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Isolation of viable sperm cells from tobacco (Nicotiana tabacum)

Published online by Cambridge University Press:  26 September 2008

Yajuan Cao ,
Adonis Reece  and
Scott D. Russell
Yajuan Cao
Affiliation:
Department of Botany and Microbiology, University of Oklahoma, Norman, Oklahoma, USA.
Adonis Reece
Affiliation:
Department of Botany and Microbiology, University of Oklahoma, Norman, Oklahoma, USA.
Scott D. Russell*
Affiliation:
Department of Botany and Microbiology, University of Oklahoma, Norman, Oklahoma, USA.
*
Scott D. Russell, Deparment of Botany and Microbiology, University of Oklahoma, Norman, OK 73019-0245, USA. Telephone: +1-405-325-6234. Fax: +1-405-325-7619. e-mail: srussell@ou.edu.
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Summary

Viable sperm cells of Nicotiana tabacum were isolated by the semi-vivo technique. After pollination, excised styles were floated, cut end immersed, in a solution of 15% sucrose with 0.01% boric and 0.03% Ca(NO3)2 at 27°C in a growth chamber until pollen tubes emerged. After sperm cells were formed (at least 8 h after pollination) tubes were immersed in a 9% mannitol solution. In this solution, sperm cells are nearly ellipsoidal and retain viability for over 6 h.

Keywords

Semi-vivoSperm isolationNicotiana tabacumTobacco
Type
Short communication
Information
Zygote , Volume 4 , Issue 2 , May 1996 , pp. 81 - 84
Copyright
Copyright © Cambridge University Press 1996

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