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Interaction between oocytes, cortical germ cells and granulosa cells of the mouse and bat, following the dissociation–re-aggregation of adult ovaries

Published online by Cambridge University Press:  03 March 2020

Tania Janeth Porras-Gómez  and
Norma Moreno-Mendoza Open the ORCID record for Norma Moreno-Mendoza [Opens in a new window]
Tania Janeth Porras-Gómez
Affiliation:
Laboratorio de Biología Tisular y Reproductora, Departamento de Biología Comparada, Facultad de Ciencias, Universidad Nacional Autónoma de México
Norma Moreno-Mendoza*
Affiliation:
Department of Cell Biology and Physiology, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad Universitaria, 04510México, DF, México
*
Author for correspondence: Norma Moreno-Mendoza. Department of Cell Biology and Physiology, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad Universitaria, Apartado Postal 70228 México, D.F.04510México. Tel: +52 55 56 22 38 66. Fax: +52 55 55 50 38 93. E-mail: angelica@biomedicas.unam.mx
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Summary

It is widely accepted that the oocyte plays a very active role in promoting the growth of the follicle by directing the differentiation of granulosa cells and secreting paracrine growth factors. In turn, granulosa cells regulate the development of the oocytes, establishing close bidirectional communication between germ and somatic cells. The presence of cortical cells with morphological characteristics, similar to primordial germ cells that express specific germline markers, stem cells and cell proliferation, known as adult cortical germ cells (ACGC) have been reported in phyllostomid bats. Using magnetic cell separation techniques, dissociation–cellular re-aggregation and organ culture, the behaviour of oocytes and ACGC was analyzed by interacting in vitro with mouse ovarian cells. Bat ACGC was mixed with disaggregated ovaries from a transgenic mouse that expressed green fluorescent protein. The in vitro reconstruction of the re-aggregates was evaluated. We examined the viability, integration, cellular interaction and ovarian morphogenesis by detecting the expression of Vasa, pH3, Cx43 and Laminin. Our results showed that the interaction between ovarian cells is carried out in the adult ovary of two species, without them losing their capacity to form follicular structures, even after having been enzymatically dissociated.

Keywords

Adult cortical germ cellsChimeric ovaryGranulosa cellsOvary batOvary mouse
Type
Research Article
Information
Zygote , Volume 28 , Issue 3 , June 2020 , pp. 223 - 232
Copyright
© Cambridge University Press 2020

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