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Evaluating human sperm DNA integrity: relationship between 8-hydroxydeoxyguanosine quantification and the sperm chromatin structure assay

Published online by Cambridge University Press:  08 March 2004

Isabelle Oger ,
Christelle Da Cruz ,
Gilles Panteix  and
Yves Menezo
Isabelle Oger
Affiliation:
Institut Rhonalpin (IRH) Laboratoire Marcel Merieux, 1 Rue Laborde, 69500 Bron, France.
Christelle Da Cruz
Affiliation:
Laboratoire Marcel Mérieux, Avenue Tony Garnier, 69357 Lyon Cedex 07, France.
Gilles Panteix
Affiliation:
Laboratoire Marcel Mérieux, Avenue Tony Garnier, 69357 Lyon Cedex 07, France.
Yves Menezo
Affiliation:
Institut Rhonalpin (IRH) Laboratoire Marcel Merieux, 1 Rue Laborde, 69500 Bron, France.
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Abstract

In our work, we have used 8-hydroxy-deoxyguanosine (8-OH-dG), one of the major oxidative products of sperm DNA, in a population of patients consulting for infertility. We found an inverse relationship between sperm concentration and the log of the ratio of 8-OH-dG to dG (P<0.01). On the same patients' sperm samples, the sperm chromatin structure assay (SCSA) was performed. An inverse relationship was observed between the DNA fragmentation index and sperm concentration (P<0.001). There was also a positive relationship between SCSA and log 8-OH-dG/dG. This indicates that DNA fragmentation measured by the SCSA originates in part from oxidative products. In a few patients, antioxidant treatment decreased the DNA fragmentation index below the threshold of 30% that is crucial for subfertility.

Keywords

Sperm DNA fragmentationReactive oxygen species8-OxodeoxyguanosineSperm chromatin structure assay
Type
Research Article
Information
Zygote , Volume 11 , Issue 4 , November 2003 , pp. 367 - 371
Copyright
2003 Cambridge University Press

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