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Embryo sHLA-G secretion is related to pregnancy rate

Published online by Cambridge University Press:  15 March 2019

R. Rodríguez Díaz ,
R. Blanes Zamora Open the ORCID record for R. Blanes Zamora [Opens in a new window] ,
R. Vaca Sánchez ,
J. González Pérez  and
J.C. Alberto Bethencourt
R. Rodríguez Díaz*
Affiliation:
Department Obstetrics and Gynaecology, Universidad de La Laguna. Reproduction Unit, Hospital Universitario de Canarias, Ctra. Ofra S/N La Cuesta, 38320La Laguna, Santa Cruz de Tenerife
R. Blanes Zamora
Affiliation:
Department Obstetrics and Gynaecology, Universidad de La Laguna. Reproduction Unit, Hospital Universitario de Canarias, Ctra. Ofra S/N La Cuesta, 38320La Laguna, Santa Cruz de Tenerife
R. Vaca Sánchez
Affiliation:
Department Obstetrics and Gynaecology, Universidad de La Laguna. Reproduction Unit, Hospital Universitario de Canarias, Ctra. Ofra S/N La Cuesta, 38320La Laguna, Santa Cruz de Tenerife
J. González Pérez
Affiliation:
Department Obstetrics and Gynaecology, Universidad de La Laguna. Reproduction Unit, Hospital Universitario de Canarias, Ctra. Ofra S/N La Cuesta, 38320La Laguna, Santa Cruz de Tenerife
J.C. Alberto Bethencourt
Affiliation:
Department Obstetrics and Gynaecology, Universidad de La Laguna. Reproduction Unit, Hospital Universitario de Canarias, Ctra. Ofra S/N La Cuesta, 38320La Laguna, Santa Cruz de Tenerife
*
Address for correspondence: Rubí Rodríguez Díaz. Unidad de Reproducción Humana. Hospital Universitario de Canarias (CHUC), Área Obstetricia y Ginecología, Universidad de La Laguna (ULL), Tenerife, Spain. Tel: +34 677 021 762. E-mail: rubrod@ull.edu.es
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Summary

HLA-G expression has been detected in early preimplantation embryos and it has been postulated that a relationship between embryonic expression of this factor and successful pregnancy may exist. Forty-six patients were prospectively selected from our centre ‘Unidad de Reproducción Humana, Hospital Universitario de Canarias’ for conducting this study. In all cases, metaphase II (MII) oocytes were fertilized using intracytoplasmic sperm injection 2–4 h after retrieval. Embryos were cultured individually in 20 µl droplets of G-1 medium (VitroLife) under oil at 37°C and a 6% CO2 environment. Fertilization was assessed at 18 h postinsemination and all oocytes fertilized were passed into a new culture plaque individually in 300 µl culture medium until day 3 of culture. The culture medium was examined for the expression and secretion of sHLA-G with a sandwich enzyme-linked immunosorbent assay kit (BioVendor, Heidelberg, Germany) according to the manufacturer’s instructions. We found statistical significance between higher levels of sHLA-G secretion and pregnancy rate. When both groups were compared there was no difference in embryo quality of transferred embryos, but a significant difference in the number of oocytes and the embryo quality of the cohort existed that was greater in the pregnant group. A standardized sHLA-G assay with a specifically defined range and standard units provides a non-invasive method to identify the most competent embryos for transfer.

Keywords

Embryo cohortEmbryo transferIVF/ICSI outcomePregnancySECRETOMICSSoluble HLA-G
Type
Research Article
Information
Zygote , Volume 27 , Issue 2 , April 2019 , pp. 78 - 81
Copyright
© Cambridge University Press 2019 

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References

Aldrich, CL, Stephenson, MD, Karrison, T, Odem, RR, Branch, DW, Scott, JR, Schrieber, JR and Ober, C (2001) HLA-G genotypes and pregnancy outcome in couples with unexplained recurrent miscarriage. Mol Hum Reprod 7, 1167–1172.Google Scholar
ASEBIR Cuadernos de Embriología Clínica (2015) Criterios ASEBIR de Valoración de Oocitos, Embriones tempranos y Blastocistos Humanos 3rd edition.Google Scholar
Bjorkman, PJ, Saper, MA, Samraoui, B, Bennett, WS, Strominger, JL and Wiley, DC (1987) Structure of the human class I histocompatibility antigen, HLA-A2. Nature 329, 506–512.Google Scholar
Desai, N, Filipovits, J and Goldfarb, J (2006) Secretion of soluble HLA-G by day 3 human embryos associated with higher pregnancy and implantation rates: assay of culture media using a new ELISA kit. Reprod BioMed Online 13, 272–277.Google Scholar
Eskicioğlu, F, Özdemir, AT, Özdemir, RB, Turan, GA, Akan, Z and Hasdemir, SP (2016) The association of HLA-G and immune markers in recurrent miscarriages. J Matern Fetal Neonatal Med 29, 3056–3060.Google Scholar
Fuzzi, B, Rizzo, R, Criscuoli, L, Noci, I, Melchiorri, L, Scarselli, B, Bencini, E, Menicucci, A and Baricordi, OR (2002) HLA-G expression in early embryos is a fundamental prerequisite for the obtainment of pregnancy. Eur J Immunol 32, 311–315.Google Scholar
Giacomini, E, Vago, R, Sánchez, AM, Podini, P, Zarovni, N, Murdica, V, Rizzo, R, Bortolotti, D, Candiani, M and Vigano, P (2017) Secretome of in vitro cultured human embryos contains extracellular vesicles that are uptaken by the maternal side. Sci Rep 7, 5210.Google Scholar
Hackmon, R, Hallak, M, Krup, M, Weitzman, D, Sheiner, E, Kaplan, B and Weinstein, Y (2004) HLA-G antigen and parturition: maternal serum, fetal serum and amniotic fluid levels during pregnancy. Fetal Diagn Ther 19, 404–409.Google Scholar
Jurisicova, A, Casper, RF, MacLusky, NJ, Mills, GB and Librach, CL (1996) HLA-G expression during pre-implantation human embryo development. Proc Natl Acad Sci USA 93, 161–165.Google Scholar
Kotze, D, Kruger, TF, Lombrd, C, Padayachee, T, Keskintepe, L and Sher, G (2013) The effect of the biochemical marker soluble human leukocyte antigen G on pregnancy outcome in assisted reproductive technology – a multicenter study. Fert Steril 100, 1303–1309.Google Scholar
Marchal-Bras-Goncalves, R, Rouas-Freiss, N, Connan, F, Choppin, J, Dausset, J, Carosella, ED, Kirszenbaum, M and Guillet, J (2001) A soluble HLA-G protein that inhibits natural killer cell-mediated cytotoxicity. Transplant Proc 33, 2355–2359.Google Scholar
Martí, S, Ten, J, Marcos, P, Artacho, MJ, Galán, FJ, Bernabeu, R and Rubio, G (2007) Quantifying soluble HLA-G in supernatants of cultured embryos as a marker of implantation potential in an assisted reproduction program. Inmunología 26, 127–134.Google Scholar
Noci, I, Fuzzi, B, Rizzo, R, Melchiorri, L, Criscuoli, L, Dabizzi, S, Biagiotti, R, Pellegrini, S, Menicucci, A and Baricordi, OR (2005) Embryonic soluble HLA-G as a marker of developmental potential in embryos. Hum Reprod 20, 138–146.Google Scholar
Pfeiffer, KA, Fimmers, R, Engels, G, Ven, H and Ven, K (2001) The HLA-G genotype is potentially associated with idiopathic recurrent spontaneous abortion. Mol Hum Reprod 7, 373–378.Google Scholar
Rebmann, V, Switala, M, Eue, I and Grosse-Wilde, H (2010) Soluble HLA-G is an independent factor for the prediction of pregnancy outcome after ART: a German multi-centre study. Hum Reprod 25, 1691–1698.Google Scholar
Rizzo, R, Vercammen, M, van de Velde, H, Horn, PA and Rebmann, V (2011) The importance of HLA-G expression in embryos, trophoblast cells, and embryonic stem cells. Cell Mol Life Sci 68, 341–352.Google Scholar
Roussev, RG and Coulam, CB (2007) HLA-G and its role in implantation (review). J Assist Reprod Genet 24, 288–295.Google Scholar
Sipak-Szmigiel, O, Ronin-Walknowska, E, Cybulski, C, Plonka, T and Lubinski, J (2007) Antigens HLA-G, sHLA-G and sHLA-class I in reproductive failure. Folia Histochem Cytobiol 45, 137–141.Google Scholar
Wiendl, H, Feger, U, Mittelbronn, M, Jack, C, Schreiner, B, Stadelmann, C, Antel, J, Brueck, W, Meyermann, R, Baror, A, Kieseier, BC and Weller, M (2005) Expression of the immune-tolerogenic major histocompatibility molecule HLA-G in multiple sclerosis: implication for CNS immunity. Brain 128(Pt 11), 2689–2701.Google Scholar
Yao, G-D, Shu, Y-M, Shi, S-L, Peng, Z-F, Song, W-Y, Jin, H-X and Sun, Y-P (2014) Expression and potencial roles of HLA-G in human spermatogenesis and early embryonic development. PLoS One 9, e92889.Google Scholar