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Intermediate filament proteins immunologically related to cytokeratins in the oocyte of the fish Cyprinus carpio

Published online by Cambridge University Press:  26 September 2008

Caterina Mencarelli  and
Franco Cotelli
Caterina Mencarelli*
Affiliation:
Department of Evolutionary Biology, University of Siena and Department of Biology, University of Milan, Italy
Franco Cotelli
Affiliation:
Department of Evolutionary Biology, University of Siena and Department of Biology, University of Milan, Italy
*
Caterina Mencarelli, Department of Evolutionary Biology, University of Siena, Via Mattioli 4, 53100 Siena, Italy. Tel: +39 577 298894. Fax: +39 577 298898. e-mail: mencarelli@unisi.it.
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Summary

We have used monoclonal antibodies specific for different sets of human cytokeratins and the anti-IFA (Intermediate Filament Antigen) antibody to investigate the expression of intermediate filament proteins in the mature oocyte of the teleostCyprinus carpio. Several polypeptides have been identified, showing molecular weights ranging from 43 to 65kDa. Two-dimensional analysis of the immunoreactive species revealed the presence of at least six major protein spots and a series of minor components, grouped in quite a narrow pI range from 5.52 to 6.28. The general complexity of the carp oocyte cytokeratin-related cytoskeleton appears to be higher than those described for oocytes of other vertebrate species.

Keywords

CytokeratinsIntermediate filamentsOocyte cytoskeleton
Type
Article
Information
Zygote , Volume 5 , Issue 3 , August 1997 , pp. 207 - 212
Copyright
Copyright © Cambridge University Press 1997

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