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Optimization of sperm freezability in Bactrian camel using various dilution rates and equilibration times

Published online by Cambridge University Press:  30 September 2019

Vahid Vahedi Open the ORCID record for Vahid Vahedi [Opens in a new window] ,
Mohsen Mostafaei ,
Hossein Vaseghi Dodaran  and
Nemat Hedayat Evrigh
Vahid Vahedi*
Affiliation:
Department of Animal Science, Moghan College of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran
Mohsen Mostafaei
Affiliation:
Research Centre for Agriculture and Natural Resources, Ardabil 56491-11169, Iran
Hossein Vaseghi Dodaran
Affiliation:
Department of Animal Science, College of Agriculture, University of Tabriz, Tabriz, Iran
Nemat Hedayat Evrigh*
Affiliation:
Department of Animal Science, Faculty of Agricultural Sciences, University of Mohaghegh Ardabili, Ardabil, Iran
*
Address for correspondence: Vahid Vahedi. Department of Animal Science, Moghan College of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran. Tel: +98 45 32715417. Mobile: +98 914 4322355. Fax: +98 45 32715417. E-mail: vahediv@uma.ac.ir Nemat Hedayat Evrigh. Department of Animal Science, Faculty of Agricultural Sciences, University of Mohaghegh Ardabili, Ardabil , Iran Fax: +98 45 33512204. E-mail: nhedayat@uma.ac.ir
Address for correspondence: Vahid Vahedi. Department of Animal Science, Moghan College of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran. Tel: +98 45 32715417. Mobile: +98 914 4322355. Fax: +98 45 32715417. E-mail: vahediv@uma.ac.ir Nemat Hedayat Evrigh. Department of Animal Science, Faculty of Agricultural Sciences, University of Mohaghegh Ardabili, Ardabil , Iran Fax: +98 45 33512204. E-mail: nhedayat@uma.ac.ir
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Summary

The effect of different dilution rates and equilibration times on the cryopreservation of Bactrian camel spermatozoa was evaluated in the current study. Semen samples from four healthy adult males were collected, processed and pooled. They were then subjected to a completely randomized 4×2 factorial design including four dilution rates (DR; 1:1, 1:2, 1:4 or 1:8; v:v with SHOTOR diluent) and two equilibration times (ET; 1 or 2 h at 5ºC). After freezing and thawing, sperm kinematic parameters as well as viability, plasma membrane integrity, abnormality and seminal malondialdehyde level were assessed. According to the results, four-fold diluted samples recorded significantly higher values (P < 0.05) for sperm total (39.58 vs 31.83 and 33.33,%) and progressive motility (19.50 vs 14.00 and 14.25,%), viability (55.37 vs 43.50 and 48.75,%) and plasma membrane integrity (46.75 vs 37.25 and 37.37,%) than those of both less (1:1) and high (1:8) concentrated samples, respectively. By contrast, the percentage of abnormal spermatozoa and the concentration of seminal malondialdehyde were comparable among all treated groups. Moreover, ET revealed that 1 h equilibration had significantly higher sperm motility (37.04 vs 33.33%), linearity (42.29 vs 32.26%), beat cross-frequency (13.15 vs 8.70 Hz), plasma membrane integrity (42.25 vs 39.75%) and viability (51.37 vs 48.12%) compared with 2 h of ET (P < 0.05). Taken together, a four-fold dilution along with 1 h equilibration can be an optimal procedure to cryopreserve Bactrian camel sperm.

Keywords

Bactrian camelDilution rateEquilibration timeSemen cryopreservation
Type
Research Article
Information
Zygote , Volume 27 , Issue 6 , December 2019 , pp. 362 - 366
Copyright
© Cambridge University Press 2019 

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