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Microtubule assembly and in vitro development of bovine oocytes with increased intracellular glutathione level prior to vitrification and in vitro fertilization

Published online by Cambridge University Press:  26 April 2013

H. Hara ,
I. Yamane ,
I. Noto ,
N. Kagawa ,
M. Kuwayama ,
M. Hirabayashi  and
S. Hochi
H. Hara
Affiliation:
Interdisciplinary Graduate School of Science and Technology, Shinshu University, Ueda, Nagano 386–8567, Japan.
I. Yamane
Affiliation:
Graduate School of Science and Technology, Shinshu University, Ueda, Nagano 386–8567, Japan.
I. Noto
Affiliation:
Graduate School of Science and Technology, Shinshu University, Ueda, Nagano 386–8567, Japan.
N. Kagawa
Affiliation:
Kato Ladies Clinic, Shinjuku, Tokyo 160–0023, Japan.
M. Kuwayama
Affiliation:
Repro-Support Medical Research Centre, Shinjuku, Tokyo 160–0022, Japan.
M. Hirabayashi
Affiliation:
National Institute for Physiological Sciences, Okazaki, Aichi 444–8787, Japan. The Graduate University for Advanced Studies, Okazaki, Aichi 444–8787, Japan.
S. Hochi*
Affiliation:
Interdisciplinary Graduate School of Science and Technology, Shinshu University, Ueda, Nagano 386–8567, Japan. Graduate School of Science and Technology, Shinshu University, Ueda, Nagano 386–8567, Japan. Faculty of Textile Science and Technology, Shinshu University, Ueda, Nagano 386–8567, Japan.
*
All correspondence to: S. Hochi. Interdisciplinary Graduate School of Science and Technology, Shinshu University, Ueda, Nagano 386–8567, Japan. Tel: +81 268 215350. Fax: +81 268 215830. e-mail: shochi@shinshu-u.ac.jp
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Summary

Although vitrification is a useful technique for preservation of bovine oocytes, the yield of blastocysts derived from the vitrified oocytes is still low. We have recently reported a new type of cryoinjury, multiple aster formation, by which pronuclear migration and development of vitrified–warmed and in vitro-fertilized bovine oocytes are impaired. The aim of the present study was to investigate the effect of glutathione (GSH) content of vitrified bovine oocytes on multiple aster formation and subsequent in vitro development. Treatment of bovine cumulus–oocyte complexes with β-mercaptoethanol (βME) and l-cysteine (Cys) during in vitro maturation resulted in 2.5-fold higher GSH content not only in fresh control but also in vitrified–warmed oocytes. The percentage of normally fertilized zygotes exhibiting sperm aster(s) was >95% in all four groups (with or without βME/Cys × fresh control or vitrified). The frequency of multiple aster formation in vitrified oocytes (three-fold higher than that in fresh control oocytes) was not affected by the increased level of intracellular GSH with βME/Cys. Consequently, the migration and development of pronuclei as well as the yield of blastocysts from vitrified–warmed oocytes (17 versus 41%) were not improved. In addition, there was no effect of increased GSH level on the yield of blastocysts in fresh control groups.

Keywords

Bovine oocytesIntracellular glutathioneβ-MercaptoethanolMultiple astersVitrification
Type
Research Article
Information
Zygote , Volume 22 , Issue 4 , November 2014 , pp. 476 - 482
Copyright
Copyright © Cambridge University Press 2013 

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