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Recloned transgenic pigs possess normal reproductive performance and stable genetic transmission capacity

Published online by Cambridge University Press:  12 July 2012

Zubing Cao ,
Yan Li ,
Xiao Wen ,
Zhiyuan Li ,
Changsheng Mi ,
Zaihu Zhang ,
Ning Li  and
Qiuyan Li
Zubing Cao*
Affiliation:
State Key Laboratory for Agrobiotechnology, College of Biological Science, China Agricultural University, Beijing 100193, China.
Yan Li
Affiliation:
Beijing Gene & Protein Biotechnology Co., Ltd., Beijing 100193, China.
Xiao Wen
Affiliation:
Beijing Gene & Protein Biotechnology Co., Ltd., Beijing 100193, China.
Zhiyuan Li
Affiliation:
Beijing Gene & Protein Biotechnology Co., Ltd., Beijing 100193, China.
Changsheng Mi
Affiliation:
Beijing Gene & Protein Biotechnology Co., Ltd., Beijing 100193, China.
Zaihu Zhang
Affiliation:
Beijing Gene & Protein Biotechnology Co., Ltd., Beijing 100193, China.
Ning Li
Affiliation:
State Key Laboratory for Agrobiotechnology, College of Biological Science, China Agricultural University, Beijing 100193, China.
Qiuyan Li*
Affiliation:
State Key Laboratory for Agrobiotechnology, College of Biological Science, China Agricultural University, Beijing 100193, China.
*
All correspondence to: Zubing Cao. State Key Laboratory for Agrobiotechnology, College of Biological Science, China Agricultural University, Beijing 100193, China. Tel:/Fax: +86 10 62731142. e-mail: caozubing@163.com
Qiuyan Li, State Key Laboratory for Agrobiotechnology, College of Biological Science, China Agricultural University, Beijing 100193, China. Tel:/Fax: +86 10 62731143. e-mail: liqiuyan@cau.edu.cn
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Summary

The present study investigated whether a recloning procedure would affect the reproductive performance or the germline transmission capacity of recloned transgenic pigs. This study has also laid the foundation for the development of elite transgenic swine breeds in the future. Recloned transgenic pigs were developed from ear tissue fibroblasts of primary transgenic cloned pigs using a recloning procedure, and their reproductive performance and exogenous gene transmission were analyzed. Two transgenic cell lines with different genetic backgrounds (derived from a female miniature pig and a male Landrace pig) with stable expression of green fluorescent protein (GFP) were established successfully. Furthermore, recloned transgenic embryos were developed to full term successfully. One female Chinese experimental miniature piglet (CEMP) (GFP+) and three male Landrace piglets (GFP+) were delivered naturally. Furthermore, the index values for the reproductive characteristics of the recloned transgenic pigs, such as puberty, gestation period, sperm volume and sperm concentration, were not significantly different from those of conventionally bred pigs. In addition, 53% of the F1 offspring of the recloned transgenic pigs were GFP positive. These results demonstrate that ear tissue fibroblasts from primary transgenic cloned pigs efficiently support the full-term development of recloned transgenic embryos. Furthermore, recloned transgenic pigs maintain normal reproductive performance and stable germline (genetic) transmission capacities.

Keywords

Genetic transmissionPigRecloningReproductive performanceTransgene
Type
Research Article
Information
Zygote , Volume 22 , Issue 1 , February 2014 , pp. 18 - 24
Copyright
Copyright © Cambridge University Press 2012 

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