Hostname: page-component-cd9895bd7-gvvz8 Total loading time: 0 Render date: 2024-12-28T02:23:02.609Z Has data issue: false hasContentIssue false
  • English
  • Français

Modulators of Bufo arenarum ovulation

Published online by Cambridge University Press:  01 February 2008

Inés Ramos ,
Susana B. Cisint ,
Claudia A. Crespo ,
Marcela F. Medina  and
Silvia N. Fernández
Inés Ramos*
Affiliation:
Instituto Superior de Investigaciones Biológicas (INSIBIO), Departamento de Biología del Desarrollo, Universidad Nacional de Tucumán, Argentina, Chacabuco 461, 4.000 – Tucumán, Argentina.
Susana B. Cisint
Affiliation:
Instituto Superior de Investigaciones Biológicas (INSIBIO), Departamento de Biología del Desarrollo, Universidad Nacional de Tucumán, Argentina, Chacabuco 461, 4.000 – Tucumán, Argentina.
Claudia A. Crespo
Affiliation:
Instituto Superior de Investigaciones Biológicas (INSIBIO), Departamento de Biología del Desarrollo, Universidad Nacional de Tucumán, Argentina, Chacabuco 461, 4.000 – Tucumán, Argentina.
Marcela F. Medina
Affiliation:
Instituto Superior de Investigaciones Biológicas (INSIBIO), Departamento de Biología del Desarrollo, Universidad Nacional de Tucumán, Argentina, Chacabuco 461, 4.000 – Tucumán, Argentina.
Silvia N. Fernández
Affiliation:
Instituto Superior de Investigaciones Biológicas (INSIBIO), Departamento de Biología del Desarrollo, Universidad Nacional de Tucumán, Argentina, Chacabuco 461, 4.000 – Tucumán, Argentina.
*
1 All correspondence to: Inés Ramos. Instituto Superior de Investigaciones Biológicas (INSIBIO), Departamento de Biología del Desarrollo, Universidad Nacional de Tucumán, Argentina, Chacabuco 461, 4000 – S. M. de Tucumán, Argentina. Fax: +54 381 4247752 560. e-mail: inramos@fbqf.unt.edu.ar
Get access Rights & Permissions [Opens in a new window]

Summary

In this study we investigated ovulation in vitro using ovary samples from Bufo arenarum with respect to their response to stimulation with homologous pituitary homogenate (HPH) or with progesterone and prostaglandins (PGF2α and PGE1) as intermediates of pituitary action. Ovary samples were obtained from animals captured during the breeding period. Our results demonstrate that the ovulatory response to all different inducers was dose dependent, the highest percentage of ovulated oocytes being obtained with HPH treatment. An important increase in the ovulatory response was obtained by the association of PGF2α with either HPH or progesterone at suboptimal doses, indicating that this prostaglandin induced a synergistic potentiating effect. Incubation with cyclooxygenase inhibitors (indomethacin or diclofenac sodium) produced a significant decrease in the ovulation induced by HPH, demonstrating that prostaglandins are involved in the action of the pituitary gland in this process. According to our results, PGE1 not only had no participation in the ovulatory process, but also produced an inhibitory effect on ovulation induced by HPH treatment.

Keywords

AmphibianOvulationProgesteroneProstaglandin
Type
Research Article
Information
Zygote , Volume 16 , Issue 1 , February 2008 , pp. 65 - 72
Copyright
Copyright © Cambridge University Press 2008

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Chang, K.J., Kim, J.W., Im, W.B., Kang, H.M. & Kwon, H.B. (1997). Differential effects of gonadotropin and orthovanadate on oocyte maturation, ovulation and prostaglandin synthesis by Rana ovarian follicles in vitro. J. Exp. Zool. 277, 155–65.3.0.CO;2-O>CrossRefGoogle ScholarPubMed
de Atenor, M.B., de Romero, I.R., Brauckmann, E., Pisanó, A. & Legname, A.H. (1994). Effect of the pineal gland and melatonin on the metabolism of oocytes in vitro and on ovulation in Bufo arenarum. J. Exp. Zool. 268, 436–41.CrossRefGoogle Scholar
de Romero, I.R., de Atenor, M.B. & Legname, A.H. (1998). Nuclear maturation inhibitors in Bufo arenarum oocytes. Biocell 22, 27–34.Google ScholarPubMed
Espey, L.L. (1980). Ovulation as an inflammatory reaction – a hypothesis. Biol. Reprod. 22, 73–106.CrossRefGoogle ScholarPubMed
Espey, L.L., Tanaka, S., Stacy, S. & Okamura, H. (1992). Inhibition of ovulation in the gonadotropin-primed immature rat by exogenous prostaglandin E2. Prostaglandins 43, 67–74.CrossRefGoogle ScholarPubMed
Fernández, S.N. & Ramos, I. (2003). Endocrinology of Reproduction. In Reproductive Biology and Phylogeny of Anura, vol. 4 (ed. Jamieson, B.G.M.), pp. 73–117. Enfield, New Hampshire, USA: Science Publisher, Inc.Google Scholar
Fortune, J. E. (1983). Steroid production by Xenopus ovarian follicles at different developmental stages. Develop. Biol. 99, 502–9.CrossRefGoogle ScholarPubMed
Gobbetti, A. & Zerani, M. (1993). Possible roles for prostaglandins E2 and F2 alpha in seasonal changes in ovarian steroidogenesis in the frog (Rana esculenta). J. Reprod. Fertil. 98, 27–32.CrossRefGoogle ScholarPubMed
Goez, F.W., Duman, P., Ranjan, M. & Herman, C.A. (1989). Prostaglandin F and E synthesis by specific tissue components of the brook trout (Salvelinus fontinalis). J. Exp. Zool. 250, 196–205.Google Scholar
Hardeland, R., Pandi-Perumai, S.R. & Cardinali, D.P. (2006). Melatonin. Int. J. Biochem. Cell Biol. 38, 313–6.CrossRefGoogle ScholarPubMed
Hellberg, P., Larson, L., Olofsson, J., Brännström, M. & Hedin, L. (1996). Regulation of the inducible form of prostaglandin endoperoxide synthase in the perfused rat ovary. Mol. Hum. Reprod. 2, 111–6.CrossRefGoogle ScholarPubMed
Itoh, M. & Ishii, S. (1990). Changes in plasma levels of gonadotropins and sex steroids in the toad, Bufo japonicus, in association with behavior during the breeding season. Gen. Comp. Endocrinol. 80, 451–64.CrossRefGoogle ScholarPubMed
Jones, R.E., Orlicky, D.J., Austin, H.E., Rand, M.S. & Lopez, K.J. (1990). Indomethacin inhibits ovarian PGE secretion and gonadotropin-induced ovulation in a reptile (Anolis carolinensis). J. Exp. Zool. 255, 57–62.CrossRefGoogle Scholar
Kim, J.W., Im, W.B., Choi, H.H., Ishii, S. & Kwon, H.B. (1998). Seasonal fluctuations in pituitary gland and plasma levels of gonadotropic hormones in Rana. Gen. Comp. Endocrinol. 109, 13–23.CrossRefGoogle ScholarPubMed
Kwon, H.B., Chang, K.J., Yoo, Y.R., Lee, Ch. C. & Schuetz, A.W. (1992). Induction of ovulation and oocyte maturation of amphibian (Rana dybowski) ovarian follicles by protein kinase C activation in vitro. Biol. Reprod. 47, 169–76.CrossRefGoogle Scholar
Lessman, C.A. & Kessel, R.G. (1992). Effect of acrylamide on germinal vesicle migration and dissolution in oocytes of the frog, Rana pipiens. Exp. Cell Res. 202, 151–60.CrossRefGoogle ScholarPubMed
Maller, J.L. & Krebs, E.G. (1980). Regulation of oocyte maturation. Curr. Topics Cell Regul. 16, 271–311.CrossRefGoogle ScholarPubMed
Masui, Y. (1985). Meiotic arrest in animal oocytes. In Biology of Fertilization, vol. 1 (ed. , C.B. Metz & Monroy, A.), pp. 189–219. Orlando: Academic Press.CrossRefGoogle Scholar
Medina, M.F., Ramos, I., Crespo, C.A., González, S. & Fernández, S.N. (2004). Changes in serum sex steroid levels throughout the reproductive cycle of Bufo arenarum females. Gen. Comp. Endocrinol. 136, 143–51.CrossRefGoogle ScholarPubMed
Morril, G.A., Schatz, F., Kostellow, A. & Bloch, E. (2006). Gonadotropin stimulation of steroid síntesis and metabolism in the Rana pipiens ovarian follicle: sequential changes in endogenous steroids during ovulation, fertilization and cleavage stages. J. Steroid Biochem. Mol. Biol. 99, 129–38.CrossRefGoogle Scholar
Murdoch, W.J., Hansen, T.R. & McPherson, L.A. (1993). Role of eicosanoids in vertebrales ovulation. Prostaglandin 46, 85–115.CrossRefGoogle Scholar
Polzonetti-Magni, A.M., Mosconi, G., Carnevali, O., Yamamoto, K., Hanaoka, Y. & Kikuyama, S. (1998). Gonadotropins and reproductive function in the anuran amphibian Rana esculenta. Biol. Reprod. 58, 88–93.CrossRefGoogle ScholarPubMed
Ramos, I., Winik, B. C., Cisint, S., Crespo, C., Medina, M. & Fernández, S. N. (1999). Ultrastructural changes during nuclear maturation in Bufo arenarum oocytes. Zygote 7, 261–9.CrossRefGoogle ScholarPubMed
Ramos, I., Cisint, S., Medina, M.F., Crespo, C. & Fernández, S.N. (2005). Role of prolactin in nuclear maturation and ovulation in amphibian oocytes. Zygote 134, 265–68.CrossRefGoogle Scholar
Reich, R., Daphna-Iken, D., Chun, S.Y., Popliker, M., Slager, R., Adelmann-Grill, B.C., Tsafriri, A. (1991). Preovulatory changes in ovarian expression of collagenases and tissue metalloproteinase inhibitor messenger ribonucleic acid: role of eicosanoids. Endocrinology 129, 1869–75.CrossRefGoogle ScholarPubMed
Robker, R.L., Russell, D.L., Espey, L.L., Lydon, J.P., O'Malley, B.W. & Richards, J.S. (2000). Progesterone-regulated genes in the ovulation process: ADAMTS-1 and cathepsin L proteases. Proc. Natl. Acad. Sci. USA 97, 4689–94.CrossRefGoogle ScholarPubMed
Rose, U.M., Hanssen, E.G.J.M. & Kloosterboer, H.J. (1999). Development and characterization of an in vitro ovulation model using mouse ovarian follicles. Biol. Reprod. 61, 503–11.CrossRefGoogle Scholar
Schuetz, A.W. (1986). Hormonal dissociation of ovulation and maturation of oocytes. Ovulation of immature amphibian oocytes by prostaglandin. Gamete Res. 15, 99–113.CrossRefGoogle Scholar
Sirois, J. (1994). Induction of prostaglandin endoperoxide synthase-2 by human chorionic gonadotropin in bovine preovulatory follicles in vivo. Endocrinology 135, 841–8.CrossRefGoogle ScholarPubMed
Skoblina, M.N., Kondrat'eva, O.T., Nikiforova, G.P. & Huhtaniemi, I. (1997). The role of eicosanoids and progesterone in the ovulation of the oocytes in the common frog. Ontogenez 28, 211–6.Google ScholarPubMed
Sotowska-Brochocka, J., Martynska, L. & Licht, P. (1992). Changes of LH level in the pituitary gland and plasma in hibernating frogs, Rana temporaria. Gen. Comp. Endocrinol. 87, 286–91.CrossRefGoogle ScholarPubMed
Sotowska-Brochocka, J., Martynska, L. & Licht, P. (1994). Dopaminergic inhibition of gonadotropic release in hibernating frogs, Rana temporaria. Gen. Comp. Endocrinol. 93, 192–6.CrossRefGoogle ScholarPubMed
Takamura, K. & Kogo, H. (1989). The mode of action of indomethacin, aspirin and melatonin on the blockage of the first ovulation in immature rat pretreated with PMSG. Jpn J. Pharmacol. 50, 491–4.CrossRefGoogle Scholar
Valdez Toledo, C.L. & Pisanó, A. (1980). Fases ovogenéticas en Bufo arenarum. Reproduccion 4, 315–30.Google ScholarPubMed
Whittier, J. M. & Crews, D. (1987). Seasonal reproduction: patterns and control. In Hormone and Reproduction in Fishes, Amphibians and Reptiles, (ed. Norris, D.O. & Jones, R.E.), pp. 385–409. New York: Plenum Press.CrossRefGoogle Scholar