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mInscuteable regulates meiotic spindle organization during mouse oocyte meiotic maturation

Published online by Cambridge University Press:  18 November 2019

Zhuoni Xiao ,
Jiali Peng Open the ORCID record for Jiali Peng [Opens in a new window] ,
Meiting Xie ,
Jing Yang  and
Wangming Xu
Zhuoni Xiao*
Affiliation:
Reproductive Medical Center, Wuhan University Renmin Hospital, Wuhan, China
Jiali Peng
Affiliation:
Reproductive Medical Center, Wuhan University Renmin Hospital, Wuhan, China
Meiting Xie
Affiliation:
Reproductive Medical Center, Wuhan University Renmin Hospital, Wuhan, China
Jing Yang
Affiliation:
Reproductive Medical Center, Wuhan University Renmin Hospital, Wuhan, China
Wangming Xu
Affiliation:
Reproductive Medical Center, Wuhan University Renmin Hospital, Wuhan, China
*
Author for correspondence: Zhuoni Xiao. Reproductive Medical Center, Wuhan University, Renmin Hospital, 238 Jiefang Road, Wuhan, China Tel: +86 18627793566. E-mail: zhuonixiao@gmail.com
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Summary

Establishment of cellular polarity is one of the key events during oocyte maturation. Inscuteable (Insc) has been identified as a key regulator of cell polarity during asymmetric division in Drosophila. However, the function of its evolutionarily conserved mammalian homologue, mInscuteable (mInsc), in mouse meiotic maturation is not clear. In this study, we investigated the roles of mInsc in mouse oocyte maturation. mInsc was detected at all stages of oocyte maturation. The protein level of mInsc was slightly higher at the germinal vesicle breakdown (GVBD) stage and remained constant during mouse oocyte maturation. The subcellular localization of mInsc overlapped with spindle microtubules. Disruption of microtubules and microfilaments caused changes in the localization of mInsc. Depletion or overexpression of mInsc significantly decreased the maturation rates of mouse oocytes. Depletion of mInsc significantly affected asymmetric division, spindle assembly, alignments of chromosomes and actin cap formation. Taken together, our results demonstrated that mInsc regulates meiotic spindle organization during mouse meiotic maturation.

Keywords

Cell polarityInscuteableMeiosismInscOocyte maturationSpinal organization
Type
Research Article
Information
Zygote , Volume 28 , Issue 1 , February 2020 , pp. 45 - 50
Copyright
© Cambridge University Press 2019 

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