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Expression levels of mRNA for insulin-like growth factors 1 and 2, IGF receptors and IGF binding proteins in in vivo and in vitro grown bovine follicles

Published online by Cambridge University Press:  09 May 2013

Emanuela L. Rebouças ,
José J.N. Costa ,
Maria J. Passos ,
Anderson W.B. Silva ,
Rodrigo O.D.S. Rossi ,
Robert van den Hurk  and
José R.V. Silva
Emanuela L. Rebouças
Affiliation:
Biotechnology Nucleus of Sobral - NUBIS, Federal University of Ceara, Sobral, CE, Brazil
José J.N. Costa
Affiliation:
Biotechnology Nucleus of Sobral - NUBIS, Federal University of Ceara, Sobral, CE, Brazil
Maria J. Passos
Affiliation:
Biotechnology Nucleus of Sobral - NUBIS, Federal University of Ceara, Sobral, CE, Brazil
Anderson W.B. Silva
Affiliation:
Biotechnology Nucleus of Sobral - NUBIS, Federal University of Ceara, Sobral, CE, Brazil
Rodrigo O.D.S. Rossi
Affiliation:
Biotechnology Nucleus of Sobral - NUBIS, Federal University of Ceara, Sobral, CE, Brazil
Robert van den Hurk
Affiliation:
Department of Pathobiology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands
José R.V. Silva*
Affiliation:
Biotechnology Nucleus of Sobral–NUBIS, Federal University of Ceara, Rua Comandante Maurocélio Rocha Pontes 100, CEP 62042–280, Sobral, CE, Brazil.
*
All correspondence to: José R.V. Silva. Biotechnology Nucleus of Sobral–NUBIS, Federal University of Ceara, Rua Comandante Maurocélio Rocha Pontes 100, CEP 62042–280, Sobral, CE, Brazil. Tel:/Fax: +55 88 36132603. e-mail: jrvsilva@ufc.br
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Summary

This study investigated mRNA levels for insulin-like growth factors (IGFs) IGF1 (IGF-I) and IGF2 (IGF-II), IGF receptors (IGF1R and IGF2R), and binding proteins (IGFBP-1, IGFBP-2. IGFBP-3, IGFBP-4, IGFBP-5 and IGFBP-6) in bovine follicles of 0.2, 0.5 or 1.0 mm in diameter. mRNA expression levels in in vitro cultured follicles that reached approximately 0.5 mm were compared with that of in vivo grown follicles. IGF1R and IGF2R expression levels in 0.5 mm in vivo follicles were higher than in 1.0 or 0.2 mm follicles, respectively. IGFBP-1, IGFBP-2. IGFBP-3, IGFBP-4, IGFBP-5 and IGFBP-6 showed variable expression in the follicular size classes analyzed. In vitro grown follicles had significantly reduced expression levels for IGF1, IGF1R, IGFBP-3, IGFBP-5 and IGFBP-6 mRNA when compared with 0.2 mm follicles, but, when compared with in vivo grown follicles (0.5 mm), only IGFBP-1, IGFBP-2, IGFBP-3 and IGFBP-6 showed a reduction in their expression. In conclusion, IGFs, their receptors and IGFBPs showed variable expression of mRNA levels in the follicular size classes analyzed.

Keywords

BovineHousekeeping genesIGFmRNAOvarian follicles
Type
Research Article
Information
Zygote , Volume 22 , Issue 4 , November 2014 , pp. 521 - 532
Copyright
Copyright © Cambridge University Press 2013 

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