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Hormonal induction of Brycon cephalus (Characiformes, Characidae) to spermiation using D-ala6, pro9net-mGnRH + metoclopramide

Published online by Cambridge University Press:  15 July 2015

Cristiane Bashiyo-Silva ,
Raphael da Silva Costa ,
Douglas de Castro Ribeiro ,
José Augusto Senhorini ,
Rosicleire Veríssimo-Silveira  and
Alexandre Ninhaus-Silveira
Cristiane Bashiyo-Silva
Affiliation:
Laboratório de Ictiologia Neotropical - LINEO, Depto de Biologia e Zootecnia, FEIS/UNESP – Universidade Estadual Paulista ‘Júlio de Mesquita Filho’, Rua Monção, 226 – Zona Norte 15385–000 – Ilha Solteira, São Paulo, Brazil.
Raphael da Silva Costa
Affiliation:
Laboratório de Ictiologia Neotropical - LINEO, Depto de Biologia e Zootecnia, FEIS/UNESP – Universidade Estadual Paulista ‘Júlio de Mesquita Filho’, Rua Monção, 226 – Zona Norte 15385–000 – Ilha Solteira, São Paulo, Brazil.
Douglas de Castro Ribeiro
Affiliation:
Laboratório de Ictiologia Neotropical - LINEO, Depto de Biologia e Zootecnia, FEIS/UNESP – Universidade Estadual Paulista ‘Júlio de Mesquita Filho’, Rua Monção, 226 – Zona Norte 15385–000 – Ilha Solteira, São Paulo, Brazil.
José Augusto Senhorini
Affiliation:
Centro Nacional de Pesquisa e Conservação de Peixes Continentais (CEPTA/ICMBio), Rodovia SP-201 (Pref. Euberto Nemésio Pereira de Godoy), Km 6.5, 13630–970 – Pirassununga, São Paulo, Brazil.
Rosicleire Veríssimo-Silveira
Affiliation:
Laboratório de Ictiologia Neotropical - LINEO, Depto de Biologia e Zootecnia, FEIS/UNESP – Universidade Estadual Paulista ‘Júlio de Mesquita Filho’, Rua Monção, 226 – Zona Norte 15385–000 – Ilha Solteira, São Paulo, Brazil.
Alexandre Ninhaus-Silveira*
Affiliation:
Department of Biology and Zootechny, FEIS/UNESP – State University of São Paulo ‘Júlio de Mesquita Filho’, Rua Monção, 226, Zona Norte, Ilha Solteira, SP, 15385–000, Brazil.
*
All correspondence to: Alexandre Ninhaus-Silveira. Department of Biology and Zootechny, FEIS/UNESP – State University of São Paulo ‘Júlio de Mesquita Filho’, Rua Monção, 226, Zona Norte, Ilha Solteira, SP, 15385–000, Brazil. Tel: +55 18-37431255. Fax: +55 18-37422140. E-mail: ninhaus@bio.feis.unesp.br
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Summary

This study aimed to establish a hormonal induction protocol for spermiation of Brycon cephalus males, using Ala6, Pro9Net-mGnRH + metoclopramide (Ovopel®). Thus, 20 males were used divided into three inductor treatments [⅓ pellet/kg (T1), ⅔ pellet/kg (T2) and 1⅓ pellet/kg (T3)] and one control group (CO), which only received physiological solution applications (0.9% NaCl). All treatments were applied in a single dose. For evaluation of the availability of the treatment, the following seminal parameters were analyzed: seminal volume, subjective spermatic motility, duration of motility, pH, osmolality and spermatic concentration. T3 showed the highest seminal volume (4.66 ± 1.52 ml), and was significantly different in comparison with T1 (2.0 ± 0.9 ml), T2 (3.5 ± 1.3 ml) and CO (2.3 ± 1.2 ml). In relation to spermatic motility, T2 and T3 showed significantly higher levels [5, (81–100%)]. However, T3 showed significantly lower average sperm motility duration than T1, T2 and CO (30 ± 7 s; 28 ± 6 s; 32 ± 8 s, respectively). With regard to the seminal parameters of spermatic concentration, pH and osmolality, no significant variation was verified among treatments. In conclusion, mGnRH + metoclopramide used for hormonal induction of B. cephalus reproduction does not induce changes related to spermatic concentration, pH and osmolality parameters of the seminal fluid and the most adequate doses among tested treatments were ⅔ pellet/kg live fish.

Keywords

Induced spawningNeotropical fishSemen qualitySeminal characteristics
Type
Research Article
Information
Zygote , Volume 24 , Issue 3 , June 2016 , pp. 319 - 325
Copyright
Copyright © Cambridge University Press 2015 

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