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Expression of c-fos and c-jun proto-oncogenes by ovine preimplantation embryos

Published online by Cambridge University Press:  26 September 2008

Bin Wu
Bin Wu
Affiliation:
Department of Animals and Veterinary Science, Northwestern Agricultural University, Yangling, Shaanxi 712100, People's Republic of China
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Summary

The c-fos and c-jun proto-oncogenes are involved in the regulation of gene expression, cell proliferation, differentiation and tumorigenesis. Embryogenesis, like tumorigenesis, involves dramatic cell growth, cleavage and differentiation processes. Activation of the c-fos and c-jun proto-oncogenes in sheep conceptuses during the period of rapid growth and elongation was examined using reverse transcription and polymerase chain reaction (RT-PCR). The specificity of PCR products was determined by Southern blot hybridisation analysis with a non-radioactive DNA probe. A band corresponding to a 507 bp fragment (predicted amplified c-fos gene cDNA product) was observed in 3 of 9 day-13, 1 of 4 day-14 and 1 of 2 day-16 embryos. Meanwhile, a 400 bp c-jun transcript was aslo detected in 1 or 2 day-12, 3 of 9 day-13 and 2 of 2 day-16 embroyos. These results suggest that mRNA transcripts of c-fos and c-jun proto-oncogenes were specifically expressed during the period of ovine embryonic elongation and may have a possible role in preimplantation embryonic development of sheep.

Keywords

ElongationEmbryomRNAcRNAcDNART-PCR
Type
Article
Information
Zygote , Volume 4 , Issue 3 , August 1996 , pp. 211 - 217
Copyright
Copyright © Cambridge University Press 1996

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