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Individual differences in the distribution of sperm acrosome-associated 1 proteins among male patients of infertile couples; their possible impact on outcomes of conventional in vitro fertilization

Published online by Cambridge University Press:  17 May 2016

K. Kishida ,
H. Harayama ,
F. Kimura  and
T. Murakami
K. Kishida*
Affiliation:
Laboratory of Reproductive Biology, Division of Animal Science, Department of Bioresource Science, Graduate School of Agricultural Science, Kobe University, 1 Rokko-dai, Nada, Kobe 657–8501, Japan. Department of Obstetrics and Gynecology, Shiga University of Medical Science, Otsu 520–2192, Japan.
H. Harayama*
Affiliation:
Laboratory of Reproductive Biology, Division of Animal Science, Department of Bioresource Science, Graduate School of Agricultural Science, Kobe University, 1 Rokko-dai, Nada, Kobe 657–8501, Japan.
F. Kimura
Affiliation:
Department of Obstetrics and Gynecology, Shiga University of Medical Science, Otsu 520–2192, Japan.
T. Murakami
Affiliation:
Department of Obstetrics and Gynecology, Shiga University of Medical Science, Otsu 520–2192, Japan.
*
All correspondence to: Kazumi Kishida or Hiroshi Harayama. Laboratory of Reproductive Biology, Division of Animal Science, Department of Bioresource Science, Graduate School of Agricultural Science, Kobe University, 1 Rokko-dai, Nada, Kobe 657–8501, Japan. Tel: +81 78 803 6552. Fax: +81 78 803 5807. E-mail: azuki@belle.shiga-med.ac.jp (KK) or harayama@kobe-u.ac.jp (HH)
All correspondence to: Kazumi Kishida or Hiroshi Harayama. Laboratory of Reproductive Biology, Division of Animal Science, Department of Bioresource Science, Graduate School of Agricultural Science, Kobe University, 1 Rokko-dai, Nada, Kobe 657–8501, Japan. Tel: +81 78 803 6552. Fax: +81 78 803 5807. E-mail: azuki@belle.shiga-med.ac.jp (KK) or harayama@kobe-u.ac.jp (HH)
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Summary

The aims of this study were to show the existence of individual differences in the distribution of sperm acrosome-associated 1 (SPACA1) among male patients of infertile couples and to examine their possible impact on the outcomes of conventional in vitro fertilization (IVF). The spermatozoa were collected from male patients of infertile couples, washed by centrifugation, collected by the swim-up method, and then used for clinical treatments of conventional IVF. The surplus sperm samples were fixed and stained with an anti-SPACA1 polyclonal antibody for the immunocytochemistry. In the clinical IVF treatments, fertilization rates and blastocyst development rates were evaluated. The immunocytochemical observations revealed that SPACA1 were localized definitely in the acrosomal equatorial segment and variedly in the acrosomal principal segment. Specifically, the detection patterns of SPACA1 in the acrosomal principal segment could be classified into three categories: (A) strong, (B) intermediate or faint, and (C) almost no immunofluorescence. The SPACA1 indexes were largely different among male patients with the wide range from 13 to 199 points. The SPACA1 indexes were significantly correlated with developmental rates of embryos to blastocysts (r = 0.829, P = 0.00162), although they were barely associated with fertilization rates at 19 h after insemination (r = 0.289, P = 0.389). These results suggest that the distribution of SPACA1 in sperm affects the outcomes of conventional IVF. In conclusion, this study provides initial data to promote large-scale clinical investigation to demonstrate that the SPACA1 indexes are valid as molecular biomarkers that can predict the effectiveness of conventional IVF of infertile couples.

Keywords

AcrosomeBiomarkerBlastocystInfertilityIVFSAMP32SPACA1
Type
Research Article
Information
Zygote , Volume 24 , Issue 5 , October 2016 , pp. 654 - 661
Copyright
Copyright © Cambridge University Press 2016 

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