Hostname: page-component-78c5997874-4rdpn Total loading time: 0 Render date: 2024-11-10T14:37:17.678Z Has data issue: false hasContentIssue false
  • English
  • Français

Expression of c-fos and c-jun proto-oncogenes by ovine preimplantation embryos

Published online by Cambridge University Press:  26 September 2008

Bin Wu
Bin Wu
Affiliation:
Department of Animals and Veterinary Science, Northwestern Agricultural University, Yangling, Shaanxi 712100, People's Republic of China
Get access Rights & Permissions [Opens in a new window]

Summary

The c-fos and c-jun proto-oncogenes are involved in the regulation of gene expression, cell proliferation, differentiation and tumorigenesis. Embryogenesis, like tumorigenesis, involves dramatic cell growth, cleavage and differentiation processes. Activation of the c-fos and c-jun proto-oncogenes in sheep conceptuses during the period of rapid growth and elongation was examined using reverse transcription and polymerase chain reaction (RT-PCR). The specificity of PCR products was determined by Southern blot hybridisation analysis with a non-radioactive DNA probe. A band corresponding to a 507 bp fragment (predicted amplified c-fos gene cDNA product) was observed in 3 of 9 day-13, 1 of 4 day-14 and 1 of 2 day-16 embryos. Meanwhile, a 400 bp c-jun transcript was aslo detected in 1 or 2 day-12, 3 of 9 day-13 and 2 of 2 day-16 embroyos. These results suggest that mRNA transcripts of c-fos and c-jun proto-oncogenes were specifically expressed during the period of ovine embryonic elongation and may have a possible role in preimplantation embryonic development of sheep.

Keywords

ElongationEmbryomRNAcRNAcDNART-PCR
Type
Article
Information
Zygote , Volume 4 , Issue 3 , August 1996 , pp. 211 - 217
Copyright
Copyright © Cambridge University Press 1996

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Adamson, E.D.. (1987). Review article: expression of proto-oncogenes in the placenta. Placenta 8, 449–66.CrossRefGoogle ScholarPubMed
Ahmad, K. & Naz, R.K.. (1993). Presence and possible role of c-ras and nuclear (c-fos and c-jun) proto-oncogene products in preimplantation embryonic development in mice. Mol. Reprod. Dev. 36, 297–306.CrossRefGoogle ScholarPubMed
Angel, P., Allegretto, E.A., Okino, S.T., Hattori, K., Boyle, W.J., Hunter, T. & Karin, M.. (1988). Oncogene jun encodes a sequence-specific trans-activator similar to AP-1. Nature 332, 166–71.CrossRefGoogle ScholarPubMed
Chiu, R., Boyle, W.J., Meek, J., Smeal, T., Hunter, T. & Karin, M.. (1988). The c-Fos protein interacts with c-Jun/AP-1 to stimulate transcription of AP-1 responsive genes. Cell 54, 541–52.CrossRefGoogle ScholarPubMed
Collart, M.A., Tourkine, N., Belin, D., Vassalli, P., Jeanteur, P. & Blanchard, J.M.. (1991). c-fos gene transcription in murine macrophages is modulated by a calcium-dependent block to elongation in intron 1. Mol. Cell Biol 11, 2828–31.Google ScholarPubMed
Curran, T. & Franza, R. Jr., (1988). Fos and Jun: the AP-1 connection (Minireview). Cell 55,395–7.CrossRefGoogle Scholar
Davis, G.L., Dibner, D.M. & Battey, F.J.. (1986). Plasmid DNA preparation. In Basic Methods in Molecular Biology, pp. 89–104. Amsterdam: Elsevier.Google Scholar
Distel, R.J. & Spiegelman, B.M.. (1990). Protooncogene c-fos as a transcription factor. Adv. Cancer Res. 55, 37–55.CrossRefGoogle ScholarPubMed
Dubois, D.H., Simmen, R.C.M.., Fliss, A., Smith, I.C. & Bazer, F.W.. (1993). Expression of c-fos in porcine endometrium during the estrous cycle and early pregnancy. Biol. Reprod 49, 943–50.CrossRefGoogle ScholarPubMed
Eisenman, R.H.. (1989). Nuclear oncogenes. In Oncogenes and Molecular Origins of Cancer, ed. Weinberg, R.A., 193–205. New York: Cold Spring Harbor laboratory Press.Google Scholar
Franza, B.R. Jr, Rauscher, F.J. III., Josephs, S.F. & Curran, T.. (1988). The Fos complex and Fos-related antigens recognize sequence elements that contain AP-1 binding sites. Science 239, 1150–3.CrossRefGoogle ScholarPubMed
Gonda, T.J. & Metcalf, D.. (1984). Expression of myb, myc and fos proto-oncogenes during the differentiation of a murine myeloid leukaemia. Nature 310, 249–51.CrossRefGoogle ScholarPubMed
Kiessling, A.A. & Cooper, G.M.. (1989). The expression of oncongenes in mammalian embryogenesis. In Growth Factor in mammalian Development, ed. Rosenbium, I.Y. & Heyner, S., pp. 167–74. GRC Press.Google Scholar
Kimelman, D. & Kirschner, M.. (1987). Synergistic induction of mesoderm by FGF and TGF-β and the identification of an mRNA coding for FGF in the early Xenopus embryo. Cell 51, 869–77.CrossRefGoogle ScholarPubMed
Martin, G.R. (1980). Teratocarcinomas and mammalian embryogenesis. Science 209, 768–76.CrossRefGoogle ScholarPubMed
Müller, R.. (1986). Cellular and viral fos genes: structure, regulation of expression and biological properties of their encoded products. Biochim. Biophys. Acta 823, 207–25.Google ScholarPubMed
Müller, R. & Wagner, E.. (1984). Differentiation of F9 teratocarcinoma stem cells after transfer of c-fos proto-oncogenes. Nature 311, 438–42.CrossRefGoogle ScholarPubMed
Müller, R., Slamon, D.J., Tremblay, J.M., Cline, M.J. & Verma, I.M.. (1982). Differential expression of cellular oncogenes during pre- and postnatal development of the mouse. Nature 299, 640–4.CrossRefGoogle ScholarPubMed
Müller, R., Muller, D., Verrier, B., Bravo, R. & Herbst, H.. (1986). Evidence that expression of c-fos protein in amnion cell is regulated by external signals. EMBO J. 5, 311–16.CrossRefGoogle ScholarPubMed
Pal, S.K., Crowell, R., Kiessling, A.A. & Cooper, G.M.. (1993). Expression of proto-oncogenes in mouse eggs and preimplantation embryos. Mol. Reprod. Dev. 35, 8–15.CrossRefGoogle ScholarPubMed
Risse, G., Neuberg, M., Hunter, J.B., Verner, B. & Muller, R..(1990). Products of the fos and jun proto-oncogenes bind cooperatively to the AP-1 DNA recognition sequence. Environ. Health Perspect 88, 133–9.CrossRefGoogle Scholar
Ryder, K. & Nathans, D.. (1988). Introduction of proto-oncogene c-jun by serum growth factor. Proc. Natl. Acad. Sci. USA 85, 8464–7.CrossRefGoogle Scholar
Sassone-Corsi, P., Sisson, J.C. & Verma, I.M.. (1988a). Transcriptional auto-regulation of the proto-oncogene fos. Nature 334, 314–19.CrossRefGoogle Scholar
Sassone-Corsi, P., Lamph, W.W., Kamps, M. & Verma, I.M.. (1988b). fos-associated cellular P39 is related to nuclear transcription factor AP-1. Cell 54, 553–60.CrossRefGoogle ScholarPubMed
Sassone-Corsi, P., Ransone, L.J., Lamph, W.W. & Verma, I.M.. (1988c). Direct interaction between fos and jun nuclear oncoproteins: role of the [leucine zipper] domain. Nature 336, 692–5.CrossRefGoogle Scholar
Schonthal, A., Herrlich, P., Rahmsodorf, H.J. & Ponta, H.. (1988). Requirement for fos gene expression in the transcriptional activation of collaguease by other oncogenes and phorbol esters. Cell 54, 325–34.CrossRefGoogle ScholarPubMed
Schutte, J., Viallet, J., Nau, M., Segal, S., Fedorko, J. & Minna, J.. (1989). Jun-B inhibits and c-fos stimulates the transforming and trans-activating activities of c-jun. Cell 59, 987–97.CrossRefGoogle ScholarPubMed
Straaten, F.V., Müller, R., Curran, T., Beveren, C.V. & Verma, I.M.. (1983). Complete nucleotide sequence of human c-onc gene: deduced amino acid sequence of the human c-fos protein. Proc. Natl. Acad. Sci. USA 80, 3183–7.CrossRefGoogle ScholarPubMed
Ullrich, A., Schlessinger, J. & Waterfield, M.D.. (1984). Close similarity of epidermal growth factor receptor and v-erb-B oncogene protein sequences. Nature 307, 521.Google Scholar
Van Blerkom, J. (1981). Intrinsic and extrinsic patterns of molecular differentiation during oogenesis, embryogenesis and organogenesis in mammals. In Cellular and Molecular Aspects of Implantation, ed. Glasser, S.R. & Bullock, D.W.. New York: Plenum Press.Google Scholar
Vogt, P.K., Bos, T.J. & Doolittle, R.F.. (1987). Homology between the DNA-binding domain of the GCN4-regulatory protein of yeast and the carboxyl-terminal region of a protein coded for by the oncogene jun. Proc. Natl. Acad. Sci. USA 84, 3316–19.CrossRefGoogle ScholarPubMed
Willingham, T., Shelton, M. & Thompson, P.. (1986). An assessment of reproductive wastage in sheep. Theriogenology 26, 179–88.CrossRefGoogle ScholarPubMed
Whyte, A. & Stewart, H.J.. (1989). Expression of the proto-oncogene fos (c-fos) by preimplantation blastocysts of the pig. Development 105, 651–6.CrossRefGoogle ScholarPubMed