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Protein kinase C-dependent and independent events in mouse egg activation

Published online by Cambridge University Press:  26 September 2008

Rosella Colonna  and
Carla Tatone
Rosella Colonna*
Affiliation:
Dipartimento di Scienze e Tecnologie Biomediche e di Biometria, Universita′ dell'Aquila, Collemaggio, L'Aquila, Italy
Carla Tatone
Affiliation:
Dipartimento di Scienze e Tecnologie Biomediche e di Biometria, Universita′ dell'Aquila, Collemaggio, L'Aquila, Italy
*
Rosella Colonna, Dipartimento di Scienze e Tecnologie Biomediche e di Biometria, Universita′ degli Studi dell'Aquila, Collemaggio I–67100 L'Aquila, Italy. Fax: 0862–433433.
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Extract

The involvement of calcium- or protein kinase C (PKC)-dependent pathways in cortical granule exocytosis (CGE) and pronucleus formation was examined in mouse eggs using the specific PKC stimulator OAG (1-oleyl-2-acetyl-sn-glycerol) at different external calcium concentrations ([Ca2+]e) ranging from 1.7mM to 0.1μM. A 10 min exposure of eggs to 150 μM OAG in the presence of 1.7mM [Ca2+e caused a large calcium influx, cortical granule release and 82% activation. The increased permeability of the egg membrane to Ca2+ ions after OAG treatment lasted 20 min. At [Ca2+]e lower than 1.7 mM, both OAG-induced calcium influx and CGE decreased, reaching a non-detectable level at 0.1 μM and 100 μM [Ca2+]e, respectively. Resumption of meiosis was not affected by [Ca2+]e above 200 μM but it was reduced at any lower [Ca2+]e, with a minimum activation frequency of 46% at 0.1 μM [Ca2+]e. Loading of eggs with ≥3μM of the calcium chelator BAPTA AM (1,2-bis(o-aminophenox- y)ethane-N′,N′,N′,N′tetraacetic acid-acetoxymethyl ester) prior to OAG treatment caused a reduction in meiosis resumption with 50% of eggs forming pronuclei. Potent inhibitors of PKC, such as acridine orange and sphingosine, did not interfere with OAG-induced CGE. Conversely, these compounds prevented OAG-induced pronucleus formation in a dose-dependent manner with an IC50 (inhibiting concentration, 50%) of 5μM and 30 μM for acridine orange and sphingosine, respectively. Microinjection of inositol 1,4,5-trisphosphate into eggs at 0.1 pM elicited Ca2+ release from intracellular stores and the cortical reaction, but failed to stimulate pronucleus formation. These results indicate that, in mouse eggs, CGE is a PKC-independent event, and that the transition from M-phase to interphase may require PKC activity for stimulation.

Keywords

CalciumCorticalGranule exocytosisMouse eggPronucleus formationProtein kinase C
Type
Review Article
Information
Zygote , Volume 1 , Issue 3 , August 1993 , pp. 243 - 256
Copyright
Copyright © Cambridge University Press 1993

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