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lncRNA deleted in lymphocytic leukaemia 1 (DLEU1) promotes the migration and invasion of human embryonic trophoblast cells

Published online by Cambridge University Press:  09 September 2020

Zhongxiang Li Open the ORCID record for Zhongxiang Li [Opens in a new window]  and
Mingbin Hou
Zhongxiang Li*
Affiliation:
Department of Obstetrics, Weifang Traditional Chinese Hospital, Weifang, Shandong261000, China
Mingbin Hou
Affiliation:
Department of Anesthesiology, Weifang Maternal and Child Health Hospital, Weifang, Shandong261000, China
*
Author for correspondence: Zhongxiang Li. Department of Obstetrics, Weifang Traditional Chinese Hospital, No. 1055 Weizhou Road, Kuiwen District, Weifang, Shandong261000, China. E-mail: zhongxiangl2020@126.com
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Summary

To investigate the roles of lncRNA deleted in lymphocytic leukaemia 1 (DLEU1) on migration and invasion of human trophoblast cells. Human chorionic trophoblast cell line HTR8/SVneo was cultured and transfected using lncRNA DLEU1 small interfering RNA. Real-time quantitative polymerase chain reaction was used to detect lncRNA DLEU1 expression. The activity of migration regulatory protein CDC42 was detected by western blot. The downstream miRNA targets of lncRNA and mRNAs targeted by corresponding miRNAs were respectively predicted using bioinformatics analyses. Compared with the control group, the expression of lncRNA DLEU1 in the small interfering RNA group was significantly decreased (P < 0.05). There was no significant change in cell proliferation capacity for transfected cells (lncRNA DLEU1 siRNA-1, P = 0.537; lncRNA DLEU1 siRNA-2, P = 0.384), but cell migration (lncRNA DLEU1 siRNA-1, P = 0.025; lncRNA DLEU1 siRNA-2, P = 0.019) and invasion (lncRNA DLEU1 siRNA-1, P = 0.0327; lncRNA DLEU1 siRNA-2, P = 0.021) was significantly reduced. CDC42 activity in the lncRNA DLEU1 knockdown group decreased and the phosphorylation of cofilin increased. Therefore, downregulation of lncRNA DLEU1 suppressed the migration and invasion of human trophoblast cells.

Keywords

Cell invasionCell migrationlncRNA DLEU1ProliferationTrophoblast cells
Type
Research Article
Information
Zygote , Volume 28 , Issue 5 , October 2020 , pp. 397 - 402
Copyright
© The Author(s), 2020. Published by Cambridge University Press

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