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Hormonal induction and semen characteristics of tambaqui Colossoma macropomum

Published online by Cambridge University Press:  05 January 2011

Alexandre Nizio Maria ,
Hymerson Costa Azevedo ,
Jadson Pinheiro Santos  and
Paulo César Falanghe Carneiro
Alexandre Nizio Maria
Affiliation:
Laboratório de Biotecnologia da Reprodução Animal, Embrapa Tabuleiros Costeiros, Av. Beira Mar 3250, Aracaju, SE, Brazil.
Hymerson Costa Azevedo
Affiliation:
Laboratório de Biotecnologia da Reprodução Animal, Embrapa Tabuleiros Costeiros, Av. Beira Mar 3250, Aracaju, SE, Brazil.
Jadson Pinheiro Santos
Affiliation:
Laboratório de Biotecnologia da Reprodução Animal, Embrapa Tabuleiros Costeiros, Av. Beira Mar 3250, Aracaju, SE, Brazil.
Paulo César Falanghe Carneiro*
Affiliation:
Laboratório de Biotecnologia da Reprodução Animal, Embrapa Tabuleiros Costeiros, Av. Beira Mar 3250, CEP 49025–040, Aracaju, SE, Brazil.
*
All correspondence to: P. C. F. Carneiro. Laboratório de Biotecnologia da Reprodução Animal, Embrapa Tabuleiros Costeiros, Av. Beira Mar 3250, CEP 49025–040, Aracaju, SE, Brazil. Tel: +55 79 4009 1350. Fax: +55 79 4009 1303. e-mail: paulo@cpatc.embrapa.br
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Summary

In the hatchery-bred tambaqui Colossoma macropomum, spontaneous semen release does not occur, and hand-stripping produces reduced semen volume. The goal of this work is to evaluate the effects of hormonal induction with carp pituitary extract (CPE) on both qualitative (visual aspect, pH, motility, viability and morphological abnormalities) and quantitative (volume, concentration and number of spermatozoa per ejaculate) traits of tambaqui semen. Eleven males were treated with CPE (induced), and 11 were left untreated as a control (non-induced). All analysed parameters except motility and percentage of viable spermatozoa presented significant differences (p < 0.05) between the induced and non-induced treatments. CPE induction resulted in a 25-fold increase in semen volume and a 10-fold increase in the number of spermatozoa collected. However, both sperm concentration and the frequency of sperm with morphological abnormalities (commonly detached heads or bent tails) were significantly lower in CPE-induced fish. The hormonal induction of tambaqui males with CPE is efficient and positively influences some qualitative and quantitative properties of semen. Additionally, semen collection via gentle abdominal massage occurs more readily in CPE-induced fish.

Keywords

Carp pituitary extractColossoma macropomumFishSemen qualitySperm morphology
Type
Research Article
Information
Zygote , Volume 20 , Issue 1 , February 2012 , pp. 39 - 43
Copyright
Copyright © Cambridge University Press 2011

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