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Mechanism of the adverse effect of hyaluronidase used for oocyte denudation on early development of bovine embryos

Published online by Cambridge University Press:  24 February 2021

Shiori Ashibe Open the ORCID record for Shiori Ashibe [Opens in a new window] ,
Kanade Irisawa ,
Ken Yokawa  and
Yoshikazu Nagao
Shiori Ashibe
Affiliation:
Department of Animal Production Science, United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Tokyo, Japan University Farm, Faculty of Agriculture, Utsunomiya University, Tochigi, Japan
Kanade Irisawa
Affiliation:
University Farm, Faculty of Agriculture, Utsunomiya University, Tochigi, Japan
Ken Yokawa
Affiliation:
Center for Bioscience Research and Education, Utsunomiya University, Tochigi, Japan
Yoshikazu Nagao*
Affiliation:
Department of Animal Production Science, United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Tokyo, Japan University Farm, Faculty of Agriculture, Utsunomiya University, Tochigi, Japan
*
Author for correspondence: Yoshikazu Nagao. University Farm, Faculty of Agriculture, Utsunomiya University, Tochigi, Japan. Tel: +81 285 84 1321. Fax: +81 285 84 1321. E-mail: ynagao@cc.utsunomiya-u.ac.jp
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Summary

Hyaluronidase is widely used in animal and human assisted reproductive technologies (ARTs) to remove cumulus cells around oocytes. However, adverse effects of hyaluronidase treatment, such as increased rates of degeneration and parthenogenesis, have been found after treatment of human and mouse oocytes. Currently, the mechanism(s) of the detrimental effects are unclear. The present study was initiated to identify the mechanism of adverse responses to hyaluronidase treatment in bovine oocytes and early embryos. Cumulus cells were removed from cumulus–oocyte complexes (COCs) with or without hyaluronidase and the oocytes were subjected to intracytoplasmic sperm injection (ICSI) or in vitro fertilization (IVF). Significantly lower rates of blastocyst formation were obtained in the hyaluronidase treatment group after ICSI (22.4%) and IVF (21.2%) compared with the non-hyaluronidase control groups: 36.1% after ICSI and 30.4% after IVF. Next, we examined the effect of hyaluronidase on parthenogenetic development rates and on the cytoplasmic levels of free calcium ions (Ca2+), reactive oxygen species (ROS) and reduced glutathione (GSH). No differences in parthenogenesis rates were found between treated and untreated groups. Ca2+ levels in oocytes from the hyaluronidase treatment group indicated using mean fluorescence intensity were significantly higher (68.8 ± 5.3) compared with in the control group (45.0 ± 2.5). No differences were found in the levels of ROS or GSH between the treated and untreated groups. We conclude that hyaluronidase might trigger an increase in Ca2+ levels in oocytes, resulting in a decreased potential for normal embryonic development.

Keywords

Bovine oocytesFree calcium ions (Ca2+)HyaluronidaseIn vitro fertilization (IVF)Intracytoplasmic sperm injection (ICSI)
Type
Research Article
Information
Zygote , Volume 29 , Issue 5 , October 2021 , pp. 337 - 341
Copyright
© The Author(s), 2021. Published by Cambridge University Press

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Footnotes

*

Present address: School of Earth, Energy and Environmental Engineering, Faculty of Engineering, Kitami, Institute of Technology, Hokkaido, Japan

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