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Hormonal induction of Brycon cephalus (Characiformes, Characidae) to spermiation using D-ala6, pro9net-mGnRH + metoclopramide

Published online by Cambridge University Press:  15 July 2015

Cristiane Bashiyo-Silva ,
Raphael da Silva Costa ,
Douglas de Castro Ribeiro ,
José Augusto Senhorini ,
Rosicleire Veríssimo-Silveira  and
Alexandre Ninhaus-Silveira
Cristiane Bashiyo-Silva
Affiliation:
Laboratório de Ictiologia Neotropical - LINEO, Depto de Biologia e Zootecnia, FEIS/UNESP – Universidade Estadual Paulista ‘Júlio de Mesquita Filho’, Rua Monção, 226 – Zona Norte 15385–000 – Ilha Solteira, São Paulo, Brazil.
Raphael da Silva Costa
Affiliation:
Laboratório de Ictiologia Neotropical - LINEO, Depto de Biologia e Zootecnia, FEIS/UNESP – Universidade Estadual Paulista ‘Júlio de Mesquita Filho’, Rua Monção, 226 – Zona Norte 15385–000 – Ilha Solteira, São Paulo, Brazil.
Douglas de Castro Ribeiro
Affiliation:
Laboratório de Ictiologia Neotropical - LINEO, Depto de Biologia e Zootecnia, FEIS/UNESP – Universidade Estadual Paulista ‘Júlio de Mesquita Filho’, Rua Monção, 226 – Zona Norte 15385–000 – Ilha Solteira, São Paulo, Brazil.
José Augusto Senhorini
Affiliation:
Centro Nacional de Pesquisa e Conservação de Peixes Continentais (CEPTA/ICMBio), Rodovia SP-201 (Pref. Euberto Nemésio Pereira de Godoy), Km 6.5, 13630–970 – Pirassununga, São Paulo, Brazil.
Rosicleire Veríssimo-Silveira
Affiliation:
Laboratório de Ictiologia Neotropical - LINEO, Depto de Biologia e Zootecnia, FEIS/UNESP – Universidade Estadual Paulista ‘Júlio de Mesquita Filho’, Rua Monção, 226 – Zona Norte 15385–000 – Ilha Solteira, São Paulo, Brazil.
Alexandre Ninhaus-Silveira*
Affiliation:
Department of Biology and Zootechny, FEIS/UNESP – State University of São Paulo ‘Júlio de Mesquita Filho’, Rua Monção, 226, Zona Norte, Ilha Solteira, SP, 15385–000, Brazil.
*
All correspondence to: Alexandre Ninhaus-Silveira. Department of Biology and Zootechny, FEIS/UNESP – State University of São Paulo ‘Júlio de Mesquita Filho’, Rua Monção, 226, Zona Norte, Ilha Solteira, SP, 15385–000, Brazil. Tel: +55 18-37431255. Fax: +55 18-37422140. E-mail: ninhaus@bio.feis.unesp.br
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Summary

This study aimed to establish a hormonal induction protocol for spermiation of Brycon cephalus males, using Ala6, Pro9Net-mGnRH + metoclopramide (Ovopel®). Thus, 20 males were used divided into three inductor treatments [⅓ pellet/kg (T1), ⅔ pellet/kg (T2) and 1⅓ pellet/kg (T3)] and one control group (CO), which only received physiological solution applications (0.9% NaCl). All treatments were applied in a single dose. For evaluation of the availability of the treatment, the following seminal parameters were analyzed: seminal volume, subjective spermatic motility, duration of motility, pH, osmolality and spermatic concentration. T3 showed the highest seminal volume (4.66 ± 1.52 ml), and was significantly different in comparison with T1 (2.0 ± 0.9 ml), T2 (3.5 ± 1.3 ml) and CO (2.3 ± 1.2 ml). In relation to spermatic motility, T2 and T3 showed significantly higher levels [5, (81–100%)]. However, T3 showed significantly lower average sperm motility duration than T1, T2 and CO (30 ± 7 s; 28 ± 6 s; 32 ± 8 s, respectively). With regard to the seminal parameters of spermatic concentration, pH and osmolality, no significant variation was verified among treatments. In conclusion, mGnRH + metoclopramide used for hormonal induction of B. cephalus reproduction does not induce changes related to spermatic concentration, pH and osmolality parameters of the seminal fluid and the most adequate doses among tested treatments were ⅔ pellet/kg live fish.

Keywords

Induced spawningNeotropical fishSemen qualitySeminal characteristics
Type
Research Article
Information
Zygote , Volume 24 , Issue 3 , June 2016 , pp. 319 - 325
Copyright
Copyright © Cambridge University Press 2015 

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References

Alexandre, J.S., Ninhaus-Silveira, A., Veríssimo-Silveira, R., Buzollo, H., Senhorini, J.A. & Chaguri, M.P. (2009). Structural analysis of the embryonic development in Brycon cephalus (Gunther, 1869). Zygote 18, 173–83.CrossRefGoogle ScholarPubMed
Andrade-Talmelli, E.F., Kavamoto, E.T. & Fenerich-Verani, N. (2001). Características seminais da piabanha, Brycon insignis (STEINDACHNER, 1876), após estimulação hormonal. Bol. Inst. Pesca 27, 149–54.Google Scholar
Bernardino, G., Senhorini, J.A., Fontes, N.A., Bock, C.L. & Mendonça, J.O.J. (1993). Propagação artificial do Matrinchã, Brycon cephalus (Guenther, 1869), (Teleostei: Characidae). Bol. Inst. Pesca 6, 1–9.Google Scholar
Caneppele, D., Honji, R.M., Hilsdorf, A.W.S. & Moreira, R.G. (2009). Induced spawning of the endangered Neotropical species Steindachneridion parahybae (Siluriformes: Pimelodidae). Neotrop. Ichthyol. 7, 759–62.CrossRefGoogle Scholar
Carneiro, P.C.F. & Mikos, J.D. (2008). Gonadotrofina coriônica humana e hormônio liberador de gonadotrofina como indutores da reprodução do jundiá. Acta Sci. Anim. 30, 345–50.Google Scholar
Carolsfeld, J., Godinho, H.P., Zaniboni-Filho, E. & Harvey, B.J. (2003). Cryopreservation of sperm in Brazilian migratory fish conservation. J. Fish Biol. 63, 472–89.Google Scholar
Cejko, B.I., Kowalski, R.K., Kucharczyk, D., Targonska, K., Krejszeff, S., Zarski, D. & Glogowski, J. (2010). Influence of the length of time after hormonal stimulation on selected parameters of milt of Leuciscus idus L. Aquac. Res. 41, 804–13.Google Scholar
Cejko, B.I., Targonska, K., Kowalski, R.K., Zarski, D., Sarosiek, B., Kucharczyk, D. & Glogowski, J. (2012a). The effectiveness of hormonal preparations (Ovopel, Ovaprim, LHRHa, hCG and CPE) in stimulating spermiation in dace Leuciscus leuciscus (L.). J. Appl. Ichthyol. 28, 873–7.Google Scholar
Cejko, B.I., Kowalski, R.K., Zarski, D., Dryl, K., Targonska, K., Chwaluczyk, R., Kucharczyk, D. & Glogowski, J. (2012b). The influence of the length of time after hormonal treatment with [(D-Ala6, Pro9 NEt)-mGnRH + metoclopramide] i.e. Ovopel on barbel Barbus barbus (L.) milt quality and quantity indicators. J. Appl. Ichthyol. 28, 249–53.CrossRefGoogle Scholar
Cejko, B.I., Zarski, D., Judycka, S., Kucharczyk, D., Sarosiek, B. & Kowalski, R.K. (2014). Effect of two commercial preparations containing different GnRH analogues with dopamine antagonists on barbel Barbus barbus (L.) sperm quantity and quality. Aquacult. Int. 22, 97–109.CrossRefGoogle Scholar
Crepaldi, D.V., Faria, P.M.C., Teixeira, E.A., Ribeiro, L.P., Costa, A.A.P., Melo, D.C., Cintra, A.P.R., Prado, A.S., Costa, F.A.A., Drumond, M.L., Lopes, V.E. & Moraes, V.E. (2006). Utilização de hormônios na reprodução induzida do surubim (Pseudoplatystoma spp.). Rev. Bras. Reprod. Anim. 30, 168–73.Google Scholar
Cruz-Casallas, P.E., Medina-Robles, V.M. & Velasco-Santamaria, Y.M. (2006). Evaluación de diferentes crioprotectores para la crioconservación de espermatozoides de yamú (Brycon amazonicus). Rev. Col. Cienc. Pec. 19, 152–9.Google Scholar
Donaldson, E.M. & Hunter, G.M. (1983). Induced final maturation, ovulation and spermiation. Fish Physiol. 9, 351–403.Google Scholar
Ferreira, A.A., Nuner, A.P.O., Luz, R.K., Tataje, D.A.R., Esquivel, J.R., Restrepo, J.B. (2001). Avaliação qualitativa e quantitativa do sêmen de jundiá, Rhamdia quelen . Bol. Inst. Pesca 27, 57–60.Google Scholar
Fogli da Silveira, W., Kavamoto, E.T., Cestarolli, M.A., Godinho, H.M., Ramos, S.M. & Silveira, A.N. (1990). Avaliação espermática, preservação criogênica e fertilidade do sêmen do pacu, Piaractus mesopotâmicus (Holmberg, 1887), proveniente de reprodução induzida. Bol. Inst. Pesca 17, 1–13.Google Scholar
Godinho, H.P. & Godinho, A.L. (1986). Induced spawning of the pacu, Colossoma mitrei (Berg1895), by hypophysation with crude carp pituitary extract. Aquaculture 55, 69–73.CrossRefGoogle Scholar
Honji, R.M, Mello, P.H., Araújo, B.C., Rodrigues-Filho, J.A., Hilsdorf, A.E.S. & Moreira, R.G. (2011). Influence of spawning procedure on gametes fertilization success in Salminus hilarii Valenciennes, 1850 (Teleostei: Characidae): implications for the conservation of this species. Neotrop. Ichthyol. 9, 363–70.Google Scholar
Howes, G. (1982). Review of the Genus Brycon (Teleostei, Characoidei) (Bulletin of the British Museum). UK: British Museum (Natural History).Google Scholar
Jamróz, M., Kucharczyk, D., Hakuc-Btazowska, A., Krejszeff, S., Kujawa, R., Kupren, K., Kwiatkowski, M., Targonska, K., Jarski, D., Cejko, B.I. & Glogowski, J. (2008). Comparing the effectiveness of Ovopel, ovaprim, and LH-RH analogue used in the controlled reproduction of ide, Leuciscus idus (L.). Arch. Polish Fish. 16, 363–70.Google Scholar
Kavamoto, E., Ferraz, E.M., Andrade-Talmelli, E.F., Meinardes-Pinto, C.S.R., Romagosa, E., Narahara, M.Y., Barnabe, V.H. & Campos, B.E.S. (1996). Estimulação da espermiação em curimbatá Prochilodus scrofa (Steindachner) através de aplicações de hCG (Ostheichthyes, Characiformes, Prochilodontidae). Rev. Bras. Zool. 13, 27–38.Google Scholar
Kayim, M., Bozkurt, Y. & Ogretmen, F. (2010). Comparing the effectiveness of Ovopel and carp pituitary extract (CPE) on artificial spawning of scaly carp (Cyprinus carpio). J. Animal and Veterinary Advances 9, 2589–92.Google Scholar
Krejszeff, S., Targonska, K., Zarski, D. & Kucharczyk, D. (2010). Artificial reproduction of two different spawn-forms of the chub. Reprod. Biol. 10, 67–74.CrossRefGoogle ScholarPubMed
Kucharczyk, D., Szczerbowski, A., Luczynski, M. J., Kujawa, R., Mamcarz, A., Wyszomirska, E., Szabó, T. & Ratajski, S. (2001). Artificial spawning of eurasian perch, Perca fluviatilis L. using Ovopel. Arch. Polish Fish. 9, 39–49.Google Scholar
Lenis, G.A., Restrepo, L.F., Rivera, J.C., Monsalve, F. & Cruz-Casallas, P.E. (2009). Reproducción inducida y producción de alevinos de Sabaleta Brycon henni: determinación del tiempo de latencia utilizando extracto de hipófisis de carpa. Rev. Colomb. Cienc. Pecu. 22, 143–55.Google Scholar
Maria, A.N., Viveiros, A.T.M., Freitas, R.T.F. & Oliveira, A.V. (2006). Extenders and cryoprotectants for cooling and freezing of piracanjuba (Brycon orbignyanus) semen, an endangered Brazilian teleost fish. Aquaculture 260, 298–306.Google Scholar
Maria, A.N., Azevedo, H.C., Santos, J.P., Silva, C.A. & Carneiro, P.C.F. (2010). Semen characterization and sperm structure of the Amazon tambaqui Colossoma macropomum . J. Appl. Ichthyol. 26, 779–83.Google Scholar
Murgas, L.S.D, Franciscatto, R.T. & Santos, A.G.O. (2004). Avaliação espermática pós-descongelamento em piracanjuba (Brycon orbignyanus), Vallenciennes, 1849). Rev. Bras. Zootec. 32, 1810–4.Google Scholar
Mylonas, C.C., Fostier, A. & Zanuy, F. (2010). Broodstock management and hormonal manipulations of fish reproduction. General and Comparative Endocrinology 165, 516–34.Google Scholar
Nakayama, C.L. (2011). Reprodução induzida e criopreservação do sêmen de papa-terra Menticirrhus americanus (Perciformes: Sciaenidae). [Thesis]. Rio Grande do Sul, Universidade Federal do Rio Grande.Google Scholar
Ninhaus-Silveira, A., Foresti, F., Veríssimo-Silveira, R. & Senhorini, J. A. (2006). Seminal, analysis, cryogenic preservation, and fertility in matrinxã fish, Brycon cephalus (Günther, 1869). Brazil. Arch. Biol. Technol. 49, 651–9.Google Scholar
Oliveira, A.V., Viveiros, A.T.M., Maria, N.A., Freitas, R.T.F. & Izaú, Z.A. (2007). Sucesso do resfriamento e congelamento de sêmen de pirapitinga Brycon nattereri . Arq. Bras. Med. Vet. Zoo. 59, 1509–15.Google Scholar
Pardo-Carrasco, S.C., Zaniboni-Filho, E., Arias-Castellanos, J.Á., Suárez-Mahecha, H., Atencio-García, V.J. & Cruz-Casallas, P.E. (2006). Evaluation of milt quality of the yamú Brycon amazonicus under hormonal induction. Rev. Colom. Ciênc. Pecu. 19, 1–2.Google Scholar
Ramirez-Merlano, J., Medina-Robles, V.M. & Cruz-Casallas, P.E. (2011). Variación estacional de las características seminales del bagre rayado Pseudoplatystoma metaense (Telostei, Pimelodidae). Rev. MVZ Córdoba 16, 2336–48.CrossRefGoogle Scholar
Reis, R.E., Kullander, S.O. & Ferraris Junior, C.J. (2003). Checklist of the Freshwater Fishes of South and Central America. Porto Alegre: EDIPUCRS, 729 pp.Google Scholar
Romagosa, E., Narahara, M.Y., Borella, M.I. & Fenerich-Verani, N. (2001). Seleção e caracterização de fêmeas de matrinxã, Brycon cephalus, induzidas a reprodução. Bol. Inst. Pesca 27, 139–47.Google Scholar
Sanches, E.A., Bombardelli, R.A., Baggio, D.M. & Souza, B.E. (2009). Dose inseminante para fertilização artificial de ovócitos de dourado. Rev. Bras. Zoo. 38, 2091–8.Google Scholar
Sanches, E.A., Marcos, R.M., Baggio, D.M., Tessaro, L., Balen, R.E. & Bombardelli, R.A. (2011). Estimativa da concentração espermática do sêmen de peixe pelo método de espermatócrito. Rev. Bras. Zoo 40, 1163–7.Google Scholar
Sato, Y., Sampaio, E.V., Ferenich-Verani, N. & Verani, J.R. (2006). Biologia reprodutiva e reprodução induzida de duas espécies de Characidae (Osteichthyes, Characiformes) da Bacia do São Francisco, Minas Gerais, Brasil. Rev. Bras. Zoo. 23, 267–73.CrossRefGoogle Scholar
Shimoda, E., Andrade, D.R., Vidal Júnior, M.V., Yasui, G.S., Silva, J.F.S., Godinho, H. P. & Souza, G. (2007). Efeitos da osmolaridade sobre a motilidade espermática na piabanha Brycon insignis . Rev. Ceres 54, 430–3.Google Scholar
Streit Junior, D.P., Ribeiro, R.P., Moraes, G.V., Mendez, L.V., Gallo, J.M., Digmayer, M. & Povh, J.A. (2006). Características qualitativas do sêmen de pacu (Piaractus mesopotamicus) após indução hormonal. Biosci. J. 22, 119–25.Google Scholar
Streit Junior, D. P., Sirol, R.N., Ribeiro, R.P., Moraes, G.V., Vargas, L.D.M., Watanabe, A.L. (2008). Qualitative parameters of the piapara semen (Leporinus elongatus Valenciennes, 1850). Brazilian J. Biology 68, 373–7.CrossRefGoogle Scholar
Streit Junior, D.P., Sirol, R.N., Ribeiro, R.P., Digmayer, M., Gallo, J.M., Moraes, G.V. & Povh, J.A. (2012). Parâmetros seminais de reprodutores de Pseudoplatystoma reticulatum, em cativeiro, pré e pós-indução hormonal. Rev. Bras. Reprod. Anim. 36, 188–93.Google Scholar
Targońska, K., Kucharczyk, D., Żarski, D., Cejko, B.I., Krejszeff, S., Kupren, K., Król, R., Dryl, K., Kowalski, R. K. & Glogowski, J. (2011). Artificial reproduction of wild and cultured barbel Barbus barbus (Cyprinidae) under controlled conditions. Acta Vet. Hungarica 59, 363–72.Google Scholar
Viveiros, A.T.M. & Godinho, H.P. (2009). Sperm quality and cryopreservation of Brazilian freshwater fish species: a review. Fish Physiol. Biochem. 35, 137–50.Google Scholar
Viveiros, A.T.M., Amaral, T.B., Orfão, L.H., Isaú, Z.A., Caneppele, D. & Leal, M.C. (2011). Sperm cryopreservation of tiete tetra Brycon insignis (Characiformes): effects of cryoprotectants, extenders, thawing temperatures and activating agents on motility features. Aquac. Res. 42, 858–65.CrossRefGoogle Scholar
Viveiros, A.T.M., Maria, A.N., Orfão, L.H., Nascimento, A.F., Corrêa, F.M., Caneppele, D. (2012). Effects of extenders, cryoprotectants and freezing methods on sperm quality of the threatened Brazilian freshwater fish pirapitinga-do-sul Brycon opalinus (Characiformes). Theriogenology 1–8.Google Scholar
Zarski, D., Kucharczyk, D., Targońska, K., Jamróz, M., Krejszeff, S. & Mamcarz, A. (2009). Application of Ovopel and ovaprim and their combinations in controlled reproduction of two rheophilic cyprinid fish species. Polish J. Nat. Sci. 24, 235–44.Google Scholar
Woynarovich, E. & Horváth, L.A. (1983). Propagação artificial de peixes de águas tropicais. Brasília: FAO/CODEVASF–CNPq, 220 pp.Google Scholar