vitrification. Anim. Reprod. 5, 116–20.Google Scholar
Barceló-Fimbres, M. & Seidel, G.E. Jr (2007), Effects of fetal calf serum, phenazine ethosulfate and either glucose or fructose during in vitro culture of bovine embryos on embryonic development after cryopreservation. Mol. Reprod. Dev. 74, 1395–405.CrossRefGoogle ScholarPubMed
Campos-Chillon, L.F., Wlaker, D.J., De La Torre-Sanchez, J. & Seidel, G.E. Jr (2006). In vitro assessment of a direct transfer vitrification procedure for bovine embryos. Theriogenology 65, 1200–14.CrossRefGoogle ScholarPubMed
Corcoran, D., Rizos, D., Fair, T., Evans, A.C.O. & Lonergan, P. (2007). Temporal expression of transcripts related to embryo quality in bovine embryos cultured from the two-cell to blastocysts stage in vitro or in vivo. Mol. Reprod. Dev. 74, 972–7.CrossRefGoogle ScholarPubMed
Fahning, M.L. & Garcia, M.A. (1992). Status of cryopreservation of embryos from domestic animals. Cryobiology 29, 1–18.CrossRefGoogle ScholarPubMed
George, F., Daniaux, C., Genicot, G., Verhaeghe, B., Lambert, P. & Donnay, I. (2008). Set up of a serum-free culture system for bovine embryos: embryo development and quality before and after transient transfer. Theriogenology 69, 612–23.CrossRefGoogle ScholarPubMed
Hochi, S., Seemple, E. & Leibo, S.P. (1996). Effect of cooling and warming rates during cryopreservation on survival of in vitro-produced bovine embryos. Theriogenology 46, 837–47.CrossRefGoogle ScholarPubMed
Jin, B., Kusanagi, K., Ueda, M., Seki, S., Valdez, D.M. Jr, Edashige, K. & Kasai, M. (2008). Formations of extracellular and intracellular ice during warming of vitrified mouse and its effect on embryo survival. Cryobiology 56, 233–40.CrossRefGoogle ScholarPubMed
Kaidi, S., Bernard, S., Lambert, P., Massip, A., Dessy, F. & Donnay, I. (2001). Effect of conventional controlled-rate freezing and vitrification on morphology and metabolism of bovine blastocysts produced in vitro. Biol. Reprod. 65, 1127–34.CrossRefGoogle ScholarPubMed
Kaidi, S., Van Langendockt, A., Massip, A., Dessy, F. & Donnay, I. (1999). Cellular alteration after dilution of cryoprotective solutions used for the vitrification of in vitro-produced bovine embryos. Theriogenology 52, 515–25.CrossRefGoogle ScholarPubMed
Leibo, S.P. (2008). Cryopreservation of oocytes and embryos: optimization by theoretical versus empirical analysis. Theriogenology 69, 37–47.CrossRefGoogle ScholarPubMed
Leoni, G., Bogliolo, L., Berlinguer, F., Rosati, I., Pintus, P.P., Ledda, S. & Naitana, S. (2002). Defined media for vitrification, warming, and rehydration: effects on post-thaw protein synthesis and viability of in vitro derived ovine embryos. Cryobiology 45, 204–12.CrossRefGoogle ScholarPubMed
Lonergan, P., Fair, T., Corcoran, D. & Evans, A.C.O. (2006). Effect of culture environment on gene expression and developmental characteristics in IVF-derived embryos. Theriogenology 26, 137–52.CrossRefGoogle Scholar
Marquez-Alvarado, Y.C., Gallina, C.S., Castilla, B., Leon, H. & Moreno-Mendoza, N. (2004). Evidence of damage in cryopreserved and fresh bovine embryos using the Tunnel technique. Reprod. Dom. Anim. 39, 141–5.CrossRefGoogle Scholar
Martinez, A.G., Valcárcel, A., De Las Heras, M.A., De Matos, D.G., Furnus, C. & Brogliatti, G. (2002). Vitrification of in vitro produced bovine embryos: in vitro and in vivo evaluations. Anim. Reprod. Sci. 73, 11–21.CrossRefGoogle ScholarPubMed
Mello, M.R.B., Queiroz, V.S., Lima, A.S., Tavares, L.M.T., Assumpção, M.E.O.A., Wheeler, M.B. & Visintin, J.A. (2001). Cryopreservation of mouse morulae through different methods: slow-freezing, vitrification and quick-freezing. Braz. J. Vet. Res. Anim. Sci. 38, 160–4.CrossRefGoogle Scholar
Mucci, N., Aller, J., Kaiser, G.G., Hozbor, F., Cabodevilla, J. & Alberio, R.H. (2005). Effect of estrous cow serum during bovine embryo culture on blastocyst development and cryotolerance after slow freezing or vitrification. Theriogenology 65, 1551–62.CrossRefGoogle ScholarPubMed
Nedambale, T.L., Dinnyés, A., Groen, W., Dobrinsky, J.R., Tian, X.C. & Yang, X. (2004). Comparison on in vitro fertilized bovine embryos cultured in KSOM or SOF and cryopreserved by slow freezing or vitrification. Theriogenology 62, 437–49.CrossRefGoogle ScholarPubMed
Pereira, D.C., Dode, M.A.N. & Rumpf, R. (2005). Evaluation of different culture systems on the in vitro production of bovine embryos. Theriogenology 63, 1131–41.CrossRefGoogle ScholarPubMed
Robert, C., McGraw, S., Massicotte, L., Pravetoni, M., Gandolfi, F. & Sirard, M.A. (2002). Quantification of housekeeping transcript levels during the development of bovine preimplantation embryos. Biol. Reprod. 67, 1465–72.CrossRefGoogle ScholarPubMed
Russell, D.F., Baqir, S., Bordignon, J. & Betts, D.H. (2006). The impact of oocyte maturation media on early bovine embryonic development. Mol. Reprod. Dev. 73, 1255–70.CrossRefGoogle ScholarPubMed
Silva, F.M. & Metelo, R. (2005). Relation between physical properties of the zona pellucida and viability of bovine embryos after slow-freezing and vitrification. Reprod. Dom. Anim. 40, 205–9.CrossRefGoogle Scholar
Tavares, L.M.T., Feitosa, W.B., Mello, M.R.B., Nicacio, A.C., Lima, A.S., Assumpção, M.E.A.O. & Visintin, J.A. (2008). Is the early reduction of fetal calf serum concentration in bovine in vitro embryo culture beneficial? Anim. Reprod. 5, 34–8.Google Scholar
Thompson, J.G. (1997). Comparison between in vitro-derived and in vitro-produced pre-elongation embryos from domestic ruminants. Reprod. Fertil. Dev. 9, 341–54.CrossRefGoogle Scholar
Tominaga, K., Iwaki, F. & Hochi, S. (2007). Conventional freezing of in vitro-produced and biopsied bovine blastocysts in the presence of a low concentration of glycerol and sucrose. J. Reprod. Dev. 53, 443–7.CrossRefGoogle ScholarPubMed
Vajta, G., Holm, P., Greve, T. & Callesen, H. (1996). Factors affecting survival rates of in vitro produced bovine embryos after vitrification and direct in-straw rehydration. Anim. Reprod. Sci. 45, 191–200.CrossRefGoogle ScholarPubMed
Van Wagtendonk-De Leeuw, A.M., Den Daas, J.H.G., Kruip, T.H.A.M. & Rall, W.F. (1995). Comparison of the efficacy of conventional slow freezing and rapid cryopreservation methods for bovine embryos. Cryobiology 32, 157–67.CrossRefGoogle ScholarPubMed
Wrenzycki, C., Herrmann, D. & Niemann, H. (2007). Messenger RNA in oocytes and embryos in relation to embryo viability. Theriogenology 68, 77–83.CrossRefGoogle ScholarPubMed