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Gonadotropin suppression of apoptosis in follicle somatic cells

Published online by Cambridge University Press:  26 September 2008

G. Galeati ,
M. Forni  and
M. Spinaci
G. Galeati*
Affiliation:
Dipartimento di Morfofisiologia Veterinaria e Produzioni Animali, Ozzano Emilia, Bologna, Italy.
M. Forni
Affiliation:
Dipartimento di Morfofisiologia Veterinaria e Produzioni Animali, Ozzano Emilia, Bologna, Italy.
M. Spinaci
Affiliation:
Dipartimento di Morfofisiologia Veterinaria e Produzioni Animali, Ozzano Emilia, Bologna, Italy.
*
G. Galeati, Dept di Morfofisiologia Veterinaria e Produzioni Animali, Via Tolara di Sopra 50, 40064, Ozzano Emilia-Bologna, Italy.
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Extract

In each oestrous cycle only a limited number of follicles are selected for ovulation whereas the remaining majority undergo atresia. The earliest and most prominent feature of atresia is the death of granulosa cells. Recent biochemical evidence has demonstrated that granulosa cell death during follicular atresia in swine (Tilly et al., 1992), bovine (Jolly et al., 1994) and rodent (Tilly et al., 1991) ovaries occurs by apoptosis, a process whereby cells die in a controlled manner. A biochemical event considered to be characteristic of apoptotic cell death is the intranucleosomal cleavage of genomic DNA into fragments 180–200 bp in size, which separate into a distinctive ladder-like pattern on agarose gel electrophoresis. Detection of this pattern of oligonucleosomes in DNA provides a marker of apoptotic cell death.

Type
Article
Information
Zygote , Volume 4 , Issue 4 , November 1996 , pp. 299 - 303
Copyright
Copyright © Cambridge University Press 1996

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References

Baranao, J.L.S. & Hammond, J.M. (1984). Comparative effects of insulin and insulin-like growth factors on DNA synthesis and differentiation of porcine granulosa cells. Biochem. Biophys. Res. Commun. 24, 484–90.Google Scholar
Chang, S.C.S., Jones, J.D., Elefson, R.D. & Ryan, R.J. (1976). The porcine ovarian follicle. I. selected chemical analyis of follicular fluid at different developmental stages. Biol. Reprod. 15, 321–8.Google Scholar
Chun, S., Billig, H., Tilly, J.L., Furuta, I., Tsafriri, A. & Hsueh, A.J.W.. (1994). Gonadotropin suppression of apoptosis in cultured preovulatory follicles: mediatory role of endogenous insulin-like growth factor I. Endocrinology 135, 1845–53.Google Scholar
Grimes, R.W., Samaras, S.E., Barber, J.A., Shimasaki, S., Ling, N. & Hammond, J.M. (1992). Ginadotropin and cAMP modulation of IGF binding protein production in ovarian granulosa cells. Am. J. Physiol. 262, E497–503.Google Scholar
Henderson, K.M., Kieboom, L.E., McNatty, K.P., Lun, S. & Heath, D. (1985). Gonadotropin stimulated cyclic AMP production by granulosa cells from Booroola × Romney ewes with and without a fecundity gene. J. Reprod. Fertil. 75, 111–20.Google Scholar
Hsu, C.J. & Hammond, J.M. (1987). Gonadotropins and estradiol stimulate immunoreactive insulin-like growth factor-I production by porcine granulosa cells in vitro. Endocrinology 120, 198–207.CrossRefGoogle ScholarPubMed
Hughes, F.M. Jr & Gorospe, W.C. (1991). Biochemical identification of apoptosis (programmed cell death) in granulosa cells: evidence for a potential mechanism underlying follicular atresia. Endocrinology 129, 2415–22.Google Scholar
Jolly, P.D., Tisdall, D.J., Heath, D.A., Lun, S. & McNatty, K.P. (1994). Apoptosis in bovine granulosa cells in relation to steroid synthesis, cyclic adenosine 3',5'-monophosphate response to follicle-stimulating hormone and luteininzing hormone, and follicular atresia. Biol. Reprod. 51, 934–44.Google Scholar
Legrand, A.B., Narayanan, T.K., Ryan, U.S., Arostam, R.S. & Catravas, J.D. (1989). Modulation of adenylate cyclase activity in cultured bovine pulmonary arterial endothelial cells: effects of adenosine and derivates. Biochem. Pharmacol. 38, 423–30.Google Scholar
Mattioli, M., Galeati, G. & Seren, E. (1988). Effects of follicle somatic cells during pig oocyte maturation on egg penetrability and male pronucleus formation. Gamete Res. 20, 177–83.CrossRefGoogle ScholarPubMed
Mattioli, M., Bacci, M.L., Galeati, G. & Seren, E. (1991). Effects of LH and FSH on maturation of pig oocytes in vitro. Theriogenology 36, 95–105.CrossRefGoogle ScholarPubMed
Mattioli, M., Galeati, G., Barboni, B. & Seren, E. (1994). Concentration of cyclic AMP during the maturation of pig oocyte in vivo and in vitro. J. Reprod. Fertil. 100, 403–9.CrossRefGoogle ScholarPubMed
Muller, G. & Bandlow, W. (1994). Lipolytic membrane release of two phosphatidylinositol-anchored cAMP receptor proteins in yeast alters their ligand-binding parameters. Arch. Biochem. Biophys. 308, 504–14.CrossRefGoogle ScholarPubMed
Tilly, J.L. (1993). Ovarian follicular atresia: a model to study the mechanisms of physiological cell death. Endocr. J. 1, 67–72.Google Scholar
Tilly, J.L., Kowalski, K.I., Johnson, A.L. & Hsueh, A.J.W. (1991). Involvement of apoptosis in ovarian follicular atresia and post-ovulatory regression. Endocrinology 129, 2799–801.CrossRefGoogle Scholar
Tilly, J.L., Kowalski, K.I., Schomberg, D.W. & Hsueh, A.J.W.. (1992). Apoptosis in atretic ovarian follicles is associated with selective decreases in messenger ribonucleic acid transcripts for gonadotropin receptors and cytochrome P450 aromatase. Endocrinology 131, 1670–6.CrossRefGoogle ScholarPubMed
Van-Haastert, P.J.. (1994). Intracellular adenosine 3',5'-phosphate fomation is essential for down-regulation of surface adenosine 3',5'-phosphate receptors in Dictytostelium. Biochem. J. 303, 539–45.Google Scholar
Veldhuis, J.D. & Furlanetto, R.W. (1985). Trophic actions of human sometomedin C/insulin-like growth factor I on ovarian cells: in vitro studies with swine granulosa cells. Endocrinology 116, 1235–42.Google Scholar