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Factors related to follicular oxidative stress in intracytoplasmic sperm injection cycles and its effects on granulosa cells

Published online by Cambridge University Press:  28 September 2020

Seda Karabulut Open the ORCID record for Seda Karabulut [Opens in a new window] ,
Oya Korkmaz ,
Pelin Kutlu  and
Ilknur Keskin
Seda Karabulut*
Affiliation:
Istanbul Medipol University, International School of Medicine, Histology and Embryology Department, Istanbul, Turkey Istanbul Medipol University, REMER Center, Istanbul, Turkey
Oya Korkmaz
Affiliation:
Istanbul Medipol University, International School of Medicine, Histology and Embryology Department, Istanbul, Turkey Istanbul Medipol University, REMER Center, Istanbul, Turkey
Pelin Kutlu
Affiliation:
Medicana Çamlıca Hospital, IVF Center, Istanbul, Turkey
Ilknur Keskin
Affiliation:
Istanbul Medipol University, International School of Medicine, Histology and Embryology Department, Istanbul, Turkey Istanbul Medipol University, REMER Center, Istanbul, Turkey
*
Author for correspondence: Seda Karabulut. Istanbul Medipol University, International School of Medicine, Department of Histology and Embryology, Kavacık Neighborhood, 19 Ekinciler Street, Beykoz, Istanbul, Turkey. Tel: +90 532 273 98 64. E-mail: sedakarabulut@medipol.edu.tr
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Summary

The aim of the present study was to investigate several common conditions that may potentially be correlated with follicular oxidative status during an intracytoplasmic sperm injection (ICSI) cycle and that include the serum oestrogen level on the day of oocyte pick-up, maternal age and pregnancy outcome. Patients that were enrolled in the study were classified randomly into three groups using their numerical order. The first group were classified based on maternal age (<35 and ≥35 years) (n = 398), the second group on the serum oestradiol (E2) level on the day of human chorionic gonadotropin (hCG) administration (levels >90th percentile and ≤ 90th percentile) (n = 491) and the third group on pregnancy outcome (positive/negative) (n = 376). The groups were matched for the other variables (stimulation protocol, dose of gonadotropin, duration of stimulation, antral follicle count, body mass index, basal follicle stimulating hormone (FSH), and E2 levels and day of hCG trigger) to prevent the possible contribution of those parameters to the results. Each group was matched for other variables (stimulation protocol, dose of gonadotrophin, duration of stimulation, antral follicle count, body mass index, basal FSH and E2 levels and day of hCG trigger) that may have affected the outcome, except for the parameter under investigation. Maternal age (P = 0.044,168 r = 0.418), oestrogen level on day of hCG administration (P = 0.001, r = 0.436) and pregnancy outcome (AUC = 0.65, P = 0.071) were found to be correlated with follicular oxidative status. The results obtained will help us to shield patients from possible situations that may cause oxidative stress and therefore adverse outcomes of an ICSI cycle.

Keywords

DNAFollicular fluidIntracytoplasmic sperm injectionMural granulosa cellsOxidative stress
Type
Research Article
Information
Zygote , Volume 29 , Issue 1 , February 2021 , pp. 59 - 65
Copyright
© The Author(s), 2020. Published by Cambridge University Press

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