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Impact of in vitro fertilization of bovine oocytes with sex-sorted frozen–thawed spermatozoa on developmental kinetics, quality and sex ratio of developing embryos

Published online by Cambridge University Press:  29 January 2010

J. Peippo ,
M. Räty ,
K. Korhonen ,
M. Eronen ,
K. Kananen ,
T. Hurme ,
M. Halmekytö  and
A. Mäki-Tanila
J. Peippo*
Affiliation:
MTT Agrifood Research Finland, Biotechnology and Food Research, FI-31600 Jokioinen, Finland.
M. Räty
Affiliation:
MTT Agrifood Research Finland, Biotechnology and Food Research, FI-31600 Jokioinen, Finland.
K. Korhonen
Affiliation:
MTT Agrifood Research Finland, Biotechnology and Food Research, FI-31600 Jokioinen, Finland.
M. Eronen
Affiliation:
University of Kuopio, Department of Biosciences, FI-70211 Kuopio, Finland.
K. Kananen
Affiliation:
University of Kuopio, Department of Biosciences, FI-70211 Kuopio, Finland.
T. Hurme
Affiliation:
MTT Agrifood Research Finland, Services Unit, FI-31600 Jokioinen, Finland.
M. Halmekytö
Affiliation:
University of Kuopio, Department of Biosciences, FI-70211 Kuopio, Finland.
A. Mäki-Tanila
Affiliation:
MTT Agrifood Research Finland, Biotechnology and Food Research, FI-31600 Jokioinen, Finland.
*
All correspondence to: J. Peippo. MTT Agrifood Research Finland, Biotechnology and Food Research, FI-31600 Jokioinen, Finland. e-mail: jaana.peippo@mtt.fi
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Summary

We studied whether bovine embryos developing after in vitro fertilization (IVF) with sex-sorted spermatozoa differed in developmental kinetics, quality and sex ratio from embryos produced with unsorted spermatozoa. Abattoir-derived oocytes were fertilized with X-sorted, Y-sorted or unsorted spermatozoa from a single bull. To evaluate economical use of the sex-sorted spermatozoa, washed spermatozoa from a single straw (2 million spermatozoa) were used to fertilize each batch of collected oocytes without any further isolation steps. Concentration of the unsorted spermatozoa was adjusted accordingly. Fertilizations were assessed by staining sperm asters at 10 hpi and pronuclei at 20 hpi. Embryo development and morphological quality were monitored on days 2, 7, 8 and 9 of the development (IVF = day 0). All embryos were sexed using PCR. Following fertilization, penetration and subsequent cleavage rates were compromised in the X-sorted group compared with the Y-sorted and unsorted groups (penetration: 58.0% vs. 89.8% and 90.0%, cleavage: 65.3% vs. 81.5% and 75.0%). The use of the sex-sorted spermatozoa did not, however, reduce the proportion of transferable embryos (sex-sorted 29.6% vs. unsorted 27.7%) or their quality (quality 1: sex-sorted 36.0% vs. unsorted 19.9%). The Y-sorted spermatozoa produced more transferable embryos of better quality than the X-sorted spermatozoa (days 7–8: 31.9% vs. 26.4%, quality 1: 38.9% vs. 30.6%). On average, out of 10 transferable embryos, nine were of the predicted sex in the X- and Y-sorted spermatozoa groups. These results indicate that low numbers of X- and Y-sorted spermatozoa can be used successfully for female and male embryo production in vitro.

Keywords

CattleEmbryo sexingFlow cytometrySexual dimorphismSperm aster
Type
Research Article
Information
Zygote , Volume 18 , Issue 3 , August 2010 , pp. 185 - 194
Copyright
Copyright © Cambridge University Press 2010

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