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Morphology and morphometry of preantral follicles, and immunolocalization of angiogenic factors in ovarian tissue from the neotropical primate Sapajus apella

Published online by Cambridge University Press:  19 November 2018

AB Brito ,
DCC Brito ,
W B Silva ,
APR Rodrigues ,
JR Figueiredo ,
SFS Domingues  and
RR Santos Open the ORCID record for RR Santos [Opens in a new window]
AB Brito
Affiliation:
Laboratory of Wild Animal Biology and Medicine, Faculty of Veterinary Medicine, Federal University of Pará, Belém, Pará, Brazil
DCC Brito
Affiliation:
Laboratory of Manipulation of Oocytes and Ovarian Preantral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, Ceará State University, Fortaleza, CE, Brazil
W B Silva
Affiliation:
National Primate Center, Ananindeua, Pará, Brazil
APR Rodrigues
Affiliation:
Laboratory of Manipulation of Oocytes and Ovarian Preantral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, Ceará State University, Fortaleza, CE, Brazil
JR Figueiredo
Affiliation:
Laboratory of Manipulation of Oocytes and Ovarian Preantral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, Ceará State University, Fortaleza, CE, Brazil
SFS Domingues
Affiliation:
Laboratory of Wild Animal Biology and Medicine, Faculty of Veterinary Medicine, Federal University of Pará, Belém, Pará, Brazil
RR Santos*
Affiliation:
Laboratory of Wild Animal Biology and Medicine, Faculty of Veterinary Medicine, Federal University of Pará, Belém, Pará, Brazil Schothorst Feed Research, The Netherlands
*
Address for correspondence: Regiane R Santos. Faculty of Veterinary Medicine, Federal University of Pará, Laboratory of Wild Animal Biology and Medicine, BR 316 Km 61, CEP 68740-970, Castanhal, Pará, Brazil. Tel: +55 91 33114707. E-mail: R.Rodriguesdossantos@pq.cnpq.br
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Summary

Ovarian biopsies from five health adult monkeys were collected by exploratory laparotomy. Preantral follicles (primordial, primary, and secondary) were classified as normal or degenerated and submitted to morphometric analysis in which granulosa cell counts and the areas of follicles, oocytes, and oocyte nuclei were measured. Ovarian fragments were also immunolabelled for the quantitative analysis of VEGFA and CD31 protein expression in the ovarian tissue and in the preantral follicles. In total, 213 preantral follicles was examined for morphometry and morphological classification. From this total, 20 (9.4%) were follicles enclosing two or more oocytes, i.e. multi-oocyte follicles (MOFs). From the 193 follicles enclosing only one oocyte, 46.3% were classified as primordial, 24,1% as transition, 23.3% as primary, and 6.3% as secondary follicles. The mean number of granulosa cells surrounding primordial, transition, primary, and secondary follicles was 9.2, 12.1, 18.7, and 45.3, respectively. Increase in oocyte diameter was observed from primary to secondary follicles, while the oocyte nucleus increased only when follicles reached the secondary stage. The expression of CD31 was strong in vessels, corpus luteum, and in normal oocytes and granulosa cells from preantral follicles at all developmental stages. Likewise, VEGFA expression was observed in vessels and preantral follicles (granulosa cells, the oocyte and the oocyte nucleus). We characterized the morphology, and morphometry and expression of angiogenic factors in normal and atretic preantral follicles from Sapajus apella. This description can support the analysis of follicular quality and survival after procedures such as transplantation and cryopreservation.

Keywords

Capuchin monkeysCD31Ovarian folliclesOvarian tissueVEGFA
Type
Research Article
Information
Zygote , Volume 26 , Issue 5 , October 2018 , pp. 424 - 429
Copyright
© Cambridge University Press 2018 

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