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Embryo sHLA-G secretion is related to pregnancy rate

Published online by Cambridge University Press:  15 March 2019

R. Rodríguez Díaz ,
R. Blanes Zamora Open the ORCID record for R. Blanes Zamora [Opens in a new window] ,
R. Vaca Sánchez ,
J. González Pérez  and
J.C. Alberto Bethencourt
R. Rodríguez Díaz*
Affiliation:
Department Obstetrics and Gynaecology, Universidad de La Laguna. Reproduction Unit, Hospital Universitario de Canarias, Ctra. Ofra S/N La Cuesta, 38320La Laguna, Santa Cruz de Tenerife
R. Blanes Zamora
Affiliation:
Department Obstetrics and Gynaecology, Universidad de La Laguna. Reproduction Unit, Hospital Universitario de Canarias, Ctra. Ofra S/N La Cuesta, 38320La Laguna, Santa Cruz de Tenerife
R. Vaca Sánchez
Affiliation:
Department Obstetrics and Gynaecology, Universidad de La Laguna. Reproduction Unit, Hospital Universitario de Canarias, Ctra. Ofra S/N La Cuesta, 38320La Laguna, Santa Cruz de Tenerife
J. González Pérez
Affiliation:
Department Obstetrics and Gynaecology, Universidad de La Laguna. Reproduction Unit, Hospital Universitario de Canarias, Ctra. Ofra S/N La Cuesta, 38320La Laguna, Santa Cruz de Tenerife
J.C. Alberto Bethencourt
Affiliation:
Department Obstetrics and Gynaecology, Universidad de La Laguna. Reproduction Unit, Hospital Universitario de Canarias, Ctra. Ofra S/N La Cuesta, 38320La Laguna, Santa Cruz de Tenerife
*
Address for correspondence: Rubí Rodríguez Díaz. Unidad de Reproducción Humana. Hospital Universitario de Canarias (CHUC), Área Obstetricia y Ginecología, Universidad de La Laguna (ULL), Tenerife, Spain. Tel: +34 677 021 762. E-mail: rubrod@ull.edu.es
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Summary

HLA-G expression has been detected in early preimplantation embryos and it has been postulated that a relationship between embryonic expression of this factor and successful pregnancy may exist. Forty-six patients were prospectively selected from our centre ‘Unidad de Reproducción Humana, Hospital Universitario de Canarias’ for conducting this study. In all cases, metaphase II (MII) oocytes were fertilized using intracytoplasmic sperm injection 2–4 h after retrieval. Embryos were cultured individually in 20 µl droplets of G-1 medium (VitroLife) under oil at 37°C and a 6% CO2 environment. Fertilization was assessed at 18 h postinsemination and all oocytes fertilized were passed into a new culture plaque individually in 300 µl culture medium until day 3 of culture. The culture medium was examined for the expression and secretion of sHLA-G with a sandwich enzyme-linked immunosorbent assay kit (BioVendor, Heidelberg, Germany) according to the manufacturer’s instructions. We found statistical significance between higher levels of sHLA-G secretion and pregnancy rate. When both groups were compared there was no difference in embryo quality of transferred embryos, but a significant difference in the number of oocytes and the embryo quality of the cohort existed that was greater in the pregnant group. A standardized sHLA-G assay with a specifically defined range and standard units provides a non-invasive method to identify the most competent embryos for transfer.

Keywords

Embryo cohortEmbryo transferIVF/ICSI outcomePregnancySECRETOMICSSoluble HLA-G
Type
Research Article
Information
Zygote , Volume 27 , Issue 2 , April 2019 , pp. 78 - 81
Copyright
© Cambridge University Press 2019 

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