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The protective effect of melatonin on the in vitro development of yak embryos against hydrogen peroxide-induced oxidative injury

Published online by Cambridge University Press:  23 April 2019

Wei Peng ,
Mengtong Lei ,
Jun Zhang  and
Yong Zhang Open the ORCID record for Yong Zhang [Opens in a new window]
Wei Peng*
Affiliation:
College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi, China
Mengtong Lei
Affiliation:
College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi, China
Jun Zhang*
Affiliation:
Institute of Veterinary Medicine, Qinghai Academy of Animal Science and Veterinary Medicine, Xining, Qinghai, China
Yong Zhang
Affiliation:
College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi, China
*
*Author for correspondence: Jun Zhang. Institute of Veterinary Medicine, Qinghai Academy of Animal Science and Veterinary Medicine, Xining, Qinghai, China. Tel:/Fax: +86 29 87080092. E-mail: zhangjunxn2003@163.com; Yong Zhang. College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi, China. E-mail: zhangy712100@yeah.net
*Author for correspondence: Jun Zhang. Institute of Veterinary Medicine, Qinghai Academy of Animal Science and Veterinary Medicine, Xining, Qinghai, China. Tel:/Fax: +86 29 87080092. E-mail: zhangjunxn2003@163.com; Yong Zhang. College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi, China. E-mail: zhangy712100@yeah.net
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Summary

Melatonin plays a critical role in several types of cells as an antioxidant to protect intracellular molecules from oxidative stress. The anti-oxidation effect of melatonin in yak embryos is largely unknown. We report that melatonin can protect the development of yak preimplantation embryos against oxidative stress induced by hydrogen peroxide (H2O2). Therefore, the quality of blastocysts developed from zygotes exposed to H2O2 was promoted. In addition, we observed that melatonin reduced H2O2-induced intracellular reactive oxygen species (ROS) levels and prevented mitochondrial dysfunction in zygotes. These phenomena revealed the effective antioxidant activity of melatonin to prevent oxidative stress in yak embryos. To determine the underlying mechanism, we further demonstrated that melatonin protected preimplantation embryos from oxidative damage by preserving antioxidative enzymes. Collectively, these results confirmed the anti-oxidation effect of melatonin in yak embryos that significantly improved the quantity and quality of blastocysts in the in vitro production of embryos in yaks.

Keywords

EmbryoOxidative stressReproductionYakZygote
Type
Research Article
Information
Zygote , Volume 27 , Issue 3 , June 2019 , pp. 118 - 125
Copyright
© Cambridge University Press 2019 

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Footnotes

†

These authors contributed equally to this study.

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