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Maternal versus paternal expression of a LacZ transgene in preimplantation mouse embryos: effects of genetic background and 2-cell block

Published online by Cambridge University Press:  06 August 2001

Irina Neganova ,
Martin Augustin ,
Ursula Eichenlaub-Ritter  and
Harald Jockusch
Irina Neganova
Affiliation:
Visiting guest from the Laboratory of Cell Morphology, Institute of Cytology, Russian Academy of Sciences, St Petersburg, Russia.
Martin Augustin
Affiliation:
Developmental Biology and Molecular Pathology, W7, University of Bielefeld, D-33501 Bielefeld, Germany. Present address: Ingenium Pharmaceutics AG, Department of Genomics, Fraunhoferstrasse 13, D-82152 Martinsried, Germany.
Ursula Eichenlaub-Ritter
Affiliation:
Gene Technology/Microbiology, W3, University of Bielefeld, D-33501 Bielefeld, Germany.
Harald Jockusch
Affiliation:
Developmental Biology and Molecular Pathology, W7, University of Bielefeld, D-33501 Bielefeld, Germany.
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Abstract

The expression of a transgene NI-ROSA LacZ (LacZtg) trapped into the genes for two presumably untranslated, ubiquitously expressed RNAs, was studied in preimplantation mouse embryos with respect to penetrance (fraction of expressing embryos) and to localisation of β-galactosidase activity. With maternal origin in NMRI mice β-galactosidase was first detected within one dot in the cytoplasm of zygotes at 30 h post-hCG. The staining pattern progressed to small clusters and to dense, homogeneous staining of the entire cytoplasm during further development. Within the NMRI background, penetrance in utero was delayed by at least 6 h when the transgene was of paternal as compared with maternal origin. Paternal transgene expression increased marginally during culture to 50 h after explantation of embryos at 30-48 h post-hCG and remained low or decreased in the ‘2-cell block’. Expression of a paternal transgene in preimplantation embryos developing in utero was further delayed in the maternal MF1 as compared with the NMRI background. In contrast to NMRI × NMRI embryos with paternally derived transgene, expression increased with time during the 2-cell block in MF1 × NMRI embryos. Thus, in the earliest phase of mammalian development expression of this LacZtg is influenced by parental origin, maternal genetic background and environment. The spatial distribution of the gene product is developmentally controlled.

Keywords

2-cell blockExpressionImprintingLacZ transgeneSpatial distribution
Type
Research Article
Information
Zygote , Volume 9 , Issue 3 , August 2001 , pp. 219 - 228
Copyright
© 2001 Cambridge University Press

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