Hostname: page-component-78c5997874-dh8gc Total loading time: 0 Render date: 2024-11-10T14:24:10.888Z Has data issue: false hasContentIssue false
  • English
  • Français

Pronuclear formation and cleavage of mammalian eggs after microsurgical injection of freeze-dried sperm nuclei

Published online by Cambridge University Press:  26 September 2008

Kazuhiko Hoshi ,
Kaoru Yanagida ,
Haruo Katayose  and
Hiroyuki Yazawa
Kazuhiko Hoshi*
Affiliation:
Fukushima Medical College, Fukushima, Japan.
Kaoru Yanagida
Affiliation:
Fukushima Medical College, Fukushima, Japan.
Haruo Katayose
Affiliation:
Fukushima Medical College, Fukushima, Japan.
Hiroyuki Yazawa
Affiliation:
Fukushima Medical College, Fukushima, Japan.
*
K. Hoshi, Department of Obstetrics and Gynecology, Fukushima Medical College, Fukushima 960-12Japan. Tel: 0245-48-2111. Fax: 0245-48-3878.
Get access Rights & Permissions [Opens in a new window]

Summary

A study was conducted to evaluate the ability of mammalian oocytes to develop to the pronuclear stage and beyond if injected with freeze-dried sperm nuclei. The rate of development of hamster eggs to the pronuclear stage after microinjection of freeze-dried hamster sperm nuclei was 90%. The pronuclear formation rate of hamster eggs injected with freeze-dried human sperm nuclei was 85%. On the other hand, the rates for eggs injected with untreated sperm nuclei were 84% and 89% respectively. The survival rate of rabbit eggs microinjected with freeze-dried rabbit sperm nuclei was 64%, the fertilisation rate 56% and the cleavage rate 38%. The survival, fertilisation and cleavage rates of eggs injected with non-freeze-dried sperm nuclei were 78%, 45% and 34%, respectively. There was no difference between eggs injected with freeze-dried sperm nuclei and those injected with sperm nuclei that had not been freeze-dried. Of the viable rabbit eggs injected with freeze-dried rabbit sperm nuclei, 6% developed to the 6- to 8-cell stage 62 h after injection.

Keywords

CleavageFreeze-dryingMicrosurgical injectionpronulear formationSperm nucleus
Type
Article
Information
Zygote , Volume 2 , Issue 3 , August 1994 , pp. 237 - 242
Copyright
Copyright © Cambridge University Press 1994

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bedford, J.M. & Calvin, H.I. (1974) The occurrence and possible functional significance of –S–S– crosslinks in sperm head, with particular reference to eutherian mammals. J. Exp. Zool. 188, 137–56.CrossRefGoogle Scholar
Fishel, S., Antinori, S., Jackson, P., Lisi, F., Chiariello, F., et al. (1990). Twin birth after sub-zonal insemination. Lancet 335, 722–3.CrossRefGoogle Scholar
Goto, K. (1993). Bovine microfertilization and embryo transfer. Mol. Reprod. Dev. 36, 288–90.CrossRefGoogle ScholarPubMed
Goto, K.Kinoshita, A. & Takuma, Y. (1990). Fertilization of bovine oocytes by the injection of immobilized, killed spermatozoa. Vet. Rec. 127, 517–20.Google Scholar
Hoppe, P.C. (1985). Techniques of fertilization in vitro. In Reproductive Toxicology, ed. Dixon, R.L., pp. 191. New YorkRaven Press.Google Scholar
Hoshi, K.Yazawa, H.Yanagida, K. & Sato, A. (1992) Microinsemination of rabbit oocytes with heat-treated sperm:embryonic development Arch. Androl. 29 233–7.CrossRefGoogle ScholarPubMed
Hosoi, Y.Miyake, M.Utsumi, K. & iritani, A. (1998). Development of rabbit oocytes after microinjection of spermatozoa. Proc. 11th Int. Cong. Anim. Reprod. A.(Dublin). 3, abstr 331.Google Scholar
Iritani, A. & Hosoi, Y. (1989). Microfertilization by various methods in mammalian species. In: Development of Preimplanation Embryos and their Environment, ed. K., Yoshinaga & Mori, T., 145–9. New York: Alan R. Liss.Google Scholar
Katayose, H.Matsuda, J. & Yanagimachi, R. (1992). The ability of dehydrated hamster and human sperm nuclei to develop into pronuclei Biol. Reprod 47, 277–84.CrossRefGoogle ScholarPubMed
Keefer, C.L. (1989). Fertilization by sperm injection in the rabbit. Gamete Res 22, 59–69.Google Scholar
Lanzendorf, S.E., Maloney, M.K., Veeck, L.L., Slusser, J., Hodgen, G.D. & Rosenwaks, Z. (1988). A preclinical evaluation of pronuclear formation by microinjection of human spermatozoa into human oocytes. Fertil. Steril. 49, 835–42.CrossRefGoogle ScholarPubMed
Market, C.L. (1983). Fertilization of mammalian eggs by sperm injection. J. Exp. Zool 228, 195–201.CrossRefGoogle Scholar
Ng., S.-C.Bongso, A., Ratnam, S.S.Sathananthan, H., Chan, C.L.K.Wong, P.C.et al. (1998). Pregnancy after transfer of sperm under zona [letter]. Lancet 332 790.CrossRefGoogle Scholar
Overstreet, J.M.Yanagimachi, R.Katz, D.F.Hayashi, K. & Hanson, F.W. (1980). Penetration of human spermatoza into the human zona Pellucida and zona-free hamster egg: a study of fertile fonors and infertile patients. Fertil. Steril 33, 534–42.CrossRefGoogle Scholar
Palermo, G.Joris, H.Devroey, P. & Van Steirteghem, A.C. (1992 a) Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte. Lancet 340 17–18.Google Scholar
Palermo, G.Joris, H.Devroey, P. & Van Steirteghem, A.C. (1992 b) Induction of acrosome reaction in human spermatozoa used for subzonal insemination. Hum. Reprod 7 248–54.Google Scholar
Palermo, G.Joris, H.Derde, M.-P.Cumus, M.Devroey, P. & Steirteghem, A.V. (1993). Sperm characteristics and outcome of human assisted fertilization by subzonal insemination and intracytoplasmic sperm injection Fertil. Steril. 59, 826–35.CrossRefGoogle ScholarPubMed
Perreault, S.D. & Zirkin, B.R. (1982) Sperm nucleat decondensation in mammals:role of sperm-associated proteinase in vivo. J. Exp. Zool. 224 253–7.CrossRefGoogle ScholarPubMed
Uehara, T. & Yanagimachi, R. (1976). Micro-surgical injection of spermatozoa into hamster eggs with subsequent transformation of sperm nuclei into male pronuclei. Biol. Reprod 15 467–70.CrossRefGoogle Scholar
Uehara, T.-Yanagimachi, R. (1977). Behavior of nuclei of testicular, caput and cauda epididymal spermtozoa injected into hamster eggs. Biol. Reprod. 16 315–21.Google Scholar
Van Steirteghem, A.C., Liu, J., Joris, H., Nagy, Z., Janssenswillen, C., Tournaye, H., Derde, M.P., VanAssche, E. & Devroey, P. (1993 a). Higher success rate by intracytoplasmic sperm injection than by subzonal insemination: report of a second series of 300 consecutive treatment cycles Hum. Reprod 8 1055–60.CrossRefGoogle ScholarPubMed
Van Steirteghem, A.C.Nagy, Z.Joris, H.Liu, J.Staessen, C.Smitz, J.Wisanto, A. & Devroey, P. (1993 b) High fertilization and implantation rates after intracytoplasmic sperm injection Hum. Reprod 8 1061–6.CrossRefGoogle ScholarPubMed
Whitten, W.K. (1971) Nutrient requirements for the culture of Preimplantation embryos in vitro. In: Advances in the Bioscienciences 6 ed. Raspa, G.129–49. New York: Pergamon Press.Google Scholar
Witkowska, A. (1981) Pronuclear development and first cleavage division in Polyspermic mouse eggs. J. Reprod. Fertil. 62 493–8.Google Scholar
Yanagida, K.Yanagimachi, R.Perreault, S.D. & Kleinfeld, R.G. (1991a) Thermostability of sperm nuclei assessed by microinjection into hamster oocytes. Biol. Reprod. 44 440–7.CrossRefGoogle ScholarPubMed
Yanagida, K., Bedford, J.M. & Yanagimachi, R. (1991b). Cleavage of rabbit eggs after microsurgical injection of testicular spermatozoa. Hum. Reproad. 6 277–9.Google Scholar