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Na+/Ca2+ exchanger contributes to asterosap-induced elevation of intracellular Ca2+ concentration in starfish spermatozoa

Published online by Cambridge University Press:  01 May 2006

M. Sadiqul Islam ,
Osamu Kawase ,
Sumitaka Hase ,
Hiroyuki Minakata ,
Motonori Hoshi  and
Midori Matsumoto
M. Sadiqul Islam
Affiliation:
Department of Biosciences and Informatics, Keio University, 3-14-1, Hiyoshi, Kouhoku-ku, Yokohama 223-8522, Japan.
Osamu Kawase
Affiliation:
Department of Biosciences and Informatics, Keio University, 3-14-1, Hiyoshi, Kouhoku-ku, Yokohama 223-8522, Japan.
Sumitaka Hase
Affiliation:
Department of Biology, Keio University, 4-1-1, Hiyoshi, Kouhoku-ku, Yokohama 223-8521, Japan.
Hiroyuki Minakata
Affiliation:
SUNBOR, 1-1-1, Wakayamadai, Shimamotocho Mishimagun, Osaka 618-8503, Japan.
Motonori Hoshi
Affiliation:
Department of Biosciences and Informatics, Keio University, 3-14-1, Hiyoshi, Kouhoku-ku, Yokohama 223-8522, Japan.
Midori Matsumoto*
Affiliation:
Department of Biosciences and Informatics, Keio University, 3-14-1, Hiyoshi, Kouhoku-ku, Yokohama 223-8522, Japan.
*
All correspondence to: M. Matsumoto, Department of Biosciences and Informatics, Keio University, 3-14-1, Hiyoshi, Kouhoku-ku, Yokohama 223-8522, Japan. Tel: +81 045 5661774. Fax: +81 045 5661448. e-mail: mmatsumo@bio.keio.ac.jp
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Summary

Asterosap, a group of equally active isoforms of sperm-activating peptides from the egg jelly of the starfish Asterias amurensis, functions as a chemotactic factor for sperm. It transiently increases the intracellular cGMP level of sperm, which in turn induces a transient elevation of intracellular Ca2+ concentration ([Ca2+]i). Using a fluorescent Ca2+-sensitive dye, Fluo-4 AM, we measured the changes in sperm [Ca2+]i in response to asterosap. KB-R7943 (KB), a selective inhibitor of Na+/Ca2+ exchanger (NCX), significantly inhibited the asterosap-induced transient elevation of [Ca2+]i, suggesting that asterosap influences [Ca2+]i through activation of a K+-dependent NCX (NCKX). An NCKX activity of starfish sperm also shows K+ dependency like other NCKXs. Therefore, we cloned an NCKX from the starfish testes and predicted that it codes for a 616 amino acid protein that is a member of the NCKX family. Pharmacological evidence suggests that this exchanger participates in the asterosap-induced Ca2+ entry into sperm.

Keywords

AsterosapIntracellular Ca2+NCXNCKXStarfish
Type
Research Article
Information
Zygote , Volume 14 , Issue 2 , May 2006 , pp. 133 - 141
Copyright
Copyright © Cambridge University Press 2006

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