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Possible participation of calmodulin in the decondensation of nuclei isolated from guinea pig spermatozoa

Published online by Cambridge University Press:  26 November 2009

Armando Zepeda-Bastida ,
Natalia Chiquete-Felix ,
Juan Ocampo-López ,
Salvador Uribe-Carvajal  and
Adela Mújica
Armando Zepeda-Bastida
Affiliation:
Departamento de Biología Celular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), PC 07360, México, D. F., México.
Natalia Chiquete-Felix
Affiliation:
Departamento de Biología Celular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), PC 07360, México, D. F., México.
Juan Ocampo-López
Affiliation:
Departamento de Biología Celular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), PC 07360, México, D. F., México.
Salvador Uribe-Carvajal
Affiliation:
Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, México D. F., México.
Adela Mújica*
Affiliation:
Departamento de Biología Celular, CINVESTAV-IPN. Av. Instituto Politécnico Nacional No. 2508, México, D. F., México. Departamento de Biología Celular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), PC 07360, México, D. F., México.
*
All correspondence to: Adela Mújica. Departamento de Biología Celular, CINVESTAV-IPN. Av. Instituto Politécnico Nacional No. 2508, México, D. F., México. Tel: +5255 5747 3992. Fax: +5255 5747 3393. e-mail: adelam@cell.cinvestav.mx
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Summary

The guinea pig spermatozoid nucleus contains actin, myosin, spectrin and cytokeratin. Also, it has been reported that phalloidin and/or 2,3-butanedione monoxime retard the sperm nuclear decondensation caused by heparin, suggesting a role for F-actin and myosin in nuclear stability. The presence of an F-actin/myosin dynamic system in these nuclei led us to search for proteins usually related to this system. In guinea pig sperm nuclei we detected calmodulin, F-actin, the myosin light chain and an actin-myosin complex. To define whether calmodulin participates in nuclear-dynamics, the effect of the calmodulin antagonists W5, W7 and calmidazolium was tested on the decondensation of nuclei promoted by either heparin or by a Xenopus laevis egg extract. All antagonists inhibited both the heparin- and the X. laevis egg extract-mediated nuclear decondensation. Heparin-mediated decondensation was faster and led to loss of nuclei. The X. laevis egg extract-promoted decondensation was slower and did not result in loss of the decondensed nuclei. It is suggested that in guinea pig sperm calmodulin participates in the nuclear decondensation process.

Keywords

CalmidazoliumHeparinMyosin–actin complexW7Xenopus laevis
Type
Research Article
Information
Zygote , Volume 18 , Issue 3 , August 2010 , pp. 217 - 229
Copyright
Copyright © Cambridge University Press 2009

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