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Optimization of ultraviolet irradiation of Clarias gariepinus (Burchell, 1822) eggs for androgen production

Published online by Cambridge University Press:  15 January 2021

Victor Tosin Okomoda Open the ORCID record for Victor Tosin Okomoda [Opens in a new window] ,
Haziqah Jumahat Nursyaza ,
Ijabo Oga Samuel ,
Anuar Hassan ,
Abraham Sunday Oladimeji ,
Ambok Bolong Abol-Munafi ,
Korede Isaiah Alabi  and
Mhd Ikhwanuddin
Victor Tosin Okomoda*
Affiliation:
Department of Fisheries and Aquaculture, College of Forestry and Fisheries, University of Agriculture Makurdi, P.M.B. 2373Makurdi, Nigeria Higher Institution Centre of Excellence (HICoE), Institute of Tropical Aquaculture and Fisheries (AKUATROP), Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia
Haziqah Jumahat Nursyaza
Affiliation:
Higher Institution Centre of Excellence (HICoE), Institute of Tropical Aquaculture and Fisheries (AKUATROP), Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia
Ijabo Oga Samuel
Affiliation:
Department of Aquaculture, Faculty of Agriculture, Federal University of Gashua, P.M.B 1005Ilorin, Nigeria
Anuar Hassan
Affiliation:
Higher Institution Centre of Excellence (HICoE), Institute of Tropical Aquaculture and Fisheries (AKUATROP), Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia Faculty of Food Science and Fisheries, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia
Abraham Sunday Oladimeji
Affiliation:
Agricultural Department, National Biotechnology Development Agency (NABDA), Abuja, Nigeria
Ambok Bolong Abol-Munafi
Affiliation:
Department of Aquaculture, Faculty of Agriculture, Federal University of Gashua, P.M.B 1005Ilorin, Nigeria Faculty of Food Science and Fisheries, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia
Korede Isaiah Alabi
Affiliation:
Department of Agricultural Extension and Management, Federal College of Forestry, JosPlateau, Nigeria
Mhd Ikhwanuddin*
Affiliation:
Higher Institution Centre of Excellence (HICoE), Institute of Tropical Aquaculture and Fisheries (AKUATROP), Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia
*
Authors for correspondence: Victor Tosin Okomoda. Department of Fisheries and Aquaculture, College of Forestry and Fisheries, University of Agriculture Makurdi, P.M.B. 2373Makurdi, Nigeria. Tel: +23 48033319959. E-mail: okomodavictor@yahoo.com Ikhwanuddin Mhd. Institute of Tropical Aquaculture and Fisheries (AKUATROP), Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia. Tel: +60 199851868. E-mail: ikhwanuddin@umt.edu.my
Authors for correspondence: Victor Tosin Okomoda. Department of Fisheries and Aquaculture, College of Forestry and Fisheries, University of Agriculture Makurdi, P.M.B. 2373Makurdi, Nigeria. Tel: +23 48033319959. E-mail: okomodavictor@yahoo.com Ikhwanuddin Mhd. Institute of Tropical Aquaculture and Fisheries (AKUATROP), Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia. Tel: +60 199851868. E-mail: ikhwanuddin@umt.edu.my
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Summary

The optimum distance and duration of ultraviolet (UV) irradiation for the complete inactivation of African catfish Clarias gariepinus egg nucleus was investigated in this study. The UV light was suspended above the unfertilized eggs at four distances (5, 10, 20 and 30 cm) and for five durations (1, 2, 3, 4 and 5 min). Then, the irradiated eggs were activated with sperm from diploid C. gariepinus and cold shocked at 5°C for 5 min just moments before cell cleavage. Ploidy analysis was performed using karyotype chromosome counting. The results obtained suggested that the further the distance, the better the hatchability rate, however prolonged duration seemed to significantly reduced hatchability. All treatments with surviving progenies at the end of the study showed evidence of successfully diploid gynogen (2n = 56) induction at different percentages. However, the optimal protocol that gave a moderately high hatchability/survival rate and completely induced gynogens was exposure of the eggs to UV irradiation at 20 cm for 1 min. It was concluded that the distance and duration of UV irradiation affects gynogenetic induction in African catfish C. gariepinus.

Keywords

African catfishChromosome manipulationCold shockUV radiation
Type
Research Article
Information
Zygote , Volume 29 , Issue 3 , June 2021 , pp. 223 - 228
Copyright
© The Author(s), 2021. Published by Cambridge University Press

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References

Arai, K, Onozato, H and Yamazaki, F (1979). Artificial androgenesis induced with gamma irradiation in masu salmon, Oncorhynchus masou . Bull Fac Fish Hokkaido Univ 30, 181–6.Google Scholar
Arai, K, Ikeno, M and Suzuki, R (1995). Production of androgenetic diploid loach Misgurnus anguillicaudatus using spermatozoa of natural tetraploids. Aquaculture 137, 131–8.CrossRefGoogle Scholar
Aydın, I and Okumus, I (2017). Effects of triploid induction to the early cleavage patterns of black sea turbot (Psetta maxima) embryos. Genet Aquat Org 1, 15–20.Google Scholar
Balashov, DA, Vinogradov, EV, Kovalev, KV, Barmintseva, AE, Recoubratsky, AV and Grunina, AS (2017). Induced androgenesis in acipenserids may be obtained by ultraviolet radiation. Russ J Dev Biol 48, 330–9.CrossRefGoogle Scholar
Beardmore, JA, Mair, GC and Lewis, RI (2001). Monosex male production in finfish as exemplified by tilapia: applications, problems, and prospects. Aquaculture 197, 283–30.CrossRefGoogle Scholar
Birstein, VJ, Bemis, WE and Waldman, JR (1997). The threatened status of acipenseriform fishes: a summary. In Sturgeon Biodiversity and Conservation (ed. Birstein, VJ). Dordrecht: Kluwer Acad. Publications, pp. 427–44.CrossRefGoogle Scholar
Bongers, ABJ, in ’t Veld, EPC, Abo-Hashema, K, Bremmer, IM, Eding, EH, Komen, J and Richter, CJJ (1994). Androgenesis in common carp Cyprinus carpio (L.) using UV irradiation in a synthetic ovarian fluid and heat shocks. Aquaculture 122, 119–32.CrossRefGoogle Scholar
Bongers, ABJ, Nguenga, D, Eding, EH and Richter, CJJ (1995). Androgenesis in the African catfish, Clarias gariepinus . Aquat Living Resour 8, 329–32.CrossRefGoogle Scholar
Bongers, ABJ, Zandieh-Doulabi, B, Richter, CJ and Komen, J (1999). Viable androgenetic YY genotypes of common carp (Cyprinus carpio L.). J Hered 90, 195–8.CrossRefGoogle Scholar
Christopher, JG, Murugesan, AG and Sukumaran, N (2012). Optimization of UV treatment to induce haploid androgenesis in the stinging catfish, Heteropneustes fossilis . Int Aquat Res 4, 4.CrossRefGoogle Scholar
Christopher, JG, Murugesan, AG and Sukumaran, N (2014). Induction of diploid androgenesis in the stinging catfish, Heteropneustes fossilis. J World Aquacult Soc 45, 558–66.CrossRefGoogle Scholar
Colléter, J, Penman, DJ, Lallement, S, Fauvel, C, Hanebrekke, T, Osvik, RD, Eilertsen, HC, D’Cotta, H, Chatain, B and Peruzzi, S (2014). Genetic inactivation of European sea bass (Dicentrarchus labrax L.) eggs using UV-irradiation: observations and perspectives. PLoS One 9, p.e109572.CrossRefGoogle Scholar
Corley-Smith, GE, Lim, CJ and Brandhorst, BP (1996). Production of androgenetic zebrafish (Danio rerio). Genetics 142, 1265–76.CrossRefGoogle Scholar
Enuekwe, RC and Okonji, VA (2019). Effects of dietary 17-methyl testosterone on sex reversal and growth performance of African mud catfish (Clarias gariepinus). Niger J Fish 16, 1806–12.Google Scholar
Felip, A, Zanuy, S, Carillo, M, Martinez, G, Ramos, J and Piferrer, F (1997). Optimal condition for induction of triploidy in the sea bass. Aquaculture 152, 287–98.CrossRefGoogle Scholar
Hassan, A, Okomoda, VT and Nurhayati, MN (2018a). Embryonic development of Diploid and Triploid eggs of Clarias gariepinus (Burchell, 1822). Caryologia 71, 372–9.CrossRefGoogle Scholar
Hassan, A, Okomoda, VT and Sanusi, FA (2018b). Fertilization, hatching and embryogenesis of diploid and triploid eggs of Anabas testudineus (Bloch, 1792). Zygote 26, 343–9.CrossRefGoogle Scholar
Hertwig, O (1911). Die radium Krankheit tierischer keim zellen. Arch Mikrosk Anat 77, 1–97.CrossRefGoogle Scholar
Hou, J, Saito, T, Fujimoto, T, Yamaha, E and Arai, K (2014). Androgenetic doubled haploids induced without irradiation of eggs in loach (Misgurnus anguillicaudatus). Aquaculture 420–421, S57–63.CrossRefGoogle Scholar
Hou, JL, Wang, GX, Zhang, XY, Sun, ZH, Liu, HJ and Wang, YF (2016). Cold shock induced androgenesis without egg irradiation and subsequent production of doubled haploids and a clonal line in Japanese flounder, Paralichthys olivaceus . Aquaculture 464, 642–6.CrossRefGoogle Scholar
Hunter, GA and Donaldson, EM (1983). Hormonal sex control and its application. In Fish Physiology vol. XB (eds Hoar, WS, Randall, DJ and Donaldson, EM). Academic Press. New York, pp. 223–303.Google Scholar
Hussain, MG (1998). Manipulation of chromosomes in fish: review of various techniques and their implications in aquaculture. Bangladesh J Fish Res 2, 99–108.Google Scholar
Iegorova, V, Psenicka, M, Lebeda, I, Rodina, M and Saito, T (2018). Polyspermy produces viable haploid/diploid mosaics in sturgeon. Biol Reprod 99, 695–706.Google ScholarPubMed
Kirankumar, S and Pandian, TJ (2003). Production of androgenetic tiger barb, Puntius tetrazona . Aquaculture 228, 37–51.CrossRefGoogle Scholar
Komen, H and Thorgaard, GH (2007). Androgenesis, gynogenesis and the production of clones in fishes: a review. Aquaculture 269, 150–73.CrossRefGoogle Scholar
Kucharczyk, D, Targońska, K, Szczerbowski, A, Łuczyński, MJ, Rożek, W, Kujawa, R and Mamcarz, A (2008). Genetic inactivation of dace, Leuciscus (L.), gametes using UV irradiation. Fish Aquat Life 16, 437–46.Google Scholar
Lin, F and Dabrowski, K (1998). Androgenesis and homozygous gynogenesis in muskellunge (Esox masquinongy): evaluation using flow cytometry. Mol Reprod Dev 49, 10–8.3.0.CO;2-R>CrossRefGoogle ScholarPubMed
May, B, Henley, KJ, Krueger, CC and Gloss, SP (1988). Androgenesis as a mechanism for chromosome set manipulation in brook trout, Salvelinus fontinalis . Aquaculture 75, 57–70.CrossRefGoogle Scholar
Michalik, O, Dobosz, S, Wójcik, I, Zalewski, T and Ocalewicz, K (2014). Use of eggs derived from the interspecific charr hybrids to induce androgenetic development of the brook charr (Salvelinus fontinalis Mitchill 1814). Reprod Domest Anim 49, 191–6.CrossRefGoogle Scholar
Michalik, O, Dobosz, S, Zalewski, T, Sapota, M and Ocalewicz, K (2015). Induction of gynogenetic and androgenetic haploid and doubled haploid development in the brown trout (Salmo trutta Linnaeus 1758). Reprod Domest Anim 50, 256–62.CrossRefGoogle Scholar
Morishima, K, Fujimoto, T, Sato, M, Kawae, A, Zhao, Y, Yamaha, E and Arai, K (2011). Cold-shock eliminates female nucleus in fertilized eggs to induce androgenesis in the loach (Misgurnu anguillicaudatus), a teleost fish. BMC Biotechnol 11, 116.CrossRefGoogle Scholar
Nowosad, J, Kucharczyk, D, Liszewski, T, Targońska, K and Kujawa, R (2015). Comparison of temperature shock timing to induced artificial mitotic gynogenesis and androgenesis in common tench. Aquacult Int 23, 45–53.CrossRefGoogle Scholar
Ocalewicz, K, Dobosz, S, Kuzminski, H, Nowosad, J and Goryczko, K (2010). Chromosome rearrangements and survival of androgenetic rainbow trout (Oncorhynchus mykiss). J Appl Genet 51, 309–17.CrossRefGoogle Scholar
Ocalewicz, K, Kuzminski, H, Pomianowski, K and Dobosz, S (2013). Induction of androgenetic development of the brook charr (Salvelinus fontionalis) × Arctic charr (Salvelinus alpinus) hybrids in eggs derived from the parental species. Reprod Biol 13, 105–12.CrossRefGoogle ScholarPubMed
Ocalewicz, K, Gurgul, A, Pawlina-Tyszko, K, Szmatoła, T, Jasielczuk, I, Bugno-Poniewierska, M and Dobosz, S (2019). Induced androgenetic development in rainbow trout and transcriptome analysis of irradiated eggs. Sci Rep 9, 1–12.CrossRefGoogle ScholarPubMed
Okomoda, VT, Koh, ICC and Shahreza, MS (2018a). A simple technique for accurate estimation of fertilization rate with specific application to Clarias gariepinus (Burchell, 1822). Aquacult Res 49, 1116–21.CrossRefGoogle Scholar
Okomoda, VT, Koh, ICC, Hassan, A, Amornsakun, T and Shahreza, MS (2018b). Performance and characteristics of the progenies from the crosses of Pangasianodon hypophthalmus (Sauvage, 1878) and Clarias gariepinus (Burchell, 1822). Aquaculture 489, 96–104.CrossRefGoogle Scholar
Okomoda, VT, Koh, ICC, Hassan, A, Amornsakun, T, Moh, JH and Shahreza, MS (2018c). Optimization of the cytogenetic protocol for Pangasianodon hypophthalmus (Sauvage, 1878) and Clarias gariepinus (Burchell, 1822). Peer J 6, e5712.CrossRefGoogle Scholar
Okomoda, VT, Pradeep, PJ Oladimeji, AS, Abol-Munafi, AB, Alabi, KI, Ikhwanuddin, M, Martins, CO, Umaru, JA and Hassan, A (2020a). Effect of electric induced triploidization on sex ratio, growth and gonad histology of red hybrid tilapia. Aquaculture 520, 734991.CrossRefGoogle Scholar
Okomoda, VT, Aminath, L, Oladimeji, AS, Abol-Munafi, AB, Alabi, KI, Ikhwanuddin, M, Umaru, JA Hassan, A, Martins, CO and Shahreza, MS (2020b). First report on successful triploidy induction in Clarias gariepinus (Burchell, 1822) using electroporation. Sci Rep 10, 2425.CrossRefGoogle ScholarPubMed
Olufeagba, SO and Moses, Y (2011). Monosex production of African catfish, Heterobranchus longifilis, through gynogenesis and androgenesis. Int J Fish Aquacult 3, 65–70.Google Scholar
Onozato, H (1984). Diploidization of gynogenetically activated salmonid eggs using hydrostatic pressure. Aquaculture 43, 97.CrossRefGoogle Scholar
Pandian, TJ and Koteeswaran, R (1998). Ploidy induction and sex control in fish. Hydrobiologia 384, 167–243.CrossRefGoogle Scholar
Parsons, JE and Thorgaard, GH (1984). Induced androgenesis in rainbow trout. J Exp Zool 231, 407–12.CrossRefGoogle Scholar
Parsons, JE and Thorgaard, GH (1985). Production of androgenetic diploid rainbow trout. J Hered 76, 177–81CrossRefGoogle ScholarPubMed
Paschos, I, Natsis, L, Nathanailides, C, Kagalou, I and Kolettas, E (2001). Induction of gynogenesis and androgenesis in goldfish Carassius auratus (var. oranda). Reprod Domest Anim 36, 195–8.CrossRefGoogle Scholar
Penman, DJ (2016). Resource Use Efficiency in Fish: Application of Biotechnology in Genetic Improvement in Tropical Aquaculture. Institute of Aquaculture, University of Stirling, Scotland, UK. https://www.slideshare.net/ExternalEvents Google Scholar
Piferrer, F (2001). Endocrine sex control strategies for the feminization of teleost fish. Aquaculture 197, 229–81.CrossRefGoogle Scholar
Piferrer, F, Beaumont, A, Falguiere, J-C, Flajshans, M, Haffray, P and Colombo, L (2009). Polyploid fish and shellfish: production, biology and applications to aquaculture for performance improvement and genetic containment. Aquaculture 293, 125–56.CrossRefGoogle Scholar
Polonis, M, Jagiełło, K, Dobosz, S, Rożyński, R, Kondraciuk, P, Gurgul, A, Szmatoła, T and Ocalewicz, K (2019). Alterations in the rainbow trout (Oncorhynchus mykiss) eggs exposed to ionizing radiation during induced androgenesis. Reprod Domest Anim 54, 712–8.CrossRefGoogle ScholarPubMed
Purdom, CE (1969). Radiation-Induced gynogenesis and androgenesis in fish. Heredity 24, 431–44.CrossRefGoogle ScholarPubMed
Sule, OD, Adikwu, I (2004). Embryonic development in Clarias gariepinus (Buchell, 1822) under laboratory conditions. Anim Res Int 1, 81–5.Google Scholar
Tave, D (1992). Genetics for Fish Hatchery Managers. Van Nostrand Reinhold, New York, 336 pp.Google Scholar
Thorgaard, GH, Scheerer, PD, Hershberger, WK and Myers, JM (1990). Androgenetic rainbow trout produced using sperm from tetraploid males show improved survival. Aquaculture 85, 215–21.CrossRefGoogle Scholar
Turan, F and Akyurt, I (2005). Effects of red clover extract on growth performance and body composition of African catfish Clarias gariepinus . Fish Sci 71, 618–20.CrossRefGoogle Scholar
Volckaert, FAM, Galbusera, PHA, Hellemans, AS, Van den Haute, C, Vanstaeri, D, Ollevier, F (1994). Gynogenesis in the African catfish (Clarias gariepinus). 1. Induction of meiogynogenesis with thermal and pressure shocks. Aquaculture 123, 221–33.CrossRefGoogle Scholar
Zhou, H, Wang, Q, Liu, HJ, Chen, Q, Sun, YQ, Zheng, XY, Mahboob, S, Xu, QZ, Jiang, ZH, Zhuang, ZX and Jin, JQ (2019). Androgenetic haploid Japanese pufferfish (Takifugu rubripes) induced by cold shock. Aquacult Res 50, 3802–11.CrossRefGoogle Scholar