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Environment and medium volume influence in vitro fertilisation of pig oocytes

Published online by Cambridge University Press:  26 September 2008

P. Coy ,
E. Martínez ,
S. Ruiz ,
J.M. Vázquez ,
J. Roca  and
J. Gadea
P. Coy*
Affiliation:
University of Murcia, Murcia, Spain
E. Martínez
Affiliation:
University of Murcia, Murcia, Spain
S. Ruiz
Affiliation:
University of Murcia, Murcia, Spain
J.M. Vázquez
Affiliation:
University of Murcia, Murcia, Spain
J. Roca
Affiliation:
University of Murcia, Murcia, Spain
J. Gadea
Affiliation:
University of Murcia, Murcia, Spain
*
P. Coy, Departmento de Biologia Animal (Fisiología Animal), Facultad de Veterinaria, Campus de Espinardo, Universidad de Murcia, Aptdo. 4021, Murcia E-30071, Spain. Fax: 34-68 305101
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Summary

Oviductal oocytes (n = 795) were obtained from ovulation-induced prepubertal gilts. In the first experiment, different parameters related to in vitro fertilisation (IVF) were compared in the presence and absence of cumulus matrix (which is shed with the oocytes at ovulation.) The results show that the presence of this matrix is beneficial because the rates of fertilisation (69%) (number of monospermic oocytes/total number of healthy mature oocytes; 42%), and the median number of spermatozoa per oocyte (1.52±0.06), were improved with respect to those obtained in its absence (54%, 22% and 2.33 ± 0.08, respectively; p<0.01). In the second experiment the effect of two different volumes of co-incubation medium (2 ml and 0.4 ml) on the same parameters of porcine IVF were compared. No significant differences between volumes were observed, except in the mean number of spermtozoa per oocyte and the percentage of dispermic oocytes.

Keywords

CumulusIn vitro fertilisationMediumvolumeporine
Type
Review Article
Information
Zygote , Volume 1 , Issue 3 , August 1993 , pp. 209 - 213
Copyright
Copyright © Cambridge University Press 1993

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References

Barros, C. & Yanagimachi, R. (1971). Induction of zona reaction in golden hamster eggs by cortical granule material. Nature 233, 268–9.CrossRefGoogle ScholarPubMed
Barros, C. & Yanagimachi, R. (1972). Polyspermy-preventing mechsnisms in the golden hamster egg. J. Exp. Zool. 180, 251–66.CrossRefGoogle ScholarPubMed
Braden, A.W.H. (1962). Spermatozoon penetration and fertilization in the mouse. Symp. Genet. Biol. Ital. (Pavia) 9, 1–8.Google Scholar
Brown, C.R. & Cheng, W.T.K. (1986). Changes in composition of the porcine zona pellucida during development of the oocyte to the 2- to 4-cell embryo. J. Embryo. Exp. Morphol. 92, 183–91.Google Scholar
Chang, M.C. (1952). Fertilizabiity of rabbit ova and the effects of temperature in vitro on their subsequent fertilization and activation in vivo. J. Exp. Zool. 121, 351–81.CrossRefGoogle Scholar
Chang, M.C., Hanada, A. & Hunt, D.M. (1971). Fertilization of denuded rabbit eggs in vitro by sperm recovered from the uterus or vagina. Nature 232, 343–4.CrossRefGoogle ScholarPubMed
Cheng, W.T.K., Moor, R.M. & Polge, C. (1986). In vitro fertilization of pig and sheep oocytes matured in vivo and in vitro. Theriogenology 25, 146.CrossRefGoogle Scholar
Coy, P. (1992). Fecundación in vitro en la especie porcina: influencia de diferentes condiciones de cocultivo. Murcia: Secretariado de Publicaciones Universidad de Murcia. ISBN: 84-7684-277-5.Google Scholar
Coy, P., Martínez, E., Ruiz, S., Vázquez, J.M., Roca, J., Matas, C., Pellicer, M.T. (1993 a). In vitro fertilization of pig oocytes after different coincubation intervals. Theriogenology. 39, 1201–1208CrossRefGoogle ScholarPubMed
Coy, P., Martínez, E., Ruiz, S., Vazquez, J.M., Roca, J. & Matas, C. (1993b). Sperm concentration influences fertilization and male pronuclear formation in vitro in pigs. Theriogenology (in press).CrossRefGoogle ScholarPubMed
Cross, P.C. & Brinster, R.L. (1970). In vitro development of mouse oocytes. Biol. Reprod. 3, 298–307.CrossRefGoogle ScholarPubMed
Fraser, L.R., Danderkar, P.V. & Vaidya, R.A. (1971).In vitro fertilization of tubal rabbit ova partially or totally denuded of follicular cells. Biol. Reprod. 4, 229–33.CrossRefGoogle ScholarPubMed
Fukui, Y. & Ono, H. (1989). Effects of sera, hormones and granulosa cells added to culture medium for in vitro maturation, fertilization, cleavage and development of bovine oocytes, J. Reprod. Fert. 86, 501–6.CrossRefGoogle ScholarPubMed
Gwatkin, R.B.L., Andersen, O.F. & Hutchison, C.F. (1972).Capacitation of hamster spermatozoa in vitro; the role of cumulus components, J. Reprod. Fert. 30, 389–94.CrossRefGoogle ScholarPubMed
Hunter, R.H.F. (1991). Oviduct function in pigs, with particular reference to the pathological condition of polyspermy, Mol. Reprod. Dev. 29, 385–91.CrossRefGoogle Scholar
Kikuchi, K., Nagai, T., Motlik, J. (1991).Effect of follicle cells on in vitro fertilization of pig follicular oocytes. Theriogenology 35, 225.CrossRefGoogle Scholar
Martín, S., Perez, C. & Alias, E. (1983). Fertility results by using boar semen diluted 1:10 after 5 days' storage. In: 34 Reunión Anual de FEZ, Madrid. vol. 2, p. 755.Google Scholar
Mattioli, M., Galeati, C. & Seren, E. (1988).Effect of follicle somatic cells during pig oocyte maturation on egg penetrability and male pronucleus formation, Gamete Res. 20, 177–83.CrossRefGoogle ScholarPubMed
Mattioli, M., Bacci, M.L., Galeati, G. & Seren, E. (1989).Developmental competence of pig oocytes matured and fertilized in vitro, Theriogenology 31, 1201–7.CrossRefGoogle ScholarPubMed
Miyamoto, H., Chang, M.C. (1972). Fertilization in vitro of mouse and hamster eggs after the removal of follicular cells, J. Reprod. Fert. 30, 309–12.CrossRefGoogle ScholarPubMed
Nagai, T. & Moor, R.M. (1990). Effect of oviduct cells on the incidence of polyspermy in pig eggs fertilized in vitro, Mol. Reprod. Dev. 26, 377–82.CrossRefGoogle ScholarPubMed
Niwa, K. & Chang, M.C. (1974). Various conditions for the fertilization of rat eggs in vitro. Biol. Reprod. 11, 463–9.CrossRefGoogle ScholarPubMed
Park, C.S. & Pursel, V.G. (1991).In vitro fertilization of pig ova. J. Anim. Sci. 69, 404.Google Scholar
Sato, E., Miyamoto, H. & Koide, S.S.(1990).Glycosaminoglycans in porcine follicular fluid promoting viability of oocytes in culture, Mol. Reprod. Dev. 26, 391–7.CrossRefGoogle ScholarPubMed
Shioya, Y., Kuwayama, M.Fukushima, M., Iwasaki, S. & Hanada, A. (1988). In vitro fertilization and cleavage capability of bovine follicular oocytes classified by cumulus cells and matured in vitro. Theriogenology 30, 489–96.CrossRefGoogle ScholarPubMed
Vázquez, J.M. (1991). Fecundacion in vitro en la especie porcina: andlisis de la capacitacion y reacción acrosómica. Murcia: Secretariado de Publicaciones Universidad de Murcia. ISBN: 84-7684-240-6.Google Scholar
Verhage, H.G. & Fazleabas, A.T. (1990). Steroid-dependent oviduct secretions in the primate. In: Biology of the Mammalian Oviduct, Croxato, H.Santiago.Google Scholar
Yanagimachi, R. (1981). Mechanisms of fertilization in mammals. In:L., Mastroianni Jr. & J.D., Biggers (eds.):Fertilization and Embryonic Development In vitro,Mastroianni, L. Jr & Biggers, J.D.. pp.81–182. New York: Plenum Press.CrossRefGoogle Scholar
Yanagimachi, R.(1988). Mammalian fertilization. In:The Physiology of Reproduction, ed. Knobil, E. & Neill, J., 135–85, New York:Raven Press.Google Scholar
Yang, C.H., Yanagimachi, R. (1989). Differences between mature ovarian and oviductal oocytes: a study using the golden hamster, Hum. Reprod. 4 (1), 63–71.CrossRefGoogle ScholarPubMed