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lncRNA FOXD2-AS1 affects trophoblast cell proliferation, invasion and migration through targeting miRNA

Published online by Cambridge University Press:  13 January 2020

Yulei Zhang  and
Xiaoqin Chen Open the ORCID record for Xiaoqin Chen [Opens in a new window]
Xiaoqin Chen*
Affiliation:
Obstetrics Department, Huai’an First People’s Hospital, Huaian, Jiangsu, 223300, China
*
Author for correspondence: Xiaoqin Chen. Huai’an First People’s Hospital, 1 Hunaghe West Road, Huaian, Jiangsu, 223300, China. E-mail: chenxiaoqin3c@126.com
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Summary

The abnormal expression of lncRNAs and miRNAs has been found in the placentas of patients with preeclampsia (PE). Therefore, we determined the role of lncRNA FOXD2-AS1/miR-3127 in trophoblast cells. The expression of lncRNA FOXD2-AS1 was detected by qRT-PCR. The proliferation, migration and invasion ability of trophoblast cells were evaluated using CCK-8, wound healing and transwell assays. The target gene of lncRNA FOXD2-AS1 was determined by StarBase and luciferase reporter assays. Western blotting was used to analyze the expression of matrix metalloproteinase 2 (MMP2) and matrix metalloproteinase 9 (MMP9). The results showed that FOXD2-AS1 affected trophoblast cell viability in vitro, while the expression of miR-3127 was decreased. FOXD2-AS1 silencing decreased the promotion effects on trophoblast cell induced by miR-3127 inhibition. In addition, FOXD2-AS1 and miR-3127 presented the same effect on MMP2 and MMP9 levels. lncRNA FOXD2-AS1 modulated trophoblast cell proliferation, invasion and migration through downregulating miR-3127 expression. Therefore, lncRNA FOXD2-AS1 could act as a latent therapeutic marker in preeclampsia.

Keywords

InvasionlncRNA FOXD2-AS1MigrationmiR-3127Proliferation
Type
Research Article
Information
Zygote , Volume 28 , Issue 2 , April 2020 , pp. 131 - 138
Copyright
© Cambridge University Press 2020

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