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Effects of EGF and melatonin on gene expression of cumulus cells and further in vitro embryo development in bovines

Published online by Cambridge University Press:  08 June 2022

A. Cordova ,
M. S. Miranda Open the ORCID record for M. S. Miranda [Opens in a new window] ,
W. A. King  and
G. F. Mastromonaco
A. Cordova
Affiliation:
University of Guelph, Department of Biomedical Sciences, 50 Stone Road E, Guelph, ON N1G2W1 Canada Reproductive Physiology, Toronto Zoo Scarborough, ON, Canada
M. S. Miranda*
Affiliation:
Instituto de Medicina Veterinária, Universidade Federal do Pará, BR-316 Km 61, Castanhal, PA 68740-970, Brazil
W. A. King
Affiliation:
University of Guelph, Department of Biomedical Sciences, 50 Stone Road E, Guelph, ON N1G2W1 Canada
G. F. Mastromonaco
Affiliation:
University of Guelph, Department of Biomedical Sciences, 50 Stone Road E, Guelph, ON N1G2W1 Canada Reproductive Physiology, Toronto Zoo Scarborough, ON, Canada
*
Author for correspondence: Moysés dos Santos Miranda. Instituto de Medicina Veterinária, Universidade Federal do Pará, BR-316 Km 61, Castanhal, PA 68740-970, Brazil. E-mail: moyses@ufpa.br
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Summary

Despite previous research demonstrating the benefits of including growth factors and antioxidants to maturation medium to support embryo production, to date the effect of epidermal growth factor (EGF) and melatonin (Mel) on oocyte competency has not been studied. This study supplemented in vitro maturation (IVM) medium with EGF (10 ng/ml) and Mel (50 ng/ml) alone, or in combination, and evaluated cumulus cell (CC) gene expression and the development and quality of parthenogenetic blastocysts. No differences in CC gene expression levels indicative of developmental potential were found among the treatment groups. Antioxidant gene CuZnSOD was significantly (P < 0.05) decreased in CCs from the Mel group. Moreover, blastocyst rates on day 7 were significantly increased in EGF or Mel (P < 0.05), but not EGF+Mel. Significant decrease (P < 0.05) in GPX1, CuZnSOD, SLC2A1 and HSPA1A (P = 0.07) mRNA levels was observed in blastocysts from the Mel group. OCT4 gene expression was significantly increased (P < 0.05) in EGF+Mel and confirmed using immunofluorescence. Our results indicate that, despite the lack of changes of competence-related genes in CCs, IVM medium supplemented with Mel improved the culture environment sufficiently, resulting in improved blastocysts. Moreover, EGF and Mel combined during maturation increased OCT4 gene and protein expression in blastocysts, indicating its potential for stem cells.

Keywords

Cumulus cellsEpidermal growth factorMelatoninOCT4Parthenogenesis
Type
Research Article
Information
Zygote , Volume 30 , Issue 5 , October 2022 , pp. 600 - 610
Copyright
© The Author(s), 2022. Published by Cambridge University Press

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