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Morphometric characterization of the first blastomeres of rainbow trout (Oncorhynchus mykiss)

Published online by Cambridge University Press:  18 January 2012

Iván I. Valdebenito ,
Rubén R. Sánchez ,
Brian R. Effer  and
Andrea M. Ubilla
Iván I. Valdebenito*
Affiliation:
Universidad Católica de Temuco, Facultad de Recursos Naturales, Escuela de Acuicultura, Rudecindo Ortega 02950 Casilla 15-D, Temuco, Chile.
Rubén R. Sánchez
Affiliation:
Universidad Católica de Temuco, Facultad de Recursos Naturales, Escuela de Acuicultura, Rudecindo Ortega 02950 Casilla 15-D, Temuco, Chile.
Brian R. Effer
Affiliation:
Universidad Católica de Temuco, Facultad de Recursos Naturales, Escuela de Acuicultura, Rudecindo Ortega 02950 Casilla 15-D, Temuco, Chile. Universidad del Magdalena, Facultad de Ingeniería, Programa de Ingeniería Pesquera, Carrera 32 No. 22-08 Sector San Pedro Alejandrino, Santa Marta, Colombia.
Andrea M. Ubilla
Affiliation:
Universidad Católica de Temuco, Facultad de Recursos Naturales, Escuela de Acuicultura, Rudecindo Ortega 02950 Casilla 15-D, Temuco, Chile.
*
All correspondence to: Iván I. Valdebenito. Universidad Católica de Temuco, Facultad de Recursos Naturales, Escuela de Acuicultura, Rudecindo Ortega 02950 Casilla 15-D, Temuco, Chile. Tel: +56 045 205510. e-mail: ivisler@uct.cl
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Summary

In the following investigation the morphometric characteristics of the first two blastomeres of rainbow trout (Oncorhynchus mykiss) were determined. Embryos were incubated at 9°C and then fixed in a Stockard solution every 30 min starting from 8.5 to 12.5 h of incubation post fertilization. Embryonic discs were extracted and microphotographs were taken with Q Capture Pro 5.0 software using a stereomicroscope Olympus SZX7. The average size of the blastodiscs was 941.22 ± 160.42 μm. The first cleavage finished after approximately 12 h of incubation. The first two blastomeres were regularly symmetrical in their morphology. Blastomere 1 had an average length (L) of 942.68 ± 105.56 μm and width (W) of 467.34 ± 64.33 μm. Blastomere 2 had an average length of 887.60 ± 101.65 and width of 454.49 ± 47.25 μm (n = 91). Significant differences were found between the length and width of blastomeres 1 and 2. The proportion between the length of blastomeres 1 and 2 was 0.94 ± 0.07 (n = 91); between the width of blastomeres 1 and 2 it was 0.88 ± 0.11 (n = 91); and the width/length ratio was 0.51 ± 0.09 (n = 182). It was concluded that rainbow trout blastomeres tend to be asymmetrical in length with a higher dispersion of widths.

Keywords

AsymmetricalEarly stageFirst blastomeresRainbow troutSymmetrical
Type
Research Article
Information
Zygote , Volume 20 , Issue 4 , November 2012 , pp. 327 - 331
Copyright
Copyright © Cambridge University Press 2012

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